The Effects of Lactobacillus Plantarum on Fermentation Quality and In Vitro Digestion Characteristics of Litchi Leaves Silage

doi:10.11733/j.issn.1007-0435.2023.07.035

Acta Agrestia Sinica ›› 2023, Vol. 31 ›› Issue (7): 2232-2240.DOI: 10.11733/j.issn.1007-0435.2023.07.035

The Effects of Lactobacillus Plantarum on Fermentation Quality and In Vitro Digestion Characteristics of Litchi Leaves Silage

CHEN Dan-dan¹, HUANG Pei-shan¹, ZHANG Chao¹, CHEN Xiao-yang¹, ZHOU Wei¹, ZHANG Qing^1,2

1. South China Agricultural University, Guangzhou, Guangdong Province 510642, China;
2. Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Maoming, Guangdong Province 525000, China

Received:2023-04-25 Revised:2023-05-15 Online:2023-07-15 Published:2023-08-01

植物乳杆菌对荔枝叶青贮品质及体外降解特性的影响

陈丹丹¹, 黄佩珊¹, 张超¹, 陈晓阳¹, 周玮¹, 张庆^1,2

1. 华南农业大学, 广东广州 510642;
2. 岭南现代农业科学与技术广东省实验室茂名分中心, 广东茂名 525000

通讯作者: 周玮,E-mail:wzhou@scau.edu.cn;张庆,E-mail:zqing1988@126.com
作者简介:陈丹丹(1997-),女,汉族,贵州遵义人,硕士研究生,主要从事饲草加工与贮藏研究,E-mail:1744301540@qq.com
基金资助:
广东省自然基金项目(2020A1515011253；广州市科技计划项目(202102020808)；茂名实验室茂名实验室自主科研项目(2022ZD002)资助

Abstract

Abstract: Due to the restricting factor of feed shortage,making full use of natural woody plant resources as animal feed is becoming an extremely important strategy in the tropic. Using litchi leaves as an unconventional feed might meet the requirement of sustainable and intensive livestock production to reduce feed costs,avoid food competition between humans and animals,and promote feed supplies. Therefore,to investigate the feasibility of developing litchi leaves as silage,we investigated the fermentation quality of four varieties of litchi leaves ('Wanpu''Wuyejiu''Tongzai' and 'Zhuangyuanhong') ensiled with or without Lactobacillus plantarum (LP) on day 3,7,14 and 30. The in vitro dry matter digestibility (IVDMD) and accumulated gas production of litchi leaves silages were also investigated after 30 days of ensiling. The results showed that LP inclusive into the ensilage significantly reduced pH value,inhibited the coliform bacteria,and reduced the production of ammonia nitrogen of litchi leaves silage of the four varieties (P<0.01). Moreover,LP treated litchi leaves ('Wanpu' and 'Zhuangyuanhong') had lower yeasts than the untreated litchi leaves during ensiling. The number of molds in LP treated groups ('Tongzai' and 'Zhuangyuanhong') was below the detected level after 30 days ensiling,which was lower than that of the untreated groups. The addition of LP also contributed to the IVDMD improving and markedly reduction (P<0.01) of gas production of all litchi leaves silages. It was concluded in this study that LP can improve the fermentation quality and in vitro digestion characteristics of litchi leaves silage;developing litchi leaves as silage material is a feasible way to recycle litchi leaves.

Key words: Litchi leaves, Lactobacillus plantarum, Fermentation, Digestibility, Gas production

摘要： 为探究发展荔枝叶作为青贮饲料的可行性，4种荔枝叶('晚埔''乌叶舅''桶仔''状元红')分别用植物乳杆菌(Lactobacillus plantarum,LP)处理并设对照组。青贮3，7，14，30天后分析其发酵品质并测定30天后荔枝叶的干物质消化率和累积产气量。结果表明：添加LP降低了4种荔枝叶的pH值和大肠杆菌数量，同时减少了氨态氮产生(P<0.01)。此外LP处理的'晚埔'和'状元红'荔枝叶中酵母菌数量比对照低。青贮30天后，LP处理的'桶仔'和'状元红'荔枝叶中霉菌数量比对照组低，且低于检测水平。添加LP增加了4种荔枝叶的体外干物质消化率；降低(P<0.01)了产气量。本研究表明，LP添加有助于改善4荔枝叶的发酵质量以及体外发酵特性，发展荔枝叶作为青贮原料可能是回收荔枝叶的一种可行方式。

