Effects of Forest Fire Intensity on Functional Genes of Soil Nitrogen Cycling in Larix Gmelinii

doi:10.11733/j.issn.1007-0435.2024.03.008

Acta Agrestia Sinica ›› 2024, Vol. 32 ›› Issue (3): 726-735.DOI: 10.11733/j.issn.1007-0435.2024.03.008

Effects of Forest Fire Intensity on Functional Genes of Soil Nitrogen Cycling in Larix Gmelinii

SHU Yang^1,2, CHEN Jin-ping⁵, DING Zhao-hua⁵, LI Hang^3,4, ZHAO Peng-wu^1,2, ZHOU Mei^1,2, JIA Wen-jie^1,2, ZHANG Yi-chao^1,2, ZHANG Guo-chen⁶, WEI Jiang-sheng^2,3,4

1. Forestry College of Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010019, China;
2. Saihanwula Forest Ecosystem National Station, Chifeng, Inner Mongolia 025150, China;
3. College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010011, China;
4. Inner Mongolia Key Laboratory of Soil Quality and Nutrient Resource, Hohhot, Inner Mongolia 010011, China;
5. Inner Mongolia Genhe Forest Industry Co. LTD, Genhe, Inner Mongolia 022350, China;
6. Huamugou Forestry, Keshiketeng Banner, Chifeng City, Inner Mongolia, China, Chifeng, Inner Mongolia 025350, China

Received:2023-07-16 Revised:2023-10-19 Online:2024-03-15 Published:2024-04-03

林火强度对兴安落叶松林土壤氮循环功能基因的影响

舒洋^1,2, 陈金平⁵, 丁兆华⁵, 李航^3,4, 赵鹏武^1,2, 周梅^1,2, 贾文杰^1,2, 张轶超^1,2, 张国臣⁶, 魏江生^2,3,4

1. 内蒙古农业大学林学院, 内蒙古呼和浩特 010019;
2. 内蒙古赛罕乌拉森林生态系统国家定位观测研究站, 内蒙古赤峰 025150;
3. 内蒙古农业大学草原与资源环境学院, 内蒙古呼和浩特 010011;
4. 内蒙古自治区土壤质量与养分资源重点实验室, 内蒙古呼和浩特 010011;
5. 内蒙古根河森林工业有限公司, 内蒙古根河 022350;
6. 内蒙古赤峰市克什克腾旗桦木沟林场, 内蒙古赤峰 025350

通讯作者: 魏江生,E-mail:weijiangsheng1969@163.com
作者简介:舒洋(1988-)，男，汉族，内蒙古通辽人，讲师，主要从事森林生态学研究，E-mail:shuyang2018@imau.edu.cn
基金资助:
林火干扰对大兴安岭落叶松林土壤微生物氮循环影响机制研究(32001325)；2022年度自治区本级事业单位引进高层次人才科研支持经费(DC2300001275)；高层次/优秀博士人才引进科研启动项目(NDYB2022-18)；森林生态系统多样性的多维度格局及时空分异规律(2022YFF0801801)资助

Abstract

Abstract: To investigate the effect of forest fire intensity on soil nitrogen cycling,the abundance of soil nitrogen cycling functional genes and the physicochemical properties of soil were determined by quantitative PCR in different intensities of fire sample plots in the fire burn site of Larix Gmelinii forest in Da Xing'anling,Inner Mongolia. The results showed that the abundance of nitrogen-fixing nifH functional gene decreased with the increase in fire intensity,while the abundance of nitrifying amoA-AOA and amoA-AOB functional genes showed the opposite trend. Furthermore,the abundance of denitrifying nirS,nirK,and nosZ functional genes was significantly decreased after heavy fire,whereas these functional gene abundances were significantly increased after light fire. Fire also significantly affected the basic physical and chemical properties of soil. It had a close relationship between soil nitrogen cycle functional genes and soil physicochemical factors,in which organic carbon,quick-acting phosphorus,total nitrogen concent,and pH value were the main factors affecting the differential changes of soil nitrogen cycle functional genes after fire based by RDA analysis and Pearson correlation analysis. This study will provide data support for the mechanism of fire disturbance on soil nitrogen cycling and rapid post-fire vegetation regeneration.

