Effects of Phlorotannins extract on Rumen Fermentation Performance and Major Rumen Bacteria of Dairy Cow

doi:10.11733/j.issn.1007-0435.2023.08.010

Acta Agrestia Sinica ›› 2023, Vol. 31 ›› Issue (8): 2343-2351.DOI: 10.11733/j.issn.1007-0435.2023.08.010

Previous Articles Next Articles

Effects of Phlorotannins extract on Rumen Fermentation Performance and Major Rumen Bacteria of Dairy Cow

CHEN Yu-hua, WEI Yuan-hao, JI Hui-min, HUANG Qian-qian

Yangzhou University, Yangzhou, Jiangsu Province 225000, China

Received:2023-02-06 Revised:2023-03-17 Online:2023-08-15 Published:2023-09-01

褐藻多酚提取物对奶牛瘤胃发酵性能及主要瘤胃细菌含量的影响

陈誉华, 魏元浩, 吉慧敏, 黄倩倩

扬州大学, 江苏扬州 225000

通讯作者: 黄倩倩,E-mail:huangq0315@126.com
作者简介:陈誉华(1996-),女,汉族,江苏南通人,硕士研究生,从事植物提取物的开发与利用研究,E-mail:Ovaltine0930@163.com
基金资助:
财政部和农业农村部：国家现代农业产业技术体系(CARS36)；国家自然科学基金(31902191)共同资助

Abstract

Abstract: This study aimed to investigate the effects of Phlorotannins extract on in vitro rumen fermentation and main rumen bacteria in dairy cows. The experiment used rice straw:soybean meal (6∶4) as a fermentation substrate apportioned into eight treatment groups,namely 0,125,250,and 500 μg·mL^-1 phlorotannin (PT) supplements and 30 mg polyethylene glycol (PEG) supplementation to the all individual phlorotannin (PT) supplements. The results showed that: The addition of phlorotannins extract significantly decreased pH value and ammonia nitrogen concentration (P<0.05) in rumen substances,promoted the transformation of rumen fermentation type,increased Prevotella ruminicola and Streptococcus bovis,and decreased Methanobrevibacter ruminantium (P<0.05). The MFAEI of 125 μg·mL^-1 supplement was the highest in all rumen fermentation parameters,regardless of PEG addition or not. In conclusion,dietary supplement of phlorotannins extract provided a more acidic environment for rumen fermentation,consequently to reduce the protein decomposition,and potentially decrease methane emissions. In this experiment,the optimal dosage of phlorotannins supplement was 125 μg·mL^-1.

Key words: Phlorotannins, Rumen fermentation, Dry matter degradation rate, Rumen bacteria

摘要： 本文旨在探究添加褐藻多酚提取物对奶牛瘤胃体外发酵和主要瘤胃细菌含量的影响。以水稻(Oryza sativa L.)秸秆∶豆(Glycine max)粕=6∶4为发酵底物，试验共分9组，前4组在底物的基础上分别添加0,125,250,500 μg·mL^-1褐藻多酚(Phlorotannin，PT)；后4组在前4组的基础上添加30 mg聚乙二醇(PEG)。结果表明：褐藻多酚提取物的添加能够显著降低瘤胃pH值和氨态氮浓度(P＜0.05)，促进瘤胃发酵类型的转变，显著提高了普雷沃氏菌和牛链球菌含量，降低瘤胃甲烷短杆菌的含量(P＜0.05)；对瘤胃发酵各项参数进行综合评定，无论是否添加PEG，125 μg·mL^-1组的多项组合效应值(MFAEI)均最高。综上所述，日粮中添加褐藻多酚提取物为瘤胃发酵提供了一个更具酸性的环境，减少了瘤胃内蛋白质的分解，具有降低甲烷排放的潜能，且在本试验中褐藻多酚的最佳添加量为125 μg·mL^-1。

关键词: 褐藻多酚, 瘤胃发酵, 干物质降解率, 瘤胃细菌

CLC Number:

S816.79

CHEN Yu-hua, WEI Yuan-hao, JI Hui-min, HUANG Qian-qian. Effects of Phlorotannins extract on Rumen Fermentation Performance and Major Rumen Bacteria of Dairy Cow[J]. Acta Agrestia Sinica, 2023, 31(8): 2343-2351.