关键词: 荔枝叶, 植物乳杆菌, 发酵, 消化率, 产气量

CLC Number:

S816.5+3

CHEN Dan-dan, HUANG Pei-shan, ZHANG Chao, CHEN Xiao-yang, ZHOU Wei, ZHANG Qing. The Effects of Lactobacillus Plantarum on Fermentation Quality and In Vitro Digestion Characteristics of Litchi Leaves Silage[J]. Acta Agrestia Sinica, 2023, 31(7): 2232-2240.

陈丹丹, 黄佩珊, 张超, 陈晓阳, 周玮, 张庆. 植物乳杆菌对荔枝叶青贮品质及体外降解特性的影响[J]. 草地学报, 2023, 31(7): 2232-2240.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2023.07.035

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2023/V31/I7/2232

References

[1] WANG X,LIU H,XIE Y,et al. Effect of sucrose and lactic acid bacteria additives on fermentation quality,chemical composition and protein fractions of two typical woody forage silages[J]. Agriculture,2021,3(11):256
[2] DU Z,LIN Y,SUN L,et al. Microbial community structure,co-occurrence network and fermentation characteristics of woody plant silage[J]. Journal of the Science of Food and Agriculture,2022,3(102):1193-1204
[3] 唐丽雯,张月,岳晓琦,等. 荔枝多胺氧化酶(PAO)基因家族的全基因组鉴定与表达分析[J]. 分子植物育种,2022(19):1-17
[4] 孔祥宇. 8种生防制剂对荔枝主要病害及果实品质的影响[D]. 广州:华南农业大学,2018:1-3
[5] 苏子寒. 外源苹果多酚对荔枝采后品质及病害的影响研究[D]. 海口:海南大学,2019:1-6
[6] 徐海明,余瑶,陈炳旭,等. 荔枝叶螨的生物学特性及田间发生规律[J]. 江西农业学报,2017,29(7):67-70
[7] 温玲蓉. 荔枝叶活性成分鉴定及其生物活性研究[D]. 广州:华南理工大学,2013:1-13
[8] CASTELLAIN R C,GESSER M,TONINI F,et al. Chemical composition,antioxidant and antinociceptive properties of Litchi chinensis leaves[J]. Journal Pharm Pharmacol,2014,12(66):1796-1807
[9] MAMUN F,RAHMAN M M,ZAMILA M,et al. Polyphenolic compounds of litchi leaf augment kidney and heart functions in 2K1C rats[J]. Journal of Functional Foods,2020,64(12):103662-103662
[10] WEN L,HE J,WU D,et al. Identification of sesquilignans in litchi (Litchi chinensis Sonn.) leaf and their anticancer activities[J]. Journal of Functional Foods,2014,8(6):26-34
[11] WEN L,WU D,JIANG Y,et al. Identification of flavonoids in litchi (Litchi chinensis Sonn.) leaf and evaluation of anticancer activities[J]. Journal of Functional Foods,2014,6(6):555-563
[12] 祁金丽,梁洁,黄冬芳,等. 基于网络药理学研究荔枝叶的抗炎机制及实验验证[J]. 世界中医药,2023,18(8):1064-1071
[13] THIESEN L C,ZONTA S L,SOBRAL C R F,et al. Quality control of Litchi chinensis leaf:a potential raw material for cosmetic industry[J]. Revista Brasileira de Farmacognosia,2020,1(30):139-144
[14] 杨海,汤慧,周江江,等. 湘南地区木本饲用植物种类调查初报[J]. 衡阳师范学院学报,2021,42(6):80-85
[15] ZHANG Q,ZOU X,WU S,et al. Effects of pyroligneous acid on diversity and dynamics of antibiotic resistance genes in alfalfa silage[J]. Microbiology Spectrum,2022,4(10),0115422
[16] HISHAM M B,HASHIM A M,MOHD HANAFI N,et al. Bacterial communities associated with silage of different forage crops in Malaysian climate analysed using 16S amplicon metagenomics[J]. Scientific Reports,2022,1(12):7107
[17] WANG C,HE L,XING Y,et al. Effects of mixing Neolamarckia cadamba leaves on fermentation quality,microbial community of high moisture alfalfa and stylo silage[J]. Microbiology Biotechnology,2019,5(12):869-878
[18] TIAN J,YIN X,ZHANG J. Effects of wilting during a cloudy day and storage temperature on the fermentation quality and microbial community of Napier grass silage[J]. Journal of the Science of Food and Agriculture,2022,10(102):4384-4391
[19] CHEN D,ZHENG M,ZHOU Y,et al. Improving the quality of napier grass silage with pyroligneous acid:Fermentation,aerobic stability,and microbial communities[J]. Frontiers in Microbiology,2022(13):1034198
[20] WANG C,PIAN R,CHEN X,et al. Beneficial effects of tannic acid on the quality of bacterial communities present in high-moisture mulberry leaf and stylo silage[J]. Frontiers in Microbiology,2020(11):586412
