Effect of Lactic Acid Bacteria and Molasses on the Silage Quality and Bacterial Diversity of Paper Mulberry

doi:10.11733/j.issn.1007-0435.2023.01.032

Acta Agrestia Sinica ›› 2023, Vol. 31 ›› Issue (1): 280-286.DOI: 10.11733/j.issn.1007-0435.2023.01.032

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Effect of Lactic Acid Bacteria and Molasses on the Silage Quality and Bacterial Diversity of Paper Mulberry

SUN Rong¹, LIU Yue¹, CHEN Ting¹, LYU Ren-long², ZHOU Han-lin², LI Mao², ZI Xue-juan¹

1. College of forestry, Hainan University, Danzhou, Hainan Province 571737, China;
2. Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, Hainan Province 571737, China;
3. Zhanjiang Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, Guangdong Province 524000, China

Received:2022-07-20 Revised:2022-09-07 Online:2023-01-15 Published:2023-02-07

乳酸菌和糖蜜对构树叶青贮品质和细菌多样性的影响

孙蓉¹, 刘悦¹, 陈婷¹, 吕仁龙², 周汉林², 李茂², 字学娟¹

1. 海南大学林学院, 海南儋州 571737;
2. 中国热带农业科学院热带作物品种资源研究所, 海南儋州 571737;
3. 中国热带农业科学院湛江试验站, 广东湛江, 524000

通讯作者: 李茂, E-mail:limaohn@163.com;字学娟, E-mail:zixuejuan@163.com
作者简介:孙蓉(2000-)，女，汉族，海南儋州人，硕士研究生，主要从事木本饲料加工调制研究，E-mail:sunrong9377@163.com
基金资助:
国家自然科学基金项目(31860680)资助

Abstract

Abstract: To improve the fermentation quality of paper mulberry silage,the effects of lactic acid bacteria and molasses on the fermentation index and bacterial diversity of mulberry leaf silage were investigated. In this experiment,Broussonetia papyrifera leaves were used as ensiling materials,four treatments were carried out as follows:control (CK),lactic acid bacteria (L),molasses (M),and lactic acid bacteria in combination with molasses (ML). After 30 days,the nutritional composition,silage quality,and bacterial community structure were determined. The results showed that except for the added lactic acid bacteria group,the pH of other additive treatments was significantly decreased (P<0.05),increased lactic acid content (P<0.05),and decreased acetic acid and propionic acid content (P<0.05). Microbial analysis showed that compared with the CK group,the bacterial diversity was raised in the M and ML group. In addition,the silage bacterial community structure was changed by additive treatment. Compared with CK,the abundance of Lactobacillus in the molasses treatment group (M) was increased significantly,meanwhile,Weissella and Enterobacter were decreased. In conclusion,adding molasses can improve the fermentation quality of paper mulberry silage and change the bacterial diversity of the silage.

Key words: Broussonetia papyrifera leaves, Lactic acid bacteria, Molasses, Silage quality, Bacterial diversity

摘要： 为改善构树(Broussonetia papyrifera)叶青贮发酵品质，探究乳酸菌和糖蜜对构树叶青贮发酵指标和细菌多样性的影响，本实验以构树叶为原料，设置对照、添加乳酸菌(L)、糖蜜(M)和乳酸菌+糖蜜混合(ML)4个处理，贮藏30天后，测定其营养成分、发酵品质并及细菌群落结构。结果显示，除了添加乳酸菌组，其他添加剂处理pH值均显著降低(P<0.05)，乳酸含量提高(P<0.05)，乙酸(P<0.05)和丙酸含量下降。微生物分析表明与CK组相比，M和ML组均提高了细菌群落多样性，另外，添加剂处理改变了细菌群落结构，与CK相比，糖蜜处理组(M)乳杆菌属(Lactobacillus)相对丰度显著提升，魏斯氏菌属(Weissella)和肠杆菌属(Enterobacter)相对丰度降低。综上，构树叶青贮添加糖蜜可提高青贮饲料的发酵品质，并改变青贮细菌多样性。

关键词: 构树叶, 乳酸菌, 糖蜜, 青贮发酵品质, 细菌多样性

CLC Number:

SUN Rong, LIU Yue, CHEN Ting, LYU Ren-long, ZHOU Han-lin, LI Mao, ZI Xue-juan. Effect of Lactic Acid Bacteria and Molasses on the Silage Quality and Bacterial Diversity of Paper Mulberry[J]. Acta Agrestia Sinica, 2023, 31(1): 280-286.

孙蓉, 刘悦, 陈婷, 吕仁龙, 周汉林, 李茂, 字学娟. 乳酸菌和糖蜜对构树叶青贮品质和细菌多样性的影响[J]. 草地学报, 2023, 31(1): 280-286.

