Effects of Exogenous Proline Spraying on Stomatal Morphology of Reaumuria soongorica under Arid Environment

doi:10.11733/j.issn.1007-0435.2023.03.018

Acta Agrestia Sinica ›› 2023, Vol. 31 ›› Issue (3): 777-784.DOI: 10.11733/j.issn.1007-0435.2023.03.018

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Effects of Exogenous Proline Spraying on Stomatal Morphology of Reaumuria soongorica under Arid Environment

SHI Wei-ning¹, SU Shi-ping¹, LI Yi¹, ZHANG Yan², XI Jie¹

1. College of Forestry, Gansu Agricultural University, Lanzhou, Gansu Province 730070, China;
2. Tongwei County Forestry and Grassland Service Center, Dingxi, Gansu Province 743300, China

Received:2022-08-25 Revised:2022-10-23 Online:2023-03-15 Published:2023-04-01

干旱生境下外源脯氨酸对红砂气孔形态的影响

师微柠¹, 苏世平¹, 李毅¹, 张䶮², 席杰¹

1. 甘肃农业大学林学院, 甘肃兰州 730070;
2. 通渭县林业和草原服务中心, 甘肃定西 743300

通讯作者: 苏世平,E-mail:susp008@163.com
作者简介:师微柠(1998-),女,汉族,山西河津人,硕士研究生,主要从事林木种质资源保护与利用及良种快繁研究,E-mail:N13467290411@163.com
基金资助:
国家自然科学基金项目（32060335）；对发展中国家常规性科技援助项目（KY202002011）资助

Abstract

Abstract: In this study, Reaumuria soongorica was sprayed with different concentrations (0, 50, 100, 150, 200, 250 mg·L^-1) of proline (Pro) in the field environment. The 0^th day of each treatment was used as the control (CK), and stomatal characteristics (length, width, area, and density) of leaves were measured on the 1^st, 3^rd, 6^th, and 9^th day after proline spraying to reveal stomatal response to exogenous Pro. The results showed that:the stomatal characteristics of leave were significantly affected by Pro treatment, the days after Pro treatment and the interaction between Pro treatment and the days after Pro treatmen. Under each treatment, there were significant negative correlations between stomatal length and stomatal width, stomatal length and stomatal area, stomatal length and stomatal density, and significant positive correlations between other parameters. The stomatal characteristics under each Pro treatment were analyzed by line or curve fitting. It revealed there was a significant correlation between stomatal density and area, stomatal length and width, stomatal length and density (P<0.01). Therefore, exogenous Pro spraying can reduce stomata length, increase stomata width, area, and density, which significantly affected stomatal characteristics, and alleviate the adverse effects of drought stress. The concentration of 100 mg·L^-1 was the best treatment, which could significantly improve the drought resistance of R. soongorica.

Key words: Exogenous proline, Drought stress, Reaumuria soongorica, Stomatal characteristics, Correlation analysis

摘要： 本研究以大田环境内的红砂（Reaumuria soongorica）为对象，喷施不同质量浓度（0，50，100，150，200，250 mg·L^-1）的脯氨酸（Proline，Pro），以各处理的第0 d为对照（CK），测量第1，3，6，9 d的叶片气孔特征（气孔长、宽、面积和气孔密度），以揭示红砂气孔对外源Pro的响应。结果表明：在各Pro处理、作用时间和Pro处理与作用时间的交互作用下，对叶片的气孔特征都有显著影响；各处理下，气孔长与宽度、气孔面积和密度间呈极显著负相关（-0.680，-0.626，-0.690），其余指标间均达极显著正相关（0.883，0.778，0.769）；对各Pro处理下的气孔特征进行拟合，气孔密度与面积、气孔长度和宽度、气孔长度和密度间均达极显著水平（P<0.01），且具有相关性。因此，喷施外源Pro可减小红砂叶片气孔长，增大气孔宽、面积和密度，显著影响气孔特征，可缓解干旱胁迫的不利影响，其中以100 mg·L^-1浓度效果最佳，能显著提高红砂的抗旱性。

关键词: 外源脯氨酸, 干旱胁迫, 红砂, 气孔特征, 相关性分析

CLC Number:

S793.9

SHI Wei-ning, SU Shi-ping, LI Yi, ZHANG Gong, XI Jie. Effects of Exogenous Proline Spraying on Stomatal Morphology of Reaumuria soongorica under Arid Environment[J]. Acta Agrestia Sinica, 2023, 31(3): 777-784.

