Abstract: This experiment treated two types of C. rigescens,'Lv Ping No.1' and 'Lv Ping No.2'with 0,100,200,and 300 mmol·L^-1 NaCl for 21 days. The chlorophyll content,relative electrolytic leakage,relative water content,H₂O₂ content,malondialdehyde content,K⁺/Na⁺ ratio,net photosynthetic rate,stomatal conductance,intercellular carbon dioxide concentration,transpiration rate,superoxide dismutase,and peroxidase activity indicators were measured. Membership function analysis and comparison were conducted on each measured value. The salt tolerance characteristics of two species of C. rigescens under long-term salt stress were explored. The results showed that the content of MDA and H₂O₂ content in 'Lv Ping No.1' were significantly increased after treated with a salt concentration of 300 mmol·L^-1,comparing to the control group. The activity changes of superoxide dismutase and peroxidase in the two materials are inconsistent. Subsequently,through correlation analysis,principal component analysis,and membership function analysis,three principal components were extracted as comprehensive indicators for evaluating the salt tolerance of C. rigescens. After calculation,it was determined that 'Lv Ping No.2'had the stronger salt tolerance. The results of this study enhance the utilization of C. rigescens plants in saline alkali land,which also provide useful information for subsequent research on its salt tolerance mechanisms.

Key words: Carex rigescens, Salt stress, Antioxidant enzyme activity, Membership function, Comprehensive evaluation

摘要： 本试验以两个白颖苔草(Carex rigescens)品种'绿坪1号'和'绿坪2号'为材料,使用0,100,200和300 mmol·L^-1NaCl处理21 d,对处理后白颖苔草叶片进行了叶绿素含量、相对电导率、相对含水量、过氧化氢含量、丙二醛含量、钾钠比、净光合速率、气孔导度、胞间二氧化碳浓度、蒸腾速率、超氧化物歧化酶和过氧化物酶活性指标的测定,并对各测定值进行隶属函数分析比较,探究两种白颖苔草在长期盐胁迫下的耐盐特性。结果表明:'绿坪1号'中丙二醛含量和过氧化氢含量在盐浓度300 mmol·L^-1处理后比对照相比有更大幅度的上升;两材料中超氧化物歧化酶和过氧化物酶活性变化不一致。随后经过相关性分析、主成分分析和隶属函数分析,提取3个主成分作为白颖苔草耐盐性评价的综合指标,经过计算得出耐盐性较强的是'绿坪2号'。研究结果对白颖苔草在盐碱地利用及后续耐盐机理研究具有参考意义。

关键词: 白颖苔草, 盐胁迫, 抗氧化酶活性, 隶属函数, 综合评价