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References

[1] 彭海龙,江书忠,罗平成. 构树在养猪生产上的应用研究[J]. 饲料博览,2018(12):52-58 PENG H L,JIANG S Z,LUO P C. Application of Broussonetia Papyrifera in Pig Production[J]. Feed Review,2018(12):52-58
[2] 许健. 新型饲料——构树[J]. 中国牧业通讯,2004(22):63-65 XU J.A New Type of Feed—Paper Mulberry[J]. China Animal Husbandry Bulletin,2004(22):63-65
[3] 司丙文,徐文财,张小利,等. 不同添加剂对杂交构树青贮发酵品质的影响[J]. 动物营养学报,2018,30(11):4670-4675 SI B W,XU W C,ZHANG X L,et al. Influence of Different Additives on Fermentation Quality of Hybrid Paper Mulberry Silage[J]. Chinese Journal of Animal Nutrition,2018,30(11):4670-4675
[4] 刘凯丽. 不同含水量及添加剂对杂交构树青贮饲料品质的影响[D]. 太谷:山西农业大学,2019:23-25 LIU K L. Effects of Different Water Content and Additives on Quality of Hybrid Paper Mulberry Silage[D]. Taigu:Shanxi Agricultural University,2019:23-25
[5] 付锦涛,王学凯,倪奎奎,等. 添加乳酸菌和糖蜜对全株构树和稻草混合青贮的影响[J]. 草业学报,2020,29(4):121-128 FU J T,WANG X K,NI K K,et al. The Effects of Adding Lactic Acid Bacteria and Molasses on Fermentation of Broussonetia Papyrifera and Rice Straw Mixed Silage[J]. Acta Prataculturae Sinica,2020,29(4):121-128
[6] 李文杨,陈鑫珠,张晓佩,等. 不同添加剂对杂交狼尾草青贮品质的影响[J]. 中国草食动物科学,2016(1):37-40 LI W Y,CHEN X Z,ZHANG X P,et al. Effect of Additives on the Quality of Hybrid Pennisetum Silage[J]. China Herbivore Science,2016(1):37-40
[7] MORDENTI A L,GIARETTA E,CAMPIDONICO L,et al. A Review Regarding the Use of Molasses in Animal Nutrition[J]. Animals,2021,11(1):115
[8] ZI X J,LI M,CHEN Y Y,et al. Effects of Citric Acid and Lactobacillus Plantarum on Silage Quality and Bacterial Diversity of King Grass Silage[J]. Frontiers in Microbiology,2021,12:362-362
[9] LI M,ZI X J,ZHOU H L,et al. Effects of Sucrose,Glucose,Molasses and Cellulase on Fermentation Quality and in Vitro Gas Production of King Grass Silage[J]. Animal Feed Science and Technology,2014,06(16):206-212
[10] 商振达,谭占坤,李家奎,等. 种植时间对西藏地区青贮玉米发酵品质和微生物多样性的影响[J]. 草地学报,2019,27(2):487-496 SHANG Z D,TAN Z K,LI J K,et al. Effects of Different Planting Seasons on the Fermentation Quality and Microbial Diversity of Corn Silage in Tibet[J]. Acta Agrestia Sinica,2019,27(2):487-496
[11] 董晓玲,孟庆翔. 高油玉米秸秆青贮发酵动态及青贮浸提液对活体外瘤胃发酵的影响[J]. 中国农业大学学报,2006,11(5):35-40 DONG X L,MENG Q X.Silage Fermentation Dynamics and in Vitro Rumen Fermentation Characteristics as Affected by Supplementation of Slurry Fluid Extracted from High Oil Corn Stalk Silage[J]. Journal of China Agricultural University,2006,11(5):35-40
[12] 李茂,字学娟,周汉林,等. 不同添加剂对柱花青贮品质大的影响[J]. 热带作物学报,2012,33(4):726-729 LI M,ZI X J,ZHOU H L,et al. The Effect of Different Silage Additives on the Quality of Stylo Silage[J]. Chinese Journal of Tropical Crops,2012,33(4):726-729
[13] 王超,张永虎,杨飞雁,等. 添加糖蜜和植物乳杆菌对谷草黄贮发酵品质的影[J].畜牧与饲料科学,2021,42(3):28-31 WANG C,ZHANG Y H,YANG F Y,et al. Effects of Adding Molasses and Lactobacillus Plantarum on Fermentation Quality of Yellow Millet Straw Silage[J]. Animal Husbandry and Feed Science,2021,42(3):28-31
[14] 于杰,郑琛,李发弟,等. 向日葵秸秆与全株玉米混合青贮饲料品质评定[J].草业学报,2013,22(5):198-204 YU J,ZHENG C,LI F D,et al. An Evaluation of Mixed-Ensiling Sunflower Straw and Whole Corn on Silage Quality[J]. Acta Prataculturae Sinica,2013,22(5):198-204