[21] GUO X,ZHENG P,ZOU X,et al. Influence of pyroligneous acid on fermentation parameters,CO₂ production and bacterial communities of rice straw and stylo silage[J]. Frontiers in Microbiology,2021(12):701434
[22] 郑明扬,吴硕,郭香,等. 添加乳酸菌和纤维素酶对砂仁叶青贮品质的影响[J]. 草地学报,2021,29(5):1113-1117
[23] ZHOU W,PIAN R,YANG F,et al. The sustainable mitigation of ruminal methane and carbon dioxide emissions by co-ensiling corn stalk with Neolamarckia cadamba leaves for cleaner livestock production[J]. Journal of Cleaner Production,2021,29(311):127680
[24] LIU C,ZHAO G Q,WEI S N,et al. Changes in fermentation pattern and quality of Italian ryegrass (Lolium multiflorum Lam.) silage by wilting and inoculant treatments[J]. Animal Bioscience,2021,1(34):48-55
[25] FANG D,DONG Z,WANG D,et al. Evaluating the fermentation quality and bacterial community of high-moisture whole-plant quinoa silage ensiled with different additives[J]. Journal of Applied Microbiology,2022,5(132):3578-3589
[26] MUCK R E. Dry matter level effects on alfalfa silage quality I. nitrogen transformations[J] Transactions of the ASAE,1987,1(30):7-14
[27] FERREIRA G,ALFONSO M,DEPINO S,et al. Effect of planting density on nutritional quality of green-chopped corn for silage[J]. Journal of Dairy Science,2014,9(97):5918-5921
[28] LIU Y,WANG G,WU H,et al. Effect of hybrid type on fermentation and nutritional parameters of whole plant corn silage[J]. Animals,2021,6(11):1587
[29] 郭香,吴硕,郑明扬,等. 添加黄梁木叶和壳寡糖对甘蔗梢青贮饲料发酵品质及有氧稳定性的影响[J]. 草业学报,2022,31(6):202-210
[30] 吴硕,邹璇,王明亚,等. 陈皮柑汁对柱花草和水稻秸秆青贮品质的影响[J]. 草地学报,2021,29(7):1565-1570
[31] 郭香,陈德奎,陈娜,等. 含水量和添加剂对黄梁木叶青贮发酵品质的影响[J]. 草业学报,2021,30(8):199-205
[32] WANG Q,WANG R,WANG C,et al. Effects of cellulase and Lactobacillus plantarum on fermentation quality,chemical composition,and microbial community of mixed silage of whole-plant corn and peanut vines[J]. Applied Biochemistry and Biotechnology,2022,6(194):2465-2480
[33] 蔡晓蕊. 不同含水量及添加剂对燕麦和苜蓿青贮饲料营养价值及分子结构特性的影响[D]. 成都:西南民族大学,2022:24-45
[34] 侯红雁. 添加不同品种乳酸菌对半干苜蓿青贮发酵品质和体外氨基酸消化率的影响[D]. 太谷:山西农业大学,2021:45-51
[35] WANG C,PIAN R,CHEN X,ZHANG Q. Effects of polyphenol oxidases on proteolysis and lipolysis during ensiling of Moringa oleifera leaves with or without pyrocatechol[J]. Animal Feed Science and Technology,2021(275):114870
[36] WANG S,DONG Z,LI J,et al. Pediococcus acidilactici strains as silage inoculants for improving the fermentation quality,nutritive value and in vitro ruminal digestibility in different forages[J]. Journal Applied Microbiology,2019,2(126):424-434
[37] 李茂,字学娟,周汉林,等. 柱花草茎秆剪切力与营养价值动态变化和相关性[J]. 草地学报,2016,24(3):642-646
[38] GAO L,GUO X,WU S,et al. Tannin tolerance lactic acid bacteria screening and their effects on fermentation quality of stylo and soybean silages[J]. Frontiers in Microbiology,2022(13):991387
[39] 王子懿,周瑞,徐红伟,等. 香芹酚对绵羊瘤胃体外养分降解率和发酵特性及产气量的影响[J]. 甘肃农业科技,2022,53(3):50-54
[40] PAN L,HARPER K,QUEIROZ O,et al. Effects of a Bacillus based direct-fed microbial on in vitro nutrient digestibility of forage and high-starch concentrate substrates[J]. Translational Animal Science,2022,6(2):1-9
[41] ALOBA T A,DICKHOEFER U,CASRO-MONTOYA J. In vitro rumen fermentation and post-ruminal digestibility of sorghum-soybean forage as affected by ensiling length,storage temperature,and its interactions with crude protein levels[J]. Animals,2022,23(12):3400
[42] YI Q,WANG P,TANG H,et al. Fermentation quality,in vitro digestibility,and aerobic stability of ensiling spent mushroom substrate with microbial additives[J]. Animals,2023,5(13):920