Key words: Fire interference, Nitrogen cycling gene, Larix gmelinii, Soil nutrient cycling

摘要： 为了探究林火强度对土壤氮素循环的影响,本研究以内蒙古大兴安岭兴安落叶松林火烧迹地为研究对象,通过DNA提取及定量PCR分析测定了不同火烧强度样地内土壤氮循环功能基因丰度以及土壤基本的理化性质。结果表明:固氮nifH功能基因丰度随着火烧强度的增加而减少,而硝化amoA-AOA和amoA-AOB功能基因丰度呈相反变化趋势;反硝化nirS,nirK和nosZ功能基因丰度在重度火烧后显著降低,在轻度火烧后显著升高。火烧同样也会对土壤基本理化性质产生显著影响,RDA分析和Pearson相关性分析表明土壤氮循环功能基因与土壤理化因子之间存在密切的联系,有机碳、速效磷、全氮含量和pH值是影响火后土壤氮循环功能基因差异变化的主要因子。本研究为火干扰对土壤氮素循环的影响机制和火后植被快速更新提供数据支撑。

关键词: 火烧强度, 氮循环功能基因, 兴安落叶松, 土壤养分循环

CLC Number:

S762

SHU Yang, CHEN Jin-ping, DING Zhao-hua, LI Hang, ZHAO Peng-wu, ZHOU Mei, JIA Wen-jie, ZHANG Yi-chao, ZHANG Guo-chen, WEI Jiang-sheng. Effects of Forest Fire Intensity on Functional Genes of Soil Nitrogen Cycling in Larix Gmelinii[J]. Acta Agrestia Sinica, 2024, 32(3): 726-735.

舒洋, 陈金平, 丁兆华, 李航, 赵鹏武, 周梅, 贾文杰, 张轶超, 张国臣, 魏江生. 林火强度对兴安落叶松林土壤氮循环功能基因的影响[J]. 草地学报, 2024, 32(3): 726-735.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2024.03.008