陈誉华, 魏元浩, 吉慧敏, 黄倩倩. 褐藻多酚提取物对奶牛瘤胃发酵性能及主要瘤胃细菌含量的影响[J]. 草地学报, 2023, 31(8): 2343-2351.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2023.08.010

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2023/V31/I8/2343

References

[1] CORNESCU G M,VLAICU A P,UNTEA A E,et al. The effects of diets incorporating natural source of tannins on laying hens' production performances and physical parameters of eggs[J]. Archiva Zootechnica,2022,25(2):75-85
[2] CHOI J H,LIU G C,GOO D Y,et al. Effects of tannic acid supplementation on growth performance,gut health,and meat production and quality of broiler chickens raised in floor pens for 42 days[J]. Frontiers in Physiology,2022(13):1082009
[3] WANG Z,ZHAO Y,LAN X Y,et al. Tannic acid supplementation in the diet of Holstein bulls:Impacts on production performance,physiological and immunological characteristics,and ruminal microbiota[J]. Frontiers in Nutrition,2022(9):1066074
[4] MOHAMMADABADI T,NORUZI H N,HOSEINI S,et al. Effect of dietary Conocarpus erectus leaves and branches on milk yield,quality,antioxidant activity and fatty acid profile,and blood parameters of Najdi dairy goats[J]. Translational Animal Science,2023,7(1):1-7
[5] VARGAS-ORTIZ L,CHAVEZ-GARCIA D,BARROS-RODRíGUEZ M,et al. Rumen function and in vitro gas production of diets influenced by two levels of tannin-rich forage[J]. Fermentation,2022,8(11):607
[6] LAZZARI G,MüENGER A,HEIMO D,et al. Effects of tanniferous sainfoin and Acacia mearnsii extract on urinary N excretion and ammonia volatilization from the slurry of dairy cows[J]. Animal Feed Science and Technology,2023,297(5):115577
[7] DOMINGUEZ H. Functional ingredients from algae for foods and nutraceuticals[M]. Woodhead Publishing,Cambridge CB22 3HJ,UK,2013,ISBN 9780857095121
[8] MUELLER-HARVEY I. Unravelling the conundrum of tannins in animal nutrition and health[J]. Journal of the Science of Food and Agriculture,2006,86(13):2010-2037
[9] 何袅袅,陈雅鑫,蔡树芸,等.海藻多酚提取分离及生物活性研究进展[J].食品工业,2022,43(10):190-194
[10] 王黎,王传杰,黄倩倩.褐藻多酚在动物营养中的应用及研究进展[J].当代畜牧,2021,474(8):19-22
[11] 郭晓华,谢正军,吴志青,等.海藻提取物对育肥猪生长性能、免疫功能和肉品质的影响[J].饲料工业,2022,43(23):20-23
[12] 苏国旗,秦林林,周晓容,等.海藻提取物在猪和鸡生产中的应用研究进展[J].中国畜牧杂志,2022,58(1):66-70,76
[13] WANG Y,XU Z,BACH S J,et al. Effects of phlorotannins from Ascophyllum nodosum (brown seaweed) on in vitro ruminal digestion of mixed forage or barley grain[J]. Animal Feed Science and Technology,2008(145):375-395
[14] MENKE K H,STEINGASS H. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid[J]. Animal Research and Development,1988(28):7-55
[15] BOUCHAREB R,DERBAL K,BENALIA A. Optimization of active coagulant agent extraction method from Moringa oleifera seed for municipal wastewater treatment[J]. Water Science and Technology,2021(84):393-403
[16] LWIN K O,HAYAKAWA M,BAN-TOKUDA T,et al. Real-time PCR assays for monitoring anaerobic fungal biomass and population size in the rumen[J]. Current Microbiology,2011,62(4):1147-1151
[17] DENMAN S E,TOMKINS N W,MCSWEENEY C S. Quantitation and diversity analysis of ruminal methanogenic populations in response to the antimethanogenic compound bromochloromethane[J]. FEMS Microbiology Ecology,2007,62(3):313-322
[18] WANG R F,CAO W W,CERNIGLIA C E. PCR detection of Ruminococcus spp. in human and animal faecal samples[J]. Molecular and Cellular Probes,1997,11(4):259-265
[19] LI M,PENNER G B,HERNANDEZ-SANABRIA E,et al. Effects of sampling location and time,and host animal on assessment of bacterial diversity and fermentation parameters in the bovine rumen[J]. Journal of Applied Microbiology,2010,107(6):1924-1934