The Effects of Lactobacillus Plantarum on Fermentation Quality and In Vitro Digestion Characteristics of Litchi Leaves Silage

植物乳杆菌对荔枝叶青贮品质及体外降解特性的影响

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WU Ai-li, XIAO Yu, SHAO Tao, ZONG Cheng, HUANG Zhong-yong, WU Jin-xin, LIU Qin-hua, RONG Hui. Investigation on β-Carotene Loss Inhibition Mechanism by Silage Fermentation Products-Squalene [J]. Acta Agrestia Sinica, 2023, 31(7): 1988-1995.
[2]	HUANG Pei-shan, ZHANG Chao, CHEN Dan-dan, ZHANG Qing. Effect of Leaf Extracts of Moringa oleracea on the Quality of Stylosanthes guianensis and Pennisetum purpureum Silages [J]. Acta Agrestia Sinica, 2023, 31(7): 2194-2202.
[3]	CHEN Ting, LIU Yue, SUN Rong, ZI Xue-juan, LYU Ren-long, LI Mao. Assessment onthe Feeding Value of Plant Waste of 21Tropical Garden Species [J]. Acta Agrestia Sinica, 2023, 31(5): 1414-1424.
[4]	LI Xiao, CHEN Yong-cheng, HUANG Rong-zheng, QI Ya-yin, ZHANG Fan-fan, MA Chun-hui. Effects of Compound Starter on Silage Quality and Rumen Degradation Rate of Sunflower Byproduct [J]. Acta Agrestia Sinica, 2023, 31(5): 1564-1570.
[5]	CHAI Ji-kuan, ZHAO Gui-qin, JU Ze-liang. Effects of Adding Different Lactic Acid Bacteria on Oat Silage Fermentation at Low Temperatures [J]. Acta Agrestia Sinica, 2023, 31(3): 923-928.
[6]	LI Hai-ping, GUAN Hao, JIA Zhi-feng, LIU Wen-hui, CHEN Shi-yong, XU Mei-ling, CHEN Sheng-cui, YAN Dong-hai, JIANG Yong-mei, GAN Li, ZHANG Jia-min, ZHOU Qing-ping. Effects of Wheat Bran and Lactic Acid Bacteria on Baled Oat Silage Quality and Aerobic Stability in the Alpine Region of Northwest Sichuan [J]. Acta Agrestia Sinica, 2023, 31(1): 302-310.
[7]	ZHANG Jia-wei, JIA Yu-shan, GE Gen-tu, WANG Zhi-jun, BAO Jian, LI Yu-yu, PENG Dong, WANG Yu, SUN Peng-bo, YUE Xiao-ming. Effects of Different Propionic Acid Concentration on the Quality and Aerobic Stability of Natural Forage Silage [J]. Acta Agrestia Sinica, 2022, 30(9): 2522-2528.