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2024/V32/I3/726

References

[1] MANSOOR S,FAROOQ I,KACHROO M M,et al. Elevation in wildfire frequencies with respect to the climate change[J]. Journal of Environmental Management,2022,301:113769
[2] SENANDE-RIVERA M,INSUA-COSTA D,MIGUEZ-MACHO G. Spatial and temporal expansion of global wildland fire activity in response to climate change[J]. Nature Communications,2022,13(1):1208
[3] 杨莉琳,姚琦馥,梁琍,等. 土壤氮素内循环对生态覆被变化响应的研究进展[J]. 中国生态农业学报,2020,28(10):1543-1550
[4] HART S C,DELUCA T H,NEWMAN G S,et al. Post-fire vegetative dynamics as drivers of microbial community structure and function in forest soils[J]. Forest Ecology and Management,2005,220(1-3):166-184
[5] LOURENÇO L,FERNANDES S,BENTO-GONÇALVES A,et al. Causas de incêndios florestais em Portugal continental. Análise estatística da investigação efetuada no último quindénio (1996 a 2010)[J]. Cadernos de Geografia,2012(30-31):61-80
[6] 王礼霄,刘晋仙,柴宝峰. 华北亚高山土壤细菌群落及氮循环对退耕还草的响应[J]. 生态环境学报,2022,31(8):1537-1546
[7] 佘容,杨晓燕,肖文. 火干扰下的森林土壤微生物研究现状分析[J]. 四川林业科技,2021,42(3):94-101
[8] SU W Q,YU M,LIN J,et al. Fire decreases gross mineralization rate but does not alter gross nitrification rate in boreal forest soils[J]. Soil Biology and Biochemistry,2022,175:108838
[9] SUN H,SANTALAHTI M,PUMPANEN J,et al. Bacterial community structure and function shift across a northern boreal forest fire chronosequence[J]. Scientific Reports,2016,6(1):32411
[10] TAS。N,PRESTAT E,MCFARLAND J W,et al. Impact of fire on active layer and permafrost microbial communities and metagenomes in an upland Alaskan boreal forest[J]. The ISME Journal,2014,8(9):1904-1919
[11] 李顺,吴志伟,梁宇,等. 北方森林林火发生驱动因子及其变化趋势研究进展[J]. 世界林业研究,2017,30(2):41-45
[12] 朱光艳,胡同欣,李飞,等. 火后不同年限兴安落叶松林土壤氮的矿化速率及其影响因素[J]. 中南林业科技大学学报,2018,38(3):88-96
[13] 吴晞,赵雨森,辛颖. 大兴安岭火烧迹地植被恢复过程中土壤氮素特征[J]. 森林工程,2022,38(2):8-13,104
[14] 郝帅,王星,张秋良,等. 大兴安岭火烧迹地遥感提取研究[J]. 林业资源管理,2022(2):75-81
[15] 马秀枝,范雪松,舒常禄,等. 不同时间序列林火干扰对兴安落叶松林区土壤性质及温室气体通量的影响[J]. 生态环境学报,2016,25(6):939-946
[16] 孙红,田昕,闫敏,等. 内蒙古大兴安岭根河植被覆盖度动态变化及影响因素的分析[J]. 遥感技术与应用,2018,33(6):1159-1169
[17] 刘发林,陈小伟,曾素平. 不同火干扰强度对枫香次生林土壤理化性质的影响[J]. 水土保持学报,2019,33(5):132-138
[18] 鲁如坤.土壤农业化学分析方法[M].北京:中国农业科技出版社,2000:158-160
[19] BROOKES P C,KRAGT J F,POWLSON D S,et al. Chloroform fumigation and the release of soil nitrogen:the effects of fumigation time and temperature[J]. Soil Biology and Biochemistry,1985,17(6):831-835
[20] WANG Q,WANG C,YU W W,et al. Effects of nitrogen and phosphorus inputs on soil bacterial abundance,diversity,and community composition in Chinese fir plantations[J]. Frontiers in Microbiology,2018,9:1543
[21] THROBÄCK I N,ENWALL K,JARVIS Å,et al. Reassessing PCR primers targeting nirS,nirK and nosZ genes for community surveys of denitrifying bacteria with DGGE[J]. FEMS Microbiology Ecology,2004,49(3):401-417
[22] PALMER K,BIASI C,HORN M A. Contrasting denitrifier communities relate to contrasting N₂O emission patterns from acidic peat soils in arctic tundra[J]. The ISME Journal,2012,6(5):1058-1077