[20] MAO H,XIA Y,YAN T,et al. Effects of various weaning times on growth performance,rumen fermentation and microbial population of yellow cattle calves[J]. Asian-Australasian Journal of Animal Sciences,2017,30(11):1557-1562
[21] 魏德泳,朱伟云,毛胜勇.日粮不同NFC/NDF比对山羊瘤胃发酵与瘤胃微生物区系结构的影响[J].中国畜牧兽医文摘,2012,28(8):216
[22] 武瑞兵,苏蕊,张燕军,等.Real-time PCR法检测日粮中添加小黑麦干酒糟对肉牛瘤胃Prevotella属菌数量的影响[J].微生物学报,2010,37(12):1760-1766
[23] SATO Y,TAKEBE H,TOMINAGA K,et al. Taxonomic and functional characterization of the rumen microbiome of Japanese Black cattle revealed by 16S rRNA gene amplicon and metagenome shotgun sequencing[J]. FEMS Microbiology Ecology,2021,97(12):52
[24] HATAMI A,ALIPOUR D,HOZHABRI F,et al. Effect of different levels of pomegranate marc with or without polyethylene glycol on performance,nutrients digestibility and protozoal population in growing lambs[J]. Animal Feed Science and Technology,2018(235):15-22
[25] ADELUSI O O,ADERINBOYE S R Y,ADEDARA S T,et al. Inclusion of Bullock leaves decreased the in vitro total gas and methane production of cassava-rich diet[J]. Animal-Science Proceedings,2022,13(1):17-18
[26] CHUZAEMI S,MASHUDI M,H ERYANTRISTAN,et al. Effect of pollard and soybean meal protected with condensed tannin in concentrate on in vitro gas production[J]. IOP Conference Series:Earth and Environmental Science,2020,478(1):012053
[27] 丛树发,李伟,丛硕,等.湿贮玉米体外发酵pH值、总产气量及菌体蛋白变化的研究[J].现代畜牧兽医,2022,409(12):24-27
[28] 王敬尧.单宁对绵羊瘤胃发酵参数,营养物质消化率和甲烷排放量的影响[D].呼和浩特:内蒙古农业大学,2019:50-52
[29] 沈思聪,罗佳璇,魏元浩,等.体外产气法研究百脉根及其缩合单宁对瘤胃发酵特性及降解率的影响[J].饲料工业,2020,41(17):22-26
[30] 肖敏敏,解彪,杨潇,等.高粱单宁提取物与聚乙二醇对体外瘤胃发酵参数及营养物质消化率的影响[J].动物营养学报,2021,33(3):1576-1583
[31] 陈雷,暴雪艳,郭刚,等.单宁酸和乳酸菌对紫花苜蓿青贮品质和体外瘤胃发酵的影响[J].草地学报,2021,29(8):1853-1858
[32] SARNATARO C,SPANGHERO M. In vitro rumen fermentation of feed substrates added with chestnut tannins or an extract from Stevia rebaudiana Bertoni[J]. Animal Nutrition,2020,6(1):54-60
[33] 王敬尧,解湧芳,丽丽,等.添加不同水平单宁酸对绵羊体外发酵参数和甲烷产量的影响[J].中国畜牧兽医,2020,47(5):1436-1443
[34] 张海铉,霍文婕,陈雷,等.添加剂对紫花苜蓿青贮品质及小肠可吸收氨基酸的影响[J].草地学报,2022,30(5):1302-1309
[35] 曹雪,黄小乘,王宣力,等.不同氮源添加低水平缩合单宁对延边黄牛瘤胃发酵特性及降解率的影响[J].中国畜牧兽医,2021,48(8):2809-2819
[36] 沈冰蕾,苗树君,曲永利,等.不同种类异位酸添加水平对奶牛瘤胃体外发酵的影响[J].中国兽医学报,2013,33(7):1086-1090
[37] 魏欢,李翔宇,于全平,等.添加5种植物酚类化合物对高精料底物瘤胃体外发酵及产甲烷的影响[J].草业学报,2018,27(11):192-199
[38] 张振东,刘婷,范慧玉,等.单宁酸对湖羊瘤胃动态发酵和血清抗氧化能力的影响[J].饲料工业,2022,43(18):44-49
[39] YEOMAN C J,FOELDS C J,LEPERCQ P,et al. In vivo competitions between Fibrobacter succinogenes,Ruminococcus flavefaciens,and Ruminoccus albus in a gnotobiotic sheep model revealed by multi-omic analyses[J]. mBio,2021,12(2):03533-20
[40] PATRA A K,SAXENA J. Exploitation of dietary tannins to improve rumen metabolism and ruminant nutrition[J]. Journal of the Science of Food and Agriculture,2011,91(1):24-37
[41] BAE H D,MCALLISTER T A,YANKE J,et al. Effects of condensed tannins on endoglucanase activity and filter paper digestion by Fibrobacter succinogenes S85[J]. Applied and Environmental Microbiology,1993,59(7):2132-2138
[42] 刘鑫,姜鑫,李洋,等. 瘤胃微生物对甲烷生成的互作效应及减排措施[C]//.中国畜牧协会.第十二届中国牛业发展大会论文集.遵义:[出版者不详],2017:158-163
[43] YANG J,HAO Y,LI N,et al. Metabolic and microbial modulation of phenolic compounds from raspberry leaf extract under in vitro digestion and fermentation[J]. International Journal of Food Science and Technology,2021,56(10):5168-5177
[44] 于鸿玲,李艳飞,逄晓阳,等.瘤胃反刍月形单胞菌的分离鉴定[J].中国兽医杂志,2006(11):25-26
[45] WANG Y X,T W ALEXANDER,T A MCALLISTER. In vitro effects of phlorotannins from Ascophyllum nodosum (brown seaweed) on rumen bacterial populations and fermentation[J]. Journal of the Science of Food and Agriculture,2009,89(13):2253-2260
[46] 金巍.草食动物厌氧真菌及其共存甲烷菌的分离鉴定和体外发酵特性的初步研究[D].南京:南京农业大学,2009:64-85
[47] 黄祥元,唐伟,龙冰雁.金银花提取物对肉牛瘤胃体外发酵、挥发性脂肪酸组成及气体产生的影响[J].中国饲料,2019(24):55-58

