Effects of Adding Lactiplantibacillus plantarum and Lactobacillus buchneri on Silage Fermentation Quality and Aerobic Stability of Pennisetum glaucum×purpureum

doi:10.11733/j.issn.1007-0435.2023.12.033

Acta Agrestia Sinica ›› 2023, Vol. 31 ›› Issue (12): 3867-3875.DOI: 10.11733/j.issn.1007-0435.2023.12.033

Previous Articles Next Articles

Effects of Adding Lactiplantibacillus plantarum and Lactobacillus buchneri on Silage Fermentation Quality and Aerobic Stability of Pennisetum glaucum×purpureum

GAO Long¹, ZHANG Juan¹, GE Chen-yan¹, LIU Kuan¹, TANG Jia-qi¹, SHI Shen-bing², YANG Fu-lin¹, ZHOU Jing³

1. College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou, Fujian Province 350002, China;
2. Nanping Jianyang District Jixiang Animal Husbandry Co.Ltd, Nanping, Fujian Province 354207, China;
3. China National Engineering Research Center of JUNCAO Technology, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province 350002, China

Received:2023-06-21 Revised:2023-08-25 Online:2023-12-15 Published:2024-01-03

添加植物乳杆菌、布氏乳杆菌对杂交狼尾草青贮发酵品质及有氧稳定性的影响

高龙¹, 张娟¹, 戈辰妍¹, 刘宽¹, 唐嘉祺¹, 石神炳², 阳伏林¹, 周晶³

1. 福建农林大学动物科学学院(蜂学学院), 福建福州 350002;
2. 南平市建阳区吉翔牧业有限公司, 福建南平 354207;
3. 福建农林大学国家菌草工程技术研究中心, 福建福州 350002

通讯作者: 阳伏林,E-mail:fulin.yang@fafu.edu.cn;周晶,E-mail:zhoujing_lz@hotmail.com
作者简介:高龙(1997-)，男，汉族，新疆伊犁人，硕士研究生，主要从事动物营养与饲料科学研究，E-mail：1194746853@qq.com；张娟(1996-)，女，汉族，陕西延安人，硕士研究生，主要从事动物营养与饲料科学研究，E-mail：2682965738@qq.com
基金资助:
福建省科技特派员后补助项目(2022S2071)；福建农林大学杰出青年科研人才计划项目(xjq201817)资助

Abstract

Abstract: The aim of study was to explore the effects of Lactiplantibacillus plantarum and Lactobacillus buchneri on the silage fermentation quality and aerobic stability of Pennisetum glaucum×purpureum. Four groups were established as follows:CK group (no addition),Lactobacillus plantarum group (LP group,1×10⁶cfu·g^-1 FW),Lactobacillus buchneri group (LB group,1×10⁶ cfu·g^-1 FW),and Lactiplantibacillus plantarum and Lactobacillus buchneri group (LPLB group,5×10⁵ cfu·g^-1 LP+ 5×10⁵ cfu·g^-1LB). After 60 days of fermentation,the fermentation quality and aerobic stability of all the Pennisetum glaucum×purpureum silage were measured. The results showed that:Compared with the CK group,the addition of Lactic acid bacteria significantly improved the crude protein (CP) content in all treatment groups (P<0.05). The pH value and butyric acid (BA) content of Lactic acid bacteria treatment were significantly lower than those of CK group (P<0.05),while the contents of lactic acid (LA) and acetic acid (AA) were significantly higher than those of CK group (P<0.05). During the aerobic exposure,the content of LA was significantly higher than that of CK group (P<0.05). The content of ammonia nitrogen was lower than that of CK group (P<0.05). After 9 days of aerobic exposure,yeast and aerobic bacteria numbers significantly reduced,while lactic acid bacteria numbers significantly increased in LB and LPLB groups (P<0.05). The comprehensive value evaluation by the membership function method found that LPLB group had the best comprehensive performance of silage effect.

