The Correlation Between Gene Expression Induced by Low-Temperature Stress and Key Agronomic Traits of Alfalfa

doi:10.11733/j.issn.1007-0435.2024.11.003

Acta Agrestia Sinica ›› 2024, Vol. 32 ›› Issue (11): 3358-3370.DOI: 10.11733/j.issn.1007-0435.2024.11.003

The Correlation Between Gene Expression Induced by Low-Temperature Stress and Key Agronomic Traits of Alfalfa

PENG Jia-wen, XIA Hou-yin, MA Zhi-chao, CUI Yi-lin, YUAN Wang, MIAO Yu-qi, TONG Yu-tao, WU Jia-xin, ZHANG Meng-yao, YUAN Zi-ang, GAO Jing-hui, YANG Pei-zhi

College of Grass and Grassland, Northwest A & F University, Yangling, Shaanxi Province 712100, China

Received:2024-02-23 Revised:2024-05-05 Published:2024-11-29

低温诱导基因表达与紫花苜蓿主要农艺性状间的关系

彭嘉文, 夏厚胤, 马志超, 崔祎琳, 苑旺, 苗愉祺, 同雨涛, 武佳昕, 张梦瑶, 袁子昂, 高景慧, 杨培志

西北农林科技大学草业与草原学院, 陕西杨陵 712100

通讯作者: 高景慧,E-mail:gaojinghui@nwsuaf.edu.cn;杨培志,E-mail:yangpeizhi@126.com
作者简介:彭嘉文(2000-),女,汉族,河南南阳人,硕士研究生,主要从事遗传资源与牧草育种研究,E-mail:pengjiawen2021@nwsuaf.edu.cn;夏厚胤(2000-),男,汉族,湖北十堰人,硕士研究生,主要从事遗传资源与牧草育种研究,E-mail:xiahy27@163.com
基金资助:
财政部和农业农村部：国家现代农业产业技术体系资助(CARS-34)，陕西省重点研发一般项目(2019 NY-074)资助

Abstract

Abstract: Alfalfa (Medicago sativa L.) is a widely cultivated forage worldwide,while the growth and production of alfalfa in cold northern regions are primarily limited by low-temperature stress. The present study aimed to investigate the integration of cold resistance identification and screening for high-yield candidate varieties during the early selection phase. Four varieties of alfalfa as experimental materials were used to establish T₁ mutant populations through ethyl methanesulfonate (EMS,C₃H₈O₃S) mutagenesis. By combining this with the selection of exceptional mutants exhibiting high-yield agronomic traits,we sought to explore the relationship between gene expression levels induced by low temperature and major agronomic traits associated with high yield. The research here revealed that main agronomic characteristics of high-quality and cold-resistant mutant alfalfa include tall plants,robust stems,and abundant branches and leaves. And low-temperature stress induced high expression of MsG0180003510 (HIPP),MsG0880047253 (LEA),MsG0880047531 (TPS),and MsG0180005121 (PM),while their expression levels exhibited major roles according to membership function analysis. Specifically,HIPP showed a significant positive correlation with stem diameter (P<0.05),while LEA demonstrated significant positive correlations with stem diameter but negative correlations with branch number and internode number (P<0.05). PM exhibited significant positive correlations with stem diameter,branch number,and dry/fresh weight (P<0.05). The study reveals the correlation between low-temperature-induced gene expression and agronomic traits in alfalfa;and suggests that the combination of tall plants with robust stems and abundant branches and leaves,along with the high expression of low-temperature-induced genes HIPP,LEA,TPS and PM,can be used as a predictor for selecting high-yield and cold-resistant alfalfa varieties during early growth stages,providing a foundation for survival strategies under low temperature stress.

Key words: Alfalfa, Low-temperature stress, Agronomic traits, Low-temperature-induced genes

摘要： 低温限制紫花苜蓿(Medicago sativa L.)生长发育，严重影响其产量。为在作物生长早期筛选高产抗寒候选品种，本研究以4种紫花苜蓿为试验材料，利用甲基磺酸乙酯(Ethyl methanesulfonate，EMS)诱变建立T₁突变群体，结合优良突变体高产相关农艺性状评价，拟探索低温诱导基因表达量与高产农艺性状间的关系。结果表明：优质抗寒苜蓿突变体植株表现为植株高大、茎秆粗壮、分枝数多、叶面积大，同时低温诱导基因MsG0180003510 (HIPP)，MsG0880047253(LEA)，MsG0880047531(TPS)和MsG0180005121(PM)表达水平在隶属函数分析中排位最高；其中HIPP与茎粗显著正相关(P＜0.05)，LEA与茎粗显著正相关、与分枝数和节间显著负相关(P＜0.05)，PM和茎粗、分枝数和干鲜重显著正相关(P＜0.05)。研究指出苜蓿中农艺性状表现为茎秆粗壮、枝繁叶茂，且分子水平HIPP，LEA，TPS和PM基因高表达，可作为在生长早期筛选高产耐寒苜蓿品种的预测因子，为紫花苜蓿在低温胁迫下的生存策略提供依据。

关键词: 紫花苜蓿, 低温胁迫, 农艺性状, 低温诱导基因

CLC Number:

S542

PENG Jia-wen, XIA Hou-yin, MA Zhi-chao, CUI Yi-lin, YUAN Wang, MIAO Yu-qi, TONG Yu-tao, WU Jia-xin, ZHANG Meng-yao, YUAN Zi-ang, GAO Jing-hui, YANG Pei-zhi. The Correlation Between Gene Expression Induced by Low-Temperature Stress and Key Agronomic Traits of Alfalfa[J]. Acta Agrestia Sinica, 2024, 32(11): 3358-3370.

彭嘉文, 夏厚胤, 马志超, 崔祎琳, 苑旺, 苗愉祺, 同雨涛, 武佳昕, 张梦瑶, 袁子昂, 高景慧, 杨培志. 低温诱导基因表达与紫花苜蓿主要农艺性状间的关系[J]. 草地学报, 2024, 32(11): 3358-3370.

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The Correlation Between Gene Expression Induced by Low-Temperature Stress and Key Agronomic Traits of Alfalfa

低温诱导基因表达与紫花苜蓿主要农艺性状间的关系

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