师微柠, 苏世平, 李毅, 张䶮, 席杰. 干旱生境下外源脯氨酸对红砂气孔形态的影响[J]. 草地学报, 2023, 31(3): 777-784.

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References

[1] ZHANASSOVA K, KURMANBAYEVA A, GADILGEREYEVA B, et al. ROS status and antioxidant enzyme activities in response to combined temperature and drought stresses in barley[J]. Acta Physiologiae Plantarum, 2021, 43(8):1-12
[2] 王园园, 赵明, 张红香, 等. 干旱胁迫对紫花苜蓿幼苗形态和生理特征的影响[J]. 中国草地学报, 2021, 43(9):78-87
[3] 王志恒, 魏玉清, 赵延蓉, 等. 基于转录组学比较研究甜高粱幼苗响应干旱和盐胁迫的生理特征[J]. 草业学报, 2022, 31(3):71-84
[4] 苏世平, 李毅, 刘小娥, 等. 外源脯氨酸对缓解红砂干旱胁迫的机理研究[J]. 草业学报, 2022, 31(6):127-138
[5] 王得运, 刘培培, 陈云婷, 等. 干旱胁迫对栀子内源激素含量的影响[J]. 中国农业科技导报, 2021, 23(4):58-63
[6] KIROVA E, PECHEVA D, SIMOVA-STOILOVA L. Drought response in winter wheat:Protection from oxidative stress and mutagenesis effect[J]. Acta Physiologiae Plantarum, 2021, 43(1):1-11
[7] AKHZARI D, GHASEMI A F. Effect of salinity and drought stress on the seedling growth and physiological traits of Vetiver Grass (Vetiveria zizanioides stapf.)[J]. Ecopersia, 2013, 1(4):339-352
[8] 孙晓梵, 张一龙, 李培英, 等. 喷施茉莉酸甲酯对干旱胁迫下狗牙根生理特性的影响[J]. 草地学报, 2022, 30(10):2811-2818
[9] LI M, WANG H, ZHAO X, et al. Role of suillus placidus in improving the drought tolerance of masson pine (Pinus massoniana Lamb.) seedlings[J]. Forests, 2021, 12(3):332
[10] 李悦, 张庆怡, 董佳伟, 等. 外源脱落酸缓解UV-B胁迫对牛皮杜鹃气孔及光合特性的影响[J]. 江苏农业科学, 2022, 50(7):145-151
[11] 许佳琦. 外源硅缓解玉米干旱胁迫的生理机制研究[D]. 哈尔滨:东北农业大学, 2021:80-87
[12] NIU J, CHEN Z, GUO Z, et al. Exogenous melatonin promotes the growth of alfalfa (Medicago sativa L.) under NaCl stress through multiple pathways[J]. Ecotoxicology and Environmental Safety, 2022(242):113938
[13] FAGHIH S, ZAMANI Z, FATAHI R, et al. Influence of kaolin application on most important fruit and leaf characteristics of two apple cultivars under sustained deficit irrigation[J]. Biological Research, 2021, 54(1):325
[14] KIJOWSKA-OBERC J, STASZAK A M, WAWRZYNIAK M K, et al. Changes in proline levels during seed development of orthodox and recalcitrant seeds of genus Acer in a climate change scenario[J]. Forests, 2020, 11(12):1362