[15] 华金玲,张永根,王德福,等. 添加乳酸菌制剂对水稻秸青贮品质的影响[J]. 东北农业大学学报,2007,38(4):61-64 HUA J L,ZHANG Y G,WANG D F,et al. Effects of Lactic Acid Bacteria on Quality of Rice Straw Silage[J]. Journal of Northeast Agricultural University,2007,38(4):61-64
[16] 穆麟,李顺,曾宁波,等. 添加糖蜜、乳酸菌制剂对籽粒苋与稻秸混合青贮品质的影响[J]. 草地学报,2019,27(2):482-487 MU L,LI S,ZENG N B,et al. Effect of Adding Molasses or Lactic Acid Bacteria on Quality of Mixed Silage of Amaranth and Rice Straws[J]. Acta Agrestia Sinica,2019,27(2):482-487
[17] 朱见深,胡明,齐静,等. 基于高通量测序分析青贮玉米微生物多样性[J]. 山东农业科学,2020,52(4):68-72 ZHU J S,HU M,QI J,et al. Analysis of Microbial Diversity of Maize Silage by High-Throughput Sequencing[J]. Shandong Agricultural Sciences,2020,52(4):68-72
[18] RANJT N K,TAYLOR C C,KUNG L,et al.Effect of Lactobacillus Buchneri 40788 on the Fermentation,Aerobic Stability and Nutritive Value of Maize Silage[J].Grass and Forage Science,2010,57(2):73-81
[19] 孙安琪. 白酒糟与菊芋渣混合青贮发酵品质及微生物多样性研究[D]. 兰州:兰州理工大学,2019:24-52 SUN A Q. Study on Fermentation Quality and Microbial Diversity of Jerusalem Artichoke Residue and Grain Stillage Mixed Silage[D]. Lanzhou:Lanzhou University of Technolog,2019:24-52
[20] 王应芬,李龙兴,张明均,等. 酶和乳酸菌对多年生黑麦草与玉米秸秆混合青贮发酵品质的影响[J]. 中国农学通报,2017,33(5):107-111 WANG Y F,LI L X,ZHANG M J,et al. Enzyme and Lactic Acid Bacteria Affecting Fermentation Quality of Mixed Silage of Perennial Ryegrass and Corn Straw[J]. Chinese Agricultural Science Bulletin,2017,33(5):107-111
[21] 陶莲,刁其玉. 青贮发酵对玉米秸秆品质及菌群构成的影响[J]. 动物营养学报,2016,28(1):198-207 TAO L,DIAO Q Y. Effects of Ensiling on Quality and Bacteria Composition of Corn Stalk[J]. Chinese Journal of Animal Nutrition,2016,28(1):198-207
[22] EIKMEYER F G,KÖFINGER P,POSCHENEI A,et al. Metagenome Analyses Reveal the Influence of the Inoculant Lactobacillus Buchneri CD034 on the Microbial Community Involved Ingrass Ensiling[J]. Journal of Biotechnology,2013,167(3):334-343
[23] MCGARVEY J A,FRANCO R B,PALUMBO J D,et al. Bacterial Population Dynamics during the Ensiling of Medicago Sativa and Subsequent Exposure to Air[J]. Journal of Applied Microbiology,2013,114(6):1661-1670
[24] 王新宇,申晨,霍文婕,等. 雁北地区不同品种玉米全株青贮发酵品质及细菌群落结构分析[J]. 草地学报,2021,29(5):1087-1093 WANG X Y,SHEN C,HUO W J,et al. Analysis of Fermentation Quality and Bacterial Community Structure of Whole Corn Silage of Different Varieties in Yanbei Region[J]. Acta Agrestia Sinica,2021,29(5):1087-1093
[25] 龙仕和,李雪枫,潘俊歆,等. 不同生长天数'热研4号’王草青贮品质和微生物群落多样性研究[J]. 草地学报,2022,30(7):1900-1908 LONG S H,LI X F,PAN J X,et al. Study on the Silage Quality and Microbial Community Diversity of 'Reyan No.4’ King Grass Harvested at Different Growth Days[J]. Acta Agrestia Sinica,2022,30(7):1900-1908
[26] 张想峰,艾尼瓦尔·艾山,买热木尼沙·吾普尔,等. 新疆小芦苇青贮中乳酸杆菌的分离鉴定[J]. 新疆农业科学,2009,46(6):1327-1331 ZHANG X F,ANNIWAER·A S,MAIERMUNISHA·W P E,et al. Isolation and Identification of Lactobacillus from Xinjiang Small Reed Silage[J]. Xinjiang Agricultural Sciences,2009,46(6):1327-1331
[27] 杨杨,石超,郭旭生,等. 高原草甸魏斯氏乳酸菌的分离鉴定及理化特性研究[J]. 草业学报,2014,23(1):266-275 YANG Y,SHI C,GUO X S,et al. Characterization and Identification of Weissella Species Isolated from Kobresia Littledalei Growing in Alpine Meadows[J]. Acta Prataculturae Sinica,2014,23(1):266-275

Effect of Lactic Acid Bacteria and Molasses on the Silage Quality and Bacterial Diversity of Paper Mulberry

乳酸菌和糖蜜对构树叶青贮品质和细菌多样性的影响

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