Acta Agrestia Sinica

Acta Agrestia Sinica ›› 2023, Vol. 31 ›› Issue (3): 796-803.DOI: 10.11733/j.issn.1007-0435.2023.03.020

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The Effects of Exogenous IAA on Seed Germination and Seedling Growth of Onobrychis viciifolia Scop. under the Drought Stress

MA Xiao-lan1,2, ZHOU Hua-kun3, ZHANG Zheng-fang1,2, LI Zhen-zhen1,2, XU Meng-zhen2, HUANG Ao-wei2, WANG Wen-ying4, YIN Heng-xia1   

  1. 1. State Key Laboratory of Plateau Ecology and Agriculture Qinghai University, Xining, Qinghai Province 810016, China;
    2. College of Eco-Environmental Engineering, Qinghai University, Xining, Qinghai Province 810016, China;
    3. Key Laboratory of restoration Ecology of Cold Area in Qinghai Province, Northwest Institute of Plateau Biology Chinese Academy of Sciences, Xining, Qinghai Province 810008, China;
    4. College of life science, Qinghai Normal University, Xining, Qinghai Province 810016, China
  • Received:2022-06-17 Revised:2022-10-11 Online:2023-03-15 Published:2023-04-01

外源IAA对干旱胁迫下红豆草种子萌发及幼苗生长的影响

马小兰1,2, 周华坤3, 张正芳1,2, 李珍珍1,2, 徐梦真2, 黄奥伟2, 王文颖4, 殷恒霞1   

  1. 1. 青海大学省部共建三江源生态与高原农牧业国家重点实验室, 青海 西宁 810016;
    2. 青海大学生态环境工程学院, 青海 西宁 810016;
    3. 中国科学院西北高原生物研究所, 青海省寒区恢复生态学重点实验室, 青海 西宁 810008;
    4. 青海师范大学生命科学学院, 青海 西宁 810016
  • 通讯作者: 殷恒霞,E-mail:hengxiayin@qhu.edu.cn
  • 作者简介:马小兰(1997-),女,回族,青海海东人,硕士研究生,主要从事植物抗逆的生理和分子机制研究;E-mail:mxl2855574510@163.com
  • 基金资助:
    国家自然科学基金（31860060）；青海省科技厅应用基础研究项目（2022-ZJ-731）；国家自然科学基金联合基金项目（U21A20186）；中国科学院-青海省人民政府2020年三江源国家公园联合研究专项（LHZX-2020-08）；青海大学生态环境工程学院生态学一流学科研究生科技创新项目（2021-YX-06）；生物技术一流专业大学生科技创新项目（2021-stxy-B1和2022-stxy-B10）资助

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Abstract: To investigate the effects of exogenous IAA on the seed germination and early seedling growth of Onobrychis viciifolia under the drought stress, O. viciifolia seeds were soaked with different concentrations of indole-3-acetic acid (IAA) for 24 h;and the seeds were germinated subsequently, the seed germination, seedling phenotype, as well as physiological and biochemical characteristics were determined while treated with the drought stress. The results revealed that the seed germination of O. viciifolia was dramatically promoted under the drought stress when seeds were soaked with the 25 mg·L-1IAA, and the germination rate, germination potential, germination index and vital index of O. viciifolia seeds were optimum among four IAA treated groups with different concentrations of IAA. Furthermore, 25 mg·L-1IAA soaking treatment enhanced the primary root length, and improved the root/shoot ratio and chlorophyll content of early seedlings of O. viciifolia under the drought stress. In addition, 25 mg·L-1IAA soaking treatment was actually able to increase the activity of antioxidant enzymes and the content of osmotic regulator proline, decline the content of malondialdehyde (MDA) and hydrogen peroxide in early seedlings under the drought stress, and alleviate the oxidative damage caused by the drought stress. The high concentration of exogenous IAA (100 mg·L-1), however, inhibited the germination of seeds and the drought tolerance of early seedlings. Therefore, 25 mg·L-1 IAA soaking is helpful for the germination of O. viciifolia seeds and the drought tolerance of early seedlings, which would shed light on the efficient planting of high-quality forage O. viciifolia in arid regions.

Key words: Soaking seeds with the exogenous IAA, Drought stress, Seed germination, Seedling growth, Antioxidant enzymes

摘要： 为研究外源IAA浸种对干旱胁迫下红豆草（Onobrychis viciifolia Scop.）种子萌发及早期幼苗生长的影响，本研究利用不同浓度生长素吲哚3-乙酸（indole-3-acetic acid，IAA）浸泡处理红豆草种子24小时，测定干旱胁迫后红豆草的种子萌发、幼苗表型及其生理生化等指标。结果表明，25 mg·L-1IAA浸种处理对干旱胁迫下红豆草种子萌发具有明显的促进作用，其发芽率、发芽势、发芽指数和活力指数最大，并且能够促进主根延伸，改善根冠比和叶绿素的含量。此外，25 mg·L-1 IAA浸种处理能够提高干旱胁迫下红豆草幼苗抗氧化酶活性及渗透调节物质脯氨酸的含量，以及降低丙二醛和过氧化氢含量，缓解干旱胁迫引起的氧化损伤。但是，高浓度的外源IAA （100 mg·L-1）浸种则抑制了红豆草种子萌发及幼苗的生长发育。因此，25 mg·L-1 IAA浸种有助于红豆草种子萌发及早期幼苗的耐旱性，为优质牧草红豆草在干旱地区的高效种植提供了参考依据。

关键词: IAA浸种, 干旱胁迫, 种子萌发, 幼苗生长, 抗氧化酶

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