Key words: Pennisetum glaucum×purpureum, Lactobacillus plantarum, Lactobacillus buchneri, Fermentation quality, Aerobic stability

摘要： 为探究植物乳杆菌、布氏乳杆菌对杂交狼尾草(Pennisetum glaucum×purpureum)青贮发酵品质及有氧稳定性的影响，本试验以杂交狼尾草为试验材料，设置对照组(CK组，无添加)、植物乳杆菌(Lactiplantibacillus plantarum)组(LP组，1×10⁶ cfu·g^-1 FW)、布氏乳杆菌(Lactobacillus buchneri)组(LB组，1×10⁶ cfu·g^-1FW)、植物乳杆菌和布氏乳杆菌组(LPLB组，5×10⁵ cfu·g^-1 FW LP+5×10⁵ cfu·g^-1 FW LB)，厌氧发酵60天(d)后，测定其营养成分、发酵品质和有氧稳定性。结果表明：与CK组相比，各处理组粗蛋白(CP)、乳酸(LA)和乙酸(AA)含量显著增加(P<0.05)，pH值和丁酸(BA)含量显著下降(P<0.05)；在有氧暴露阶段，各处理组乳酸(LA)含量显著增加(P<0.05)，氨态氮含量显著降低(P<0.05)，LB和LPLB组在有氧暴露9天时，酵母菌和好氧菌数量(P<0.05)显著降低，乳酸菌数量显著增加(P<0.05)；用隶属函数法进行综合价值评定，LPLB组青贮效果最佳。

关键词: 杂交狼尾草, 植物乳杆菌, 布氏乳杆菌, 发酵品质, 有氧稳定性

CLC Number:

S816.5+3

GAO Long, ZHANG Juan, GE Chen-yan, LIU Kuan, TANG Jia-qi, SHI Shen-bing, YANG Fu-lin, ZHOU Jing. Effects of Adding Lactiplantibacillus plantarum and Lactobacillus buchneri on Silage Fermentation Quality and Aerobic Stability of Pennisetum glaucum×purpureum[J]. Acta Agrestia Sinica, 2023, 31(12): 3867-3875.

高龙, 张娟, 戈辰妍, 刘宽, 唐嘉祺, 石神炳, 阳伏林, 周晶. 添加植物乳杆菌、布氏乳杆菌对杂交狼尾草青贮发酵品质及有氧稳定性的影响[J]. 草地学报, 2023, 31(12): 3867-3875.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2023.12.033