[8]	ZHOU Ya-nan, XIA Hong-ze, LIU Shu-jie, SUN Lu, CUI Zhan-hong, ZHOU Lei, ZHOU Yu-qing. Effects of Different CBI Diets on Rumen Fermentation Parameters,Serum Biochemical Indices and Nutrient Digestibility of Yak [J]. Acta Agrestia Sinica, 2022, 30(8): 2217-2222.
[9]	LONG Shi-he, LI Xue-feng, PAN Jun-xin, CHANG Ye, SHI Meng-li, HUANG Nai-xin, CUI Zhi-hai, WANG Jian. Study on the Silage Quality and Microbial Community Diversity of 'Reyan No.4’ King Grass Harvested at Different Growth Days [J]. Acta Agrestia Sinica, 2022, 30(7): 1900-1908.
[10]	ZHANG Dan-dan, ZHANG Yuan-qing, LIANG Yuan, CHENG Jing, WANG Dong-cai, LI Bo, SUN Rui-feng, JIN Guang, XU Fang. Evaluation of the Associative Effects of Concentrate、Broussonetia papyrifera and Whole Plant Corn Silage based on Principal Component Analysis [J]. Acta Agrestia Sinica, 2022, 30(7): 1909-1917.
[11]	MA Shu-min, JIAO Ting, SHI Shang-li, QIN Wei-na, WANG Zheng-wen, ZHAO Sheng-guo, QI Juan. Effects of Mixed Lactic Acid Bacteria Preparation on Silage Quality of Different Green Corns [J]. Acta Agrestia Sinica, 2022, 30(6): 1558-1568.
[12]	JIA Hong-ding, HUANG Li-juan, LIU Lei, SUN Rong-ji, ZHANG Zhi-fei, CHEN Gui-hua. Comparative Study on the Quality of Rice Straw Silage of Different Varieties [J]. Acta Agrestia Sinica, 2022, 30(5): 1310-1318.
[13]	ZHAO Mu-qier, WANG Zhi-jun, BAO Jian, SUN Lin, ZHANG Yong-hu, WEN Rui, WANG Wei, JIA Yu-shan, GE Gen-tu. Effect of Different Silage Additives on the Silage Quality of Forage Millet [J]. Acta Agrestia Sinica, 2022, 30(4): 1021-1026.
[14]	FU Dong-qing, JIA Chun-ying, ZHANG Li, ZHANG Fan-fan, MA Chun-hui. Dynamic Analysis and Evaluation of Agronomic Traits and Fermentation Quality of Maize Silage in Shihezi Region [J]. Acta Agrestia Sinica, 2022, 30(3): 682-691.
[15]	FU Wei, CHEN Wei, ZHOU Li, CAI Lu, WANG Xiao-li, HAN Yong-fen, MENG Jun-jiang. Effects of Mixing Ratio of Lactobacillus plantarum and L.brevis on Sorghum Hybrid Sudan Grass Silage [J]. Acta Agrestia Sinica, 2022, 30(3): 758-763.