Effects of Waterlogging and Biochar Addition on the Growth and Antioxidant Enzyme Activity of Alfalfa Seedlings

doi:10.11733/j.issn.1007-0435.2024.04.011

Acta Agrestia Sinica ›› 2024, Vol. 32 ›› Issue (4): 1087-1093.DOI: 10.11733/j.issn.1007-0435.2024.04.011

Effects of Waterlogging and Biochar Addition on the Growth and Antioxidant Enzyme Activity of Alfalfa Seedlings

ZHOU Zhi-dong¹, ZHANG Yu¹, LI Xiang-ru¹, YANG Shi-jian¹, YANG Jing¹, TAN Jing¹, LIU Ying-cheng¹, CHEN Yuan-qi^2,3

1. School of Life and Health Sciences, Hunan Province Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization, Hunan University of Science and Technology, Xiangtan, Hunan Province 411201, China;
2. School of Earth Science and Spatial Information Engineering, Hunan Province Key Laboratory of Coal Resources Clean-utilization and Mine Environment Protection, Hunan University of Science and Technology, Xiangtan, Hunan Province 411201, China;
3. Institute of Geographical Environment and Carbon Peak and Neutrality, Hunan University of Science and Technology, Xiangtan, Hunan Province 411201, China

Received:2023-09-26 Revised:2023-11-26 Published:2024-05-07

渍水和生物炭对紫花苜蓿幼苗生长和抗氧化酶活性的影响

周志东¹, 张煜¹, 李湘如¹, 杨世鉴¹, 杨菁¹, 谭婧¹, 刘莹成¹, 陈远其^2,3

1. 湖南科技大学生命科学与健康学院经济作物遗传改良与综合利用湖南省重点实验室, 湖南湘潭 411201;
2. 湖南科技大学地球科学与空间信息工程学院煤炭资源清洁利用与矿山环境保护湖南省重点实验室, 湖南湘潭 411201;
3. 湖南科技大学地理环境与双碳研究所, 湖南湘潭 411201

通讯作者: 陈远其,E-mail:chenyq2016@163.com
作者简介:周志东(2001-)，男，汉族，湖南郴州人，硕士研究生，主要从事植物生态学研究，E-mail:2325553601@qq.com
基金资助:
湖南省自然科学基金(2018 JJ3148)；湖南省教育厅科学研究项目(23A0385,21C0359)；湖南省经济作物遗传改良与综合利用重点实验室科学基金开放项目(E22117) 资助

Abstract

Abstract: The adapting mechanism of legume plants as pioneer species in degraded ecosystems is still unclear in the purple soil area. Therefore,in this study,the Medicago sativa L. 'Sanditi’ was selected and planted in the purple soil collected from a subtropical hilly slope. This pot experiment with the treatments of waterlogging and biochar addition (0.5%,w/w) was conducted in a greenhouse. The plant height,root length,biomass (above,below,and total),leaf catalase (CAT) and peroxidase (POD) activities of M. sativa were investigated. The results showed that waterlogging treatment only increased its stem length and total length. Biochar addition significantly increased plant height and biomass,but decreased the ratio of root-to-shoot length,the ratio of root biomass to aboveground biomass,and CAT and POD activities. These effects of biochar addition were also observed under the condition of waterlogging. Moreover,there was a longer term effect of biochar addition on CAT activity than on POD activity. These findings indicated that M. sativa could adapt to a certain waterlogged environment,and biochar could be applied to the waterlogged purple soil as an ameliorant. Biochar addition could promote the growth and biomass accumulation of M. sativa. Together,this study provides a case reference and data support for planting and management of M. sativa in the hot and humid southern region of China.

Key words: Alfalfa, Water stress, Biochar, Antioxidant enzymes, Biomass

摘要： 豆科植物作为退化生态系统植被恢复的先锋物种在紫色土区的适应机理尚不清楚。本研究以紫花苜蓿'三得利’(Medicago sativa L.'Sanditi’)为研究对象，紫色土为基质，通过温室盆栽实验，进行渍水和生物炭添加(0.5%，质量比)处理，研究植物株高、根长、生物量和叶片过氧化氢酶(Catalase,CAT)与过氧化物酶(Peroxidase,POD)活性等。结果表明：渍水处理仅增加植物茎长和全长；生物炭添加处理增加植物株高、地上部分生物量和总生物量，降低植物根茎长度比、根冠比和CAT与POD活性，且上述效应在渍水条件下也同样存在；生物炭添加对CAT活性影响的时间长于POD。综上，紫花苜蓿能适应一定的渍水环境，生物炭可作为紫色土渍水环境的改良剂，并能够促进紫花苜蓿生长与生物量的累积。本研究为紫花苜蓿在南方湿热地区种植与管理提供了案例参考和数据支撑。

关键词: 紫花苜蓿, 水分胁迫, 生物炭, 抗氧化酶, 生物量

CLC Number:

S541

ZHOU Zhi-dong, ZHANG Yu, LI Xiang-ru, YANG Shi-jian, YANG Jing, TAN Jing, LIU Ying-cheng, CHEN Yuan-qi. Effects of Waterlogging and Biochar Addition on the Growth and Antioxidant Enzyme Activity of Alfalfa Seedlings[J]. Acta Agrestia Sinica, 2024, 32(4): 1087-1093.

周志东, 张煜, 李湘如, 杨世鉴, 杨菁, 谭婧, 刘莹成, 陈远其. 渍水和生物炭对紫花苜蓿幼苗生长和抗氧化酶活性的影响[J]. 草地学报, 2024, 32(4): 1087-1093.

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Effects of Waterlogging and Biochar Addition on the Growth and Antioxidant Enzyme Activity of Alfalfa Seedlings

渍水和生物炭对紫花苜蓿幼苗生长和抗氧化酶活性的影响

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