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2023/V31/I12/3867

References

[1] ZHAO J,XIA B,MENG Y,et al. Transcriptome Analysis to Shed Light on the Molecular Mechanisms of Early Responses to Cadmium in Roots and Leaves of King Grass (Pennisetum americanum×P. purpureum)[J]. International Journal of Molecular Sciences,2019,20(10):2532
[2] ZHENG J,WANG L,HOU W,et al. Fusarium oxysporum Associated with Fusarium Wilt on Pennisetum sinese in China[J]. Pathogens,2022,11(9):999
[3] 胡远彬,梁小玉,易军,等. 混合比例和添加剂对象草和全株玉米混合青贮品质的影响[J]. 草业科学,2022,39(4):778-786
[4] 李鑫琴,樊杨,田静,等. 中国南方青贮饲料研究进展[J]. 中国草地学报,2022,44(6):106-114
[5] ZHAO S,YANG F,WANG Y,et al. Dynamics of Fermentation Parameters and Bacterial Community in High-Moisture Alfalfa Silage with or without Lactic Acid Bacteria[J]. Microorganisms,2021,9(6):1225
[6] 冼振宇,李苏敏,池宙,等. 葡萄糖和苹果酸对杂交狼尾草青贮发酵品质和营养成分的影响[J]. 中国草地学报,2022,44(2):116-120
[7] 李珏,韩雪林,冯启贤,等. 益生菌添加剂对紫花苜蓿和杂交狼尾草以及二者混合青贮营养品质、发酵品质的影响[J]. 动物营养学报,2023,35(6):4057-4069
[8] 何玮,冉启凡. 添加纤维素酶和乳酸菌对'桂牧1号'杂交狼尾草青贮品质的影响[J]. 中国饲料,2022(3):129-132
[9] ZAYED M S,TAHA E A,HEGAZY F H,et al. Influence of Effective Microorganisms on Some Biological and Biochemical Aspects of Spodoptera littoralis (Boisduval) (Lepidoptera:Noctuidae)[J]. Life,2022,12(11):1726
[10] DONG M,LI Q,XU F,et al. Effects of microbial inoculants on the fermentation characteristics and microbial communities of sweet sorghum bagasse silage[J]. Scientific Reports,2020,10(1):837
[11] CAO C,BAO W,LI W,et al. Changes in physico-chemical characteristics and viable bacterial communities during fermentation of alfalfa silages inoculated with Lactobacillus plantarum[J]. World Journal of Microbiology and Biotechnology,2021,37(7):127
[12] HUO W,WANG X,WEI Z,et al. Effect of lactic acid bacteria on the ensiling characteristics and in vitro ruminal fermentation parameters of alfalfa silage[J]. Italian Journal of Animal Science,2021,20(1):623-631
[13] 周枫,靳羽慧,马梦婷,等. 基于植物乳杆菌处理的南湾鳙鱼肉在贮藏期内的品质变化[J]. 现代食品科技,2022,38(10):156-161
[14] YANG F,WANG Y,ZHAO S,et al. Lactobacillus plantarum Inoculants Delay Spoilage of High Moisture Alfalfa Silages by Regulating Bacterial Community Composition[J]. Frontiers in Microbiology,2020,11:1989
[15] LI Y,DU S,SUN L,et al. Effects of Lactic Acid Bacteria and Molasses Additives on Dynamic Fermentation Quality and Microbial Community of Native Grass Silage[J]. Frontiers in Microbiology,2022,13:830121
[16] XU S,YANG J,QI M,et al. Impact of Saccharomyces cerevisiae and Lactobacillus buchneri on microbial communities during ensiling and aerobic spoilage of corn silage[J]. Journal of Animal Science,2019,97(3):1273-1285
[17] XU D,DING W,KE W,et al. Modulation of Metabolome and Bacterial Community in Whole Crop Corn Silage by Inoculating Homofermentative Lactobacillus plantarum and Heterofermentative Lactobacillus buchneri[J]. Frontiers in Microbiology,2019,9:3299
[18] MUCK R E,NADEAU E M G,MCALLISTER T A,et al. Silage review:Recent advances and future uses of silage additives[J]. Journal of Dairy Science,2018,101(5):3980-4000
