Abstract: The CBL-interacting protein kinase (CIPK) family is a key protein family in the Ca²⁺-mediated signaling pathway in plants. Under stresses,plants can adapt to the environmental stresses by regulating the ion transport by the CBL-CIPK network,of which CIPK24 takes a significant role. To explore the role of the CIPK gene family in Poaceae turfgrasses,Poa pratensis L. was used as the experimental material in this study. The CIPK24 gene of P. pratensis L. was cloned using RT-PCR,and its expression pattern and tissue specificity under abiotic stresses were investigated by bioinformatics and qRT-PCR. The results showed the CIPK24 from Poa pratensis L. contained a typical structural domain CIPK_C and belonged to the AMPKA_Clike superfamily,with the homology of 94.20%,90.62% and 88.62% of the CIPK24 from Lolium perenne L.,Hordeum vulgare L. and Triticum turgidum L.,respectively. The expression level of the CIPK24 gene in P. pratensis L. was tissue-specific:leaves > stems > roots. The CIPK24 gene in P. pratensis took active responses to the drought,nitrogen,phosphorus and salt stresses. Nitrogen-free,low-nitrogen,phosphorus-free and high-salt environments significantly promoted its expression. This study may provide a theoretical basis for the regulatory mechanism of the CIPK family in Poa pratensis L. under abiotic stresses.

Key words: Kentucky bluegrass, Protein Kinase, CIPK24, Abiotic stress, Expression analysis

摘要： CIPK蛋白家族是Ca²⁺介导的植物信号通路中关键蛋白家族，当植物受到逆境胁迫时，CBL-CIPK网络可调节离子运输进而适应逆境环境，其中CIPK24作用显著。为探究禾本科草坪草CIPK家族基因的作用，本研究以草地早熟禾(Poa pratensis L.)为试验材料，采用RT-PCR技术对草地早熟禾CIPK24基因进行克隆，并应用生物信息学及qRT-PCR技术，对非生物胁迫条件下该基因的表达方式及组织特异性进行研究。研究结果显示，草地早熟禾CIPK24包含典型的结构域CIPK_C，其属于AMPKA_Clike superfamily，与黑麦草(Lolium perenne L.)，大麦(Hordeum vulgare L.)，二粒小麦(Triticum turgidum L.)CIPK24同源性高达94.20%，90.62%，88.62%。草地早熟禾CIPK24基因的表达水平存在组织特异性，叶＞茎＞根。CIPK24基因积极响应干旱、氮素、磷素、盐胁迫，其中，无氮、低氮、无磷、高盐环境显著促进其表达。本研究为丰富草地早熟禾CIPK家族在非生物胁迫下的调节机制提供了理论基础。

关键词: 草地早熟禾, 蛋白激酶, CIPK24, 非生物胁迫, 表达分析