[15] SEMIDA W M, ABDELKHALIK A, RADY M O A, et al. Exogenously applied proline enhances growth and productivity of drought stressed onion by improving photosynthetic efficiency, water use efficiency and up-regulating osmoprotectants[J]. Scientia Horticulturae, 2020(272):109580
[16] SAKAGAMI J I, HUU T N, KHUONG N Q. Foliar selenium application for improving drought tolerance of sesame (Sesamum indicum L.)[J]. Open Agriculture, 2021, 6(1):93-101
[17] AKBUDAK M A, FILIZ E. Genome-wide investigation of proline transporter (ProT) gene family in tomato:Bioinformatics and expression analyses in response to drought stress[J]. Plant Physiology and Biochemistry, 2020(157):13-22
[18] 魏斌, 李毅, 苏世平. 外源脯氨酸对自然干旱下白刺叶片气孔的影响[J]. 植物研究, 2022, 42(3):492-501
[19] 曾文静, 陈奋奇, 庄泽龙, 等. 不同外源调节物质对不同耐旱性玉米自交系的干旱缓解效应分析[J]. 干旱地区农业研究, 2020, 38(3):55-63
[20] RAGHAVENDRA A S, REDDY K B. Action of proline on stomata differs from that of abscisic acid, G-substances, or methyl jasmonate[J]. Plant Physiology, 1987, 83(4):732-734
[21] 蒋嘉瑜, 连学, 唐希明, 等. 干旱与半干旱区红砂枯落物分解初期节肢动物群落结构特征[J]. 草业学报, 2022, 31(5):156-168
[22] 李小乐, 魏亚娟, 党晓宏, 等. 红砂灌丛沙堆土壤粒度组成及养分积累特征[J]. 干旱区研究, 2022, 39(3):933-942
[23] JUBLINE B B V, PEI-FANG C, XIANG-YANG J I A, et al. Effects of foliar-spraying nitric oxide on the carbon metabolism enzymes activities and nutrients in leaves and roots of Reaumuria soongorica (pall.) Maxim seedlings under Nacl stress[J]. Plant Stress, 2022:100096
[24] 杨彪生, 单立山, 马静, 等. 红砂幼苗生长及根系形态特征对干旱-复水的响应[J]. 干旱区研究, 2021, 38(2):469-478
[25] 种培芳, 曾继娟, 单立山, 等. 干旱胁迫下荒漠草地植物红砂幼苗对外源ABA的生理响应[J]. 草地学报, 2016, 24(5):1001-1008
[26] 张玉洁, 种培芳, 贾向阳. 外源NO对干旱胁迫下红砂种子萌发特性的影响[J]. 河南农业, 2019(14):38-39
[27] 曾继娟, 种培芳, 朱强. 外源脱落酸对荒漠植物红砂幼苗光合及叶绿素荧光特性的影响[J]. 江苏农业科学, 2020, 48(7):136-141
[28] 邢宁宁. 钾、EBR和MeJA对长叶红砂响应盐和干旱胁迫的生理调控研究[D]. 呼和浩特:内蒙古大学, 2021:31-58
[29] 后有丽, 苏世平, 李毅, 等. 外源脱落酸对红砂叶片渗透调节物质含量及抗氧化酶活性的影响[J]. 草业科学, 2020, 37(2):245-255
[30] 李瑞强, 王玉祥, 孙玉兰, 等. 盐胁迫对无芒雀麦幼苗叶片形态及解剖结构的影响[J]. 草地学报, 2022, 30(6):1450-1459
[31] 王曙光, 李中青, 贾寿山, 等. 小麦叶片气孔性状与产量和抗旱性的关系[J]. 应用生态学报, 2013, 24(6):1609-1614
[32] 马丽, 单立山, 解婷婷, 等. 基于同质园实验的两种典型荒漠植物叶片功能性状变异研究[J]. 草地学报, 2022, 30(3):701-711
[33] 谢强, 陈莉. 在抗性条件下脯氨酸积累对苔藓植物的作用[J]. 现代园艺, 2020, 43(15):51-52,130
[34] 乔滨杰, 王德秋, 高海燕, 等. 干旱胁迫下杨树无性系苗期光合与气孔形态变异研究[J]. 植物研究, 2020, 40(2):177-188