[19] ZHANG F,MIAO F,WANG X,et al. Effects of homo- and hetero-fermentative lactic acid bacteria on the quality and aerobic stability of corn silage[J]. Canadian Journal of Animal Science,2021,101(4):761-770
[20] 张述伟,宗营杰,方春燕,等. 蒽酮比色法快速测定大麦叶片中可溶性糖含量的优化[J]. 食品研究与开发,2020,41(7):196-200
[21] 张娟,史文娇,韩雪林,等. 苹果酸和嗜酸乳杆菌对圆叶决明青贮品质及微生物多样性的影响[J]. 动物营养学报,2021,33(12):6941-6952
[22] KUNG L,SHAVER R D,GRANT R J,et al. Silage review:Interpretation of chemical,microbial,and organoleptic components of silages[J]. Journal of Dairy Science,2018,101(5):4020-4033
[23] 柳俊超,姜俊芳,宋雪梅,等. 青贮饲料中主要有机酸的高效液相色谱分离分析[J]. 畜牧与兽医,2015,47(10):47-52
[24] 冯启贤,张磊,李妍,等. 甲酸处理对苜蓿青贮品质及瘤胃降解率的影响[J]. 草地学报,2023,31(4):1264-1272
[25] 凌文卿,张磊,李珏,等. 布氏乳杆菌和不同糖类联用对紫花苜蓿青贮营养成分、发酵品质、瘤胃降解率及有氧稳定性的影响[J]. 草业学报,2023,32(7):122-134
[26] BAI J,DING Z T,SU R N,et al. Storage Temperature Is More Effective Than Lactic Acid Bacteria Inoculations in Manipulating Fermentation and Bacterial Community Diversity,Co-Occurrence and Functionality of the Whole-Plant Corn Silage[J]. Microbiology Spectrum,2022,10(2):101-122
[27] KAEWPILA C,GUNUN P,KESORN P,et al. Improving ensiling characteristics by adding lactic acid bacteria modifies in vitro digestibility and methane production of forage-sorghum mixture silage[J]. Scientific Reports,2021,11(1):8049
[28] DA SILVA E B,SMITH M L,SAVAGE R M,et al. Effects of Lactobacillus hilgardii 4785 and Lactobacillus buchneri 40788 on the bacterial community,fermentation and aerobic stability of high-moisture corn silage[J]. Journal of Applied Microbiology,2021,130(5):1481-1493
[29] 司丙文,徐文财,张小利,等. 不同添加剂对杂交构树青贮发酵品质的影响[J]. 动物营养学报,2018,30(11):4670-4675
[30] 张嘉懿,格根图,贾玉山,等. 不同乳酸菌添加剂对苦荬菜青贮品质的影响[J]. 草地学报,2022,30(12):3433-3438
[31] CHEN L,BAO X,GUO G,et al. Effects of Hydrolysable Tannin with or without Condensed Tannin on Alfalfa Silage Fermentation Characteristics and In Vitro Ruminal Methane Production,Fermentation Patterns,and Microbiota[J]. Animals,2021,11(7):1967
[32] 司华哲,刘晗璐,南韦肖,等. 不同发酵类型乳酸菌对低水分粳稻秸秆青贮发酵品质及有氧稳定性的影响[J]. 草地学报,2017,25(6):1294-1299
[33] KUNG L,SCHMIDT R J,EBLING T E,et al. The effect of Lactobacillus buchneri 40788 on the fermentation and aerobic stability of ground and whole high-moisture corn[J]. Journal of Dairy Science,2007,90(5):2309-2314
[34] 毛翠,刘方圆,宋恩亮,等. 不同乳酸菌添加量和发酵时间对全株玉米青贮营养价值及发酵品质的影响[J]. 草业学报,2020,29(10):172-181
[35] DU S,YOU S,JIANG X,et al. Evaluating the fermentation characteristics,bacterial community,and predicted functional profiles of native grass ensiled with different additives[J]. Frontiers in Microbiology,2022,13:1025536
[36] 孙向丽,菅复春,许来鹏,等. 布氏乳杆菌和植物乳杆菌对高粱青贮品质的影响[J]. 中国畜牧杂志,2021,57(9):159-163
[37] HUANG Z,WANG M,KE W,et al. Screening of High 1,2-Propanediol Production by Lactobacillus buchneri Strains and Their Effects on Fermentation Characteristics and Aerobic Stability of Whole-Plant Corn Silage[J]. Agriculture,2021,11(7):590
[38] REN H,WANG C,FAN W,et al. Effects of Formic or Acetic Acid on the Storage Quality of Mixed Air-Dried Corn Stover and Cabbage Waste,and Microbial Community Analysis[J]. Food Technology and Biotechnology,2018,56(1):71-82