Effects of Phlorotannins extract on Rumen Fermentation Performance and Major Rumen Bacteria of Dairy Cow

褐藻多酚提取物对奶牛瘤胃发酵性能及主要瘤胃细菌含量的影响

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 7

Recommended Articles

Metrics

Comments

[1]	ZHOU Ya-nan, XIA Hong-ze, LIU Shu-jie, SUN Lu, CUI Zhan-hong, ZHOU Lei, ZHOU Yu-qing. Effects of Different CBI Diets on Rumen Fermentation Parameters,Serum Biochemical Indices and Nutrient Digestibility of Yak [J]. Acta Agrestia Sinica, 2022, 30(8): 2217-2222.
[2]	ZHANG Dan-dan, ZHANG Yuan-qing, LIANG Yuan, CHENG Jing, WANG Dong-cai, LI Bo, SUN Rui-feng, JIN Guang, XU Fang. Evaluation of the Associative Effects of Concentrate、Broussonetia papyrifera and Whole Plant Corn Silage based on Principal Component Analysis [J]. Acta Agrestia Sinica, 2022, 30(7): 1909-1917.
[3]	YAO Xi-xi, CHEN Yong-long, XU Cheng-ti, HAI Cun-xiu, LIU Hao-dong. Effects of Dietary Energy Level on Production Performance,Meat Quality and Rumen Microbes of Yak (Bos grunniens) [J]. Acta Agrestia Sinica, 2022, 30(2): 432-439.
[4]	YAO Xi-xi, CAI Hua, LIU Hao-dong, LI Chang-hui. Analysis of Rumen Fermentation Characteristics and In Vitro Digestibility of Alpine Meadow Forage in Different Seasons [J]. Acta Agrestia Sinica, 2021, 29(8): 1729-1737.
[5]	CHEN Lei, BAO Xue-yan, GUO Gang, HUO Wen-jie, LI Qing-hong, XU Qing-fang, WANG Cong, LIU Qiang. Effects of Tannic Acid and Lactic Acid Bacteria on Alfalfa Silage Quality and Ruminal Fermentation in Vitro [J]. Acta Agrestia Sinica, 2021, 29(8): 1853-1858.
[6]	XIA Hong-ze, ZHANG Lin-lin, WANG Yin-meng, SUN Lu, Wang Lei, LIU Shu-jie, CUI Zhan-hong, LIU Ming-lian. Evaluation of Fermentation Characteristics of Different Grading Indexes Corn Silage and Alfalfa Hay Combination Using in Vitro Gas Production [J]. Acta Agrestia Sinica, 2020, 28(4): 1136-1144.
[7]	Yisilayi·Dawuti, AN Sha-zhou, Ainiwaer·Aishan, Abuduwayiti·Sadike, Reziya·Mutailifu. A Study on the Effect of Different Drying Methods on Hay Quality of Different Stubbles of Sudangrass [J]. Acta Agrestia Sinica, 2016, 24(3): 663-668.