Acta Agrestia Sinica ›› 2024, Vol. 32 ›› Issue (2): 535-542.DOI: 10.11733/j.issn.1007-0435.2024.02.022

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Effects of Melatonin on Seed Germination and Antioxidant Physiology of Alfalfa Seedlings Under Aluminum Stress

LI Qi-jiao1, CUI Dong-yi2, DUAN Xin-hui1, LIU Li1, XU Wen-hua1, LI Qing-xi1, ZHENG Li-wen1, REN Jian1, ZHU Li-min1, MA Xiang-li1   

  1. 1. Yunnan Agricultural University Yunnan, Kunming, Yunnan Province 650201, China;
    2. Diqing Academy of Animal Husbandry and Veterinary Science, Shangri-La, Yunnan Province 674400, China
  • Received:2023-06-03 Revised:2023-08-15 Online:2024-02-15 Published:2024-03-06

褪黑素浸种对铝胁迫下苜蓿种子萌发及幼苗抗氧化生理的影响

李启娇1, 崔东毅2, 段新慧1, 刘莉1, 许文花1, 李庆玺1, 郑立文1, 任健1, 朱丽敏1, 马向丽1   

  1. 1. 云南农业大学, 云南 昆明 650201;
    2. 迪庆州畜牧兽医科学研究院, 云南 香格里拉 674400
  • 通讯作者: 马向丽,E-mail:xfmaxiangli@126.com
  • 作者简介:李启娇(1999-),女,汉族,云南昆明人,硕士研究生,主要从事牧草资源及育种研究,E-mail:1789181285@qq.com
  • 基金资助:
    云南省农业基础研究联合专项(202301BD070001-163);洱源县饲草产业科技特派团(202304BI090008)资助

Abstract: To investigate the effects of exogenous melatonin (MT) solution on seed germination and seedling growth of Medicago sativa L. under aluminum stress,‘WL525HQ’ (aluminum resistant) and ‘Suntory’ (aluminum sensitive) alfalfa were used as materials. The seeds were soaked with MT solution of different concentrations (0,100,200,400,800 μmol·L-1),and the seed germination,seedling growth,and antioxidant physiology of seedlings were measured under aluminum stress (1 mmol·L-1). The results showed that aluminum stress inhibited the seed germination and seedling growth of alfalfa. MT seed soaking alleviated the oxidative damage caused by aluminum stress by increasing the activity of antioxidant enzymes and reducing the accumulation of H2O2 and MDA. With the increase of MT solution concentration,alfalfa seed germination rate,germination potential,root length,plant height,biomass and root antioxidant enzyme activity increased first and then decreased,while H2O2 and MDA contents decreased first and then increased. The variation range of ‘WL525HQ’ was higher than that of ‘Suntory’. The effects of different concentrations of MT on alfalfa aluminum toxicity were evaluated by combining germination indexes,growth indexes,antioxidant enzyme activities,H2O2 and MDA contents. The results showed that 200 μmol·L-1 treatment had the best effect,400 μmol·L-1 was the second,and 800 μmol·L-1 was the worst.

Key words: Melatonin, Alfalfa, Aluminum stress, Seed germination, Seedling growth

摘要: 为探明外源褪黑素(Melatonin,MT)溶液浸种对铝胁迫下紫花苜蓿(Medicago sativa L.)种子萌发及幼苗生长的影响,本试验以‘WL525HQ’(耐铝)和‘三得利’(敏铝)紫花苜蓿为材料,使用不同浓度MT溶液(0,100,200,400,800 μmol·L-1)浸种,测定铝胁迫(1 mmol·L-1)下种子萌发、幼苗生长、幼苗抗氧化生理等指标。结果表明,铝胁迫抑制了紫花苜蓿种子萌发及幼苗生长,MT浸种通过提高抗氧化酶活性、减少H2O2和丙二醛的积累来减轻铝胁迫给紫花苜蓿带来的氧化伤害;且随着MT溶液浓度的增加,紫花苜蓿种子发芽率、发芽势、根长、株高、生物量及根系抗氧化酶活性呈先升后降趋势,H2O2和丙二醛含量呈先降后升趋势,‘WL525HQ’的变化幅度高于‘三得利’。综合发芽指标、生长指标、抗氧化酶活性、H2O2和丙二醛含量评价不同浓度MT对苜蓿铝毒害的缓解效果表明:200 μmol·L-1处理效果较好,400 μmol·L-1次之,800 μmol·L-1最差。

关键词: 褪黑素, 紫花苜蓿, 铝胁迫, 种子萌发, 幼苗生长

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