[39] JESUS D L S D,SAMPAIO J P R,MONCAO F P,et al. Nutritive value of sugarcane silages added with increasing levels of acetic acid[J]. Semina-Ciencias Agrarias,2019,40(5):2387-2396
[40] TAO X,JI C,CHEN S,et al. Fermentation quality and aerobic stability of Napier grass ensiled with citric acid residue and lactic acid bacteria[J]. Tropical Grasslands-Forrajes Tropicales,2021,9(1):52-59
[41] WALI A,HOU J,TSURUTA T,et al. Bacterial and fungal microbiota of total mixed ration silage stored at various temperatures[J]. Journal of Applied Microbiology,2022,133(2):579-590
[42] 陆永祥,赵嫚,陈良寅,等. 布氏乳杆菌和甲酸对青藏高原不同物候期燕麦青贮饲料发酵品质和细菌群落的影响[J]. 草地学报,2020,28(6):1736-1743
[43] SILVEIRA R C H,HARTER C J,DA SILVA AVILA C L,et al. Meta-analysis of the effects of Lactobacillus plantarum and Lactobacillus buchneri on fermentation,chemical composition and aerobic stability of sugarcane silage[J]. Grassland Science,2019,65(1):3-12
[44] WU B,HU Z,WEI M,et al. Effects of inoculation of Lactiplantibacillus plantarum and Lentilactobacillus buchneri on fermentation quality,aerobic stability,and microbial community dynamics of wilted Leymus chinensis silage[J]. Frontiers in Microbiology,2022,13:928731
[45] XU D,DING Z,WANG M,et al. Characterization of the microbial community,metabolome and biotransformation of phenolic compounds of sainfoin (Onobrychis viciifolia) silage ensiled with or without inoculation of Lactobacillus plantarum[J]. Bioresource Technology,2020,316:123910
[46] KUPRYS-CARUK M,CHOINSKA R,DEKOWSKA A,et al. Silage quality and biogas production from Spartina pectinata L. fermented with a novel xylan-degrading strain of Lactobacillus buchneri M B/00077[J]. Scientific Reports,2021,11(1):13175
[47] DA SILVA N C,NASCIMENTO C F,CAMPOS V M A,et al. Influence of storage length and inoculation with Lactobacillus buchneri on the fermentation,aerobic stability,and ruminal degradability of high-moisture corn and rehydrated corn grain silage[J]. Animal Feed Science and Technology,2019,251:124-133
[48] HOOKER K,FORWOOD D L,CARO E,et al. Microbial characterization and fermentative characteristics of crop maize ensiled with unsalable vegetables[J]. Scientific Reports,2019,9(1):13183
[49] WANG M,YU Z,WU Z,et al. Effect of Lactobacillus plantarum 'KR107070' and a propionic acid-based preservative on the fermentation characteristics,nutritive value and aerobic stability of alfalfa-corn mixed silage ensiled with four ratios[J]. Grassland Science,2018,64(1):51-60
[50] FERRERO F,TABACCO E,PIANO S,et al. Temperature during conservation in laboratory silos affects fermentation profile and aerobic stability of corn silage treated with Lactobacillus buchneri,Lactobacillus hilgardii,and their combination[J]. Journal of Dairy Science,2021,104(2):1696-1713
[51] OKOYE C O,WANG Y,GAO L,et al. The performance of lactic acid bacteria in silage production:A review of modern biotechnology for silage improvement[J]. Microbiological Research,2023,266:127212
[52] LI M,ZHOU S,TANG X,et al. Effect of co-inoculation ofLactobacillus plantarum and Lentilactobacillus buchneri on aerobic stability and microbial community composition of perennial sorghum silage[J]. Biomass and Bioenergy,2023,173:106801

Effects of Adding Lactiplantibacillus plantarum and Lactobacillus buchneri on Silage Fermentation Quality and Aerobic Stability of Pennisetum glaucum×purpureum

添加植物乳杆菌、布氏乳杆菌对杂交狼尾草青贮发酵品质及有氧稳定性的影响

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	YANG Dan, WU Shuo, CHEN Dan-dan, ZHANG Qing. Determination of the Active Components of Essential Oil in Neolamarckia cadamba and Its Effect on the Aerobic Stabilization of Whole Plant Corn Silage [J]. Acta Agrestia Sinica, 2023, 31(9): 2860-2867.
[2]	LIU Yi-chao, SUN Peng-bo, ZHANG Jia-wei, LI Dong-mei, MA Hong-wei, JING Zhi-wei, WANG Rui-feng, JIA Yu-shan. Effect of Fermentation Inhibitors on Quality and Aerobic Stability of Leymus chinensis Silage [J]. Acta Agrestia Sinica, 2023, 31(8): 2564-2570.
[3]	CHEN Dan-dan, HUANG Pei-shan, ZHANG Chao, CHEN Xiao-yang, ZHOU Wei, ZHANG Qing. The Effects of Lactobacillus Plantarum on Fermentation Quality and In Vitro Digestion Characteristics of Litchi Leaves Silage [J]. Acta Agrestia Sinica, 2023, 31(7): 2232-2240.
[4]	LI Xiao, CHEN Yong-cheng, HUANG Rong-zheng, QI Ya-yin, ZHANG Fan-fan, MA Chun-hui. Effects of Compound Starter on Silage Quality and Rumen Degradation Rate of Sunflower Byproduct [J]. Acta Agrestia Sinica, 2023, 31(5): 1564-1570.
[5]	FENG Qi-xian, ZHANG Lei, LI Yan, LING Wen-qing, LI Jue, ZHOU Yi, YANG Fu-lin, ZHOU Jing. Effects of Formic Acid Treatment on the Quality and Rumen Degradation Rate of Alfalfa Silage [J]. Acta Agrestia Sinica, 2023, 31(4): 1264-1272.
[6]	CHAI Ji-kuan, ZHAO Gui-qin, JU Ze-liang. Effects of Adding Different Lactic Acid Bacteria on Oat Silage Fermentation at Low Temperatures [J]. Acta Agrestia Sinica, 2023, 31(3): 923-928.
[7]	CHEN San-dong, GUN Hai-qiang, YIN Guo-li, SHI Shang-li. Nutrient Quality and Bacterial Community Diversity of Silage Corn During Aerobic Exposure [J]. Acta Agrestia Sinica, 2023, 31(2): 388-395.
[8]	ZHAN Jia-qi, CHEN Dan-dan, GUO Tian-xin, GUO Xiang, WU Shuo, CHEN Xiao-yang, ZHOU Wei, ZHANG Qing. Effects of Different Additives on Silage Quality of Chinese Medicine Residue [J]. Acta Agrestia Sinica, 2023, 31(12): 3851-3857.
[9]	ZHENG Yang-chen, ZHAO Jun-xiang, LIU Yu-yang, BIAN Bin-bin, KONG Ling-zhi, LI Jing-chun, LI Yan-bing. Effect of Adding Polygonum persicaria to High Moisture Corn on its Bacteriostatic Ability [J]. Acta Agrestia Sinica, 2023, 31(12): 3887-3896.
[10]	BAO Wen-long, ZHENG Cheng-zhong, ZHANG Teng-wei, YUN Ying, HAN Yun-peng, LIU Zhi-ping, BA Tu, XU Guang-xiang, WANG Feng-wu, SUN Juan-juan. Effects of Nitrogen Application rate on Production Performance and Silage Quality of Whole Plant Barley [J]. Acta Agrestia Sinica, 2023, 31(10): 3167-3173.
[11]	LIU Yi-chao, SI Qiang, LIU Ming-jian, LU Xuan, YAN Meng, GE Gen-tu, WANG Zhi-jun, JIA Yu-shan. Effect of Filling Density on Quality and Aerobic Stability of Leymus chinensis and Alfalfa Silage [J]. Acta Agrestia Sinica, 2023, 31(1): 263-271.
[12]	LI Hai-ping, GUAN Hao, JIA Zhi-feng, LIU Wen-hui, CHEN Shi-yong, XU Mei-ling, CHEN Sheng-cui, YAN Dong-hai, JIANG Yong-mei, GAN Li, ZHANG Jia-min, ZHOU Qing-ping. Effects of Wheat Bran and Lactic Acid Bacteria on Baled Oat Silage Quality and Aerobic Stability in the Alpine Region of Northwest Sichuan [J]. Acta Agrestia Sinica, 2023, 31(1): 302-310.
[13]	ZHANG Jia-wei, JIA Yu-shan, GE Gen-tu, WANG Zhi-jun, BAO Jian, LI Yu-yu, PENG Dong, WANG Yu, SUN Peng-bo, YUE Xiao-ming. Effects of Different Propionic Acid Concentration on the Quality and Aerobic Stability of Natural Forage Silage [J]. Acta Agrestia Sinica, 2022, 30(9): 2522-2528.
[14]	LONG Shi-he, LI Xue-feng, PAN Jun-xin, CHANG Ye, SHI Meng-li, HUANG Nai-xin, CUI Zhi-hai, WANG Jian. Study on the Silage Quality and Microbial Community Diversity of 'Reyan No.4’ King Grass Harvested at Different Growth Days [J]. Acta Agrestia Sinica, 2022, 30(7): 1900-1908.
[15]	MA Shu-min, JIAO Ting, SHI Shang-li, QIN Wei-na, WANG Zheng-wen, ZHAO Sheng-guo, QI Juan. Effects of Mixed Lactic Acid Bacteria Preparation on Silage Quality of Different Green Corns [J]. Acta Agrestia Sinica, 2022, 30(6): 1558-1568.