[23] RICH J J,HEICHEN R S,BOTTOMLEY P J,et al. Community composition and functioning of denitrifying bacteria from adjacent meadow and forest soils[J]. Applied and Environmental Microbiology,2003,69(10):5974-5982
[24] RÖSCH C,MERGEL A,BOTHE H. Biodiversity of denitrifying and dinitrogen-fixing bacteria in an acid forest soil[J]. Applied and Environmental Microbiology,2002,68(8):3818-3829
[25] ROTTHAUWE J H,WITZEL K P,LIESACK W. The ammonia monooxygenase structural gene amoA as a functional marker:molecular fine-scale analysis of natural ammonia-oxidizing populations[J]. Applied and Environmental Microbiology,1997,63(12):4704-4712
[26] FRANCIS C A,ROBERTS K J,BEMAN J M,et al. Ubiquity and diversity of ammonia-oxidizing archaea in water columns and sediments of the ocean[J]. Proceedings of the National Academy of Sciences,2005,102(41):14683-14688
[27] 陈小伟,刘发林,韩育明. 火干扰后枫香次生林不同土层土壤理化性质研究[J]. 自然灾害学报,2020,29(3):45-53
[28] PHILLIPS D H,FOSS J E,BUCKNER E R,et al. Response of surface horizons in an oak forest to prescribed burning[J]. Soil Science Society of America Journal,2000,64(2):754-760
[29] MAKOTO K,KOIKE T. Charcoal ecology:Its function as a hub for plant succession and soil nutrient cycling in boreal forests[J]. Ecological Research,2021,36(1):4-12
[30] WENINGER T,FILIPOVIC' V,MESIC' M,et al. Estimating the extent of fire induced soil water repellency in Mediterranean environment[J]. Geoderma,2019,338:187-196
[31] AROCENA J M,OPIO C. Prescribed fire-induced changes in properties of sub-boreal forest soils[J]. Geoderma,2003,113(1-2):1-16
[32] VALKÓ O,DEÁK B,MAGURA T,et al. Supporting biodiversity by prescribed burning in grasslands-A multi-taxa approach[J]. Science of The Total Environment,2016,572:1377-1384
[33] 范雪松,马秀枝,任乐,等. 林火干扰对寒温带兴安落叶松土壤微生物数量的影响[J]. 林业资源管理,2014(6):115-120
[34] 陆刚,黄海霞,周晓雷,等. 迭部林区云冷杉林火烧迹地土壤有机碳及酶活性变化特征[J]. 草地学报,2022,30(4):943-949
[35] 周文昌,牟长城,刘夏,等. 火干扰对小兴安岭白桦沼泽和落叶松-苔草沼泽凋落物和土壤碳储量的影响[J]. 生态学报,2012,32(20):6387-6395
[36] 谷会岩,金靖博,陈祥伟,等. 不同火烧强度林火对大兴安岭北坡兴安落叶松林土壤化学性质的长期影响[J]. 自然资源学报,2010,25(7):1114-1121
[37] 薛伟,沙聪,张华超,等. 长白落叶松人工林采伐迹地土壤火灾温度场试验研究[J]. 应用生态学报,2019,30(3):785-792
[38] ALBERT-BELDA E,HINOJOSA M B,LAUDICINA V A,et al. Previous fire occurrence,but not fire recurrence,modulates the effect of charcoal and ash on soil C and N dynamics in Pinus pinaster Aiton forests[J]. Science of The Total Environment,2022,802:149924
[39] 张琳,王益,舒梦,等. 火烧对黄土高原草地土壤氮素净矿化速率和净硝化速率的影响[J]. 南京农业大学学报,2017,40(6):1051-1057
[40] CONDRON L,STARK C,O'CALLAGHAN M,et al. The role of microbial communities in the formation and decomposition of soil organic matter[J]. Soil Microbiology and Sustainable Crop Production,2010:81-118
[41] FERNÁNDEZ-GARCÍA V,MARCOS E,FERNÁNDEZ-GUISURAGA J M,et al. Impact of burn severity on soil properties in a Pinus pinaster ecosystem immediately after fire[J]. International Journal of Wildland Fire,2019,28(5):354-364
[42] MOYA D,GONZÁLEZ-DE VEGA S,LOZANO E,et al. The burn severity and plant recovery relationship affect the biological and chemical soil properties of Pinus halepensis Mill. stands in the short and mid-terms after wildfire[J]. Journal of Environmental Management,2019,235:250-256