[35] FERREIRA R A, BORELLA J, HUTHER C M, et al. Drought-induced stress in leaves of Coix lacryma-jobi L. under exogenous application of proline and GABA amino acids[J]. Brazilian Journal of Botany, 2020, 43(3):513-521
[36] 孙歆. 干旱下植物气孔运动调控机制研究进展[J]. 生物学教学, 2021, 46(7):24-26
[37] WANG Z, ZHENG P, MENG J, et al. Effect of exogenous 24-epibrassinolide on chlorophyll fluorescence, leaf surface morphology and cellular ultrastructure of grape seedlings (Vitis vinifera L.) under water stress[J]. Acta Physiologiae Plantarum, 2015, 37(1):1-12
[38] HAMANI A K M, LI S, CHEN J, et al. Linking exogenous foliar application of glycine betaine and stomatal characteristics with salinity stress tolerance in cotton (Gossypium hirsutum L.) seedlings[J]. BMC Plant Biology, 2021, 21(1):1-12
[39] SUN M, PENG F,XIAO Y, et al. Exogenous phosphatidylcholine treatment alleviates drought stress and maintains the integrity of root cell membranes in peach[J]. Scientia Horticulturae, 2020(259):108821
[40] 赵雅丽, 熊浩, 毕玉芬, 等. 干热胁迫对紫花苜蓿叶片气孔大小的影响[J]. 云南农业大学学报(自然科学), 2013, 28(3):336-339
[41] 薛泽民, 薛琪, 高景慧. 渗透胁迫下紫花苜蓿幼苗气孔运动与光合作用的关系[J]. 草地学报, 2018, 26(2):420-426
[42] 王凯丽, 高彦钊, 李姗, 等. 短期干旱胁迫下棉花气孔表现及光合特征研究[J]. 中国生态农业学报(中英文), 2019, 27(6):901-907
[43] 汤章城, 王育启, 吴亚华, 等. 脯氨酸对缺钾植株的保护作用[J]. 植物生理学报, 1985(2):138-146
[44] 张瀚, 李晗, 陈奇, 等. PEG模拟干旱胁迫对五唇兰光合系统的影响[J]. 热带作物学报, 2021, 42(9):2645-2652
[45] 王巧艳. 玉米副卫细胞中H₂O₂与气孔开关的关系及其来源探究[D]. 杨凌:西北农林科技大学, 2016:2-6
[46] 安国勇, 宋纯鹏, 张骁, 等. 过氧化氢对蚕豆气孔运动和质膜K⁺通道的影响[J]. 植物生理学报, 2000(5):458-464
[47] 杨凤兰, 邓冬丽, 招礼军, 等. 短叶罗汉松雌雄株叶片形态结构特征的比较研究[J]. 江西农业学报, 2021, 33(11):42-47
[48] 刘朝霞. 土壤干旱胁迫对番茄根系生长、气孔特性及保护酶活性的影响[D]. 南京:南京信息工程大学, 2016:34-42
[49] 黄荣峰, 王学巨. 气孔运动机理研究进展[J]. 应用与环境生物学报, 1996(3):320-326
[50] 朱燕华, 康宏樟, 刘春江. 植物叶片气孔性状变异的影响因素及研究方法[J]. 应用生态学报, 2011, 22(1):250-256
[51] 刘炫杉. HSL3通过调节胞内过氧化氢稳态来影响气孔关闭和干旱胁迫响应[D]. 济南:山东大学, 2021:56-64
[52] VERBRUGGEN N, HERMANS C. Proline accumulation in plants:a review[J]. Amino Acids, 2008, 35(4):753-759

Effects of Exogenous Proline Spraying on Stomatal Morphology of Reaumuria soongorica under Arid Environment

干旱生境下外源脯氨酸对红砂气孔形态的影响

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