Genetic Diversity Analysis of Zoysia willd. Accessions by EST-SSR Markers

doi:10.11733/j.issn.1007-0435.2024.08.006

Acta Agrestia Sinica ›› 2024, Vol. 32 ›› Issue (8): 2386-2393.DOI: 10.11733/j.issn.1007-0435.2024.08.006

Genetic Diversity Analysis of Zoysia willd. Accessions by EST-SSR Markers

HUANG Chun-qiong¹, HE Xiao^1,2, LUO Li-juan², DONG Rong-shu¹, LIU Guo-dao¹

1. Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rural Affairs, Haikouu, Hainan Province 571101, China;
2. College of Tropical Crops Hainan University, Haikou, Hainan Province 570228, China

Received:2024-01-10 Revised:2024-03-26 Published:2024-09-07

结缕草属种质资源EST-SSR遗传多样性分析

黄春琼¹, 何潇^1,2, 罗丽娟², 董荣书¹, 刘国道¹

1. 中国热带农业科学院热带作物品种资源研究所/农业部华南作物基因资源与种质创制重点实验室, 海南海口 571101;
2. 海南大学热带作物学院, 海南海口 570228

通讯作者: 罗丽娟,E-mail:luoljd@126.com
作者简介:黄春琼(1982-),女,汉族,云南宣威人,博士,研究员,主要从事热带草种质资源与育种研究,E-mail:huangchunqiong44@163.com
基金资助:
海南省自然科学基金(322RC770;323RC536);国家现代农业产业技术体系(CARS-34)资助

Abstract

Abstract: In the present study,43 Zoysia willd. accessions from different sources were used to detect the genetic diversity using Expressed sequence tag-simple sequence repeat(EST-SSR) primers. The results showed that 30 pairs of primer from the original 125 pairs of primer were selected based on the polymorphic,clear and reproducible bands amplified by them. A total of 124 bands were amplified by 30 primer pairs from 43 accessions with 4 bands per primer pairs,and 108 (88.60%) out of them were polymorphic. The genetic similarity coeffcients (GSC) among the 43 accessions ranged from 0.530 to 0.864 with an average of 0.711. A total of 104 observing alleles were amplified by 30 pairs of primer from 43 accessions with 3 observed alleles per pairs of primer. The range of effective number of alleles was 0 to 5 with an average of 3. The range of shannon information index was 0～1.593 with an average of 0.940. The Nei’s genetic diversity index was 0～0.793 with an average of 0.541. Cluster analysis conducted by the unweighted pair-group method with arithmetic averages (UPGMA) separated the 43 accessions into 4 groups when the genetics similarity coefficient was 0.800. The clustering results were somewhat correlated with the geographical location of the accessions,but not closely related. The results indicated that the genetic diversity of Zoysia willd. accessions was rich,which laid a foundation for the identification and evaluation of Zoysia willd. accessions and molecular marker-assisted breeding.

Key words: Zoysia willd, EST-SSR, Molecular makers, Geneic diversity, Population structure

摘要： 本研究以43份不同来源的结缕草属种质为材料，利用表达序列标签-简单重复序列(Expressed Sequence Tag-Simple sequence repeat，EST-SSR)引物对其进行遗传多样性分析。结果表明：从125对EST-SSR引物中筛选出30对能稳定扩增、条带清晰的引物；共检测到124个标记，平均每对引物检测到4个，多态性标记有108个，多态性位点百分率为88.60%；种质间遗传相似性系数为0.530～0.864，平均为0.711；共检测到104个观察等位基因，平均每对引物检测到3个；有效等位基因数范围为0～5，平均为3；Shannon信息指数变化范围为0～1.593，平均为0.940。Nei’s基因多样性指数范围为0～0.793，平均为0.541；在遗传相似性系数为0.800时，利用非加权组平均法(Unweighted pair-group method with arithmetic means,UPGMA)法将43份种质划分为4个类群，聚类结果与种质地理位置有一定的关联，但联系不紧密。本研究结果表明43份种质的遗传多样性处于上等水平，EST-SSR标记能有效地评价结缕草属种质的遗传差异，为结缕草属种质资源的鉴定评价及分子标记辅助育种提供参考依据。

关键词: 结缕草属, EST-SSR, 分子标记, 遗传多样性, 群体结构

CLC Number:

S543

HUANG Chun-qiong, HE Xiao, LUO Li-juan, DONG Rong-shu, LIU Guo-dao. Genetic Diversity Analysis