[43] 冉益倩,周俊,张茜彧,等. 火烧强度对西南亚热带林地土壤理化性质的影响[J]. 草地学报,2023,31(9):2796-2804
[44] 么中元,张乃莉,崔喜艳. 火烧和施氮对内蒙古半干旱草原土壤微生物群落碳源利用潜力的影响[J]. 草地学报,2014,22(4):713-721
[45] 孔健健,杨健. 林火对大兴安岭落叶松林土壤性质的短期与长期影响[J]. 生态学杂志,2014,33(6):1445-1450
[46] 徐欢,王芳芳,李婷婷,等.冻融交替对土壤氮素循环关键过程的影响与机制研究进展[J].生态学报,2020,40(10):3168-3182
[47] 李伟克,刘晓东,牛树奎,等. 火烧对河北平泉油松林土壤微生物量的影响[J]. 北京林业大学学报,2017,39(10):70-77
[48] 张亨宇,沈建辛,张洪波,等. 林火对大兴安岭森林土壤微生物的影响[J]. 东北林业大学学报,2019,47(7):99-102
[49] 宋良翠,马维伟,李广,等. 温度对尕海退化湿地土壤硝化潜力的影响[J]. 草地学报,2021,29(9):2058-2066
[50] DELUCA T H,MACKENZIE M D,GUNDALE M J,et al. Wildfire-produced charcoal directly influences nitrogen cycling in ponderosa pine forests[J]. Soil Science Society of America Journal,2006,70(2):448-453
[51] 刘鑫军,魏洪杰. 氮添加对樟子松人工林氮转化及相关功能基因丰度的影响[J]. 广西植物,2022,42(7):1077-1087
[52] YERMAKOV Z,ROTHSTEIN D E. Changes in soil carbon and nitrogen cycling along a 72-year wildfire chronosequence in Michigan jack pine forests[J]. Oecologia,2006,149:690-700
[53] ROMEO F,MARZILIANO P A,TURRIÓN M B,et al. Short-term effects of different fire severities on soil properties and Pinus halepensis regeneration[J]. Journal of Forestry Research,2020,31:1271-1282
[54] GARCIA-CARMONA M,MARIN C,GARCIA-ORENES F,et al. Contrasting organic amendments induce different short-term responses in soil abiotic and biotic properties in a fire-affected native Mediterranean forest in Chile[J]. Journal of Soil Science and Plant Nutrition,2021,21(3):2105-2114
[55] 曹林桦,刘彩霞,刘茗,等. 集约经营对毛竹林土壤反硝化细菌丰度的影响[J]. 土壤学报,2020,57(3):710-720
[56] 娄焕杰,邓焕广,王东启,等. 上海城市河岸带土壤反硝化作用研究[J]. 环境科学学报,2013,33(4):1118-1126
[57] KÖSTER K,AALTONEN H,BERNINGER F,et al. Impacts of wildfire on soil microbiome in Boreal environments[J]. Current Opinion in Environmental Science & Health,2021,22:100258
[58] MIKITA-BARBATO R A,KELLY J J,TATE III R L. Wildfire effects on the properties and microbial community structure of organic horizon soils in the New Jersey Pinelands[J]. Soil Biology and Biochemistry,2015,86:67-76
[59] 丁凯,张毓婷,张俊红,等. 不同密度杉木林对林下植被和土壤微生物群落结构的影响[J]. 植物生态学报,2021,45(1):62-73
[60] 樊梓豪,范丹丹,王梦梦,等. 生物细胞中颗粒状多聚磷酸盐细胞器的结构与功能[J]. 微生物学报,2022,62(12):4713-4730
[61] 屈明华,俞元春,李生,等. 丛枝菌根真菌对矿质养分活化作用研究进展[J]. 浙江农林大学学报,2019,36(2):394-405
[62] ROMEO F,MARZILIANO P A,TURRIÓN M B,et al. Short-term effects of different fire severities on soil properties and Pinus halepensis regeneration[J]. Journal of Forestry Research,2020,31:1271-1282
[63] WAKELIN S A,MACDONALD L M,ROGERS S L,et al. Habitat selective factors influencing the structural composition and functional capacity of microbial communities in agricultural soils[J]. Soil Biology and Biochemistry,2008,40(3):803-813(责任编辑闵芝智)第32卷第3期 Vol.32 No. 3草地学报 ACTAAGRESTIASINICA 2024年 3月

Effects of Forest Fire Intensity on Functional Genes of Soil Nitrogen Cycling in Larix Gmelinii

林火强度对兴安落叶松林土壤氮循环功能基因的影响

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 1

Recommended Articles

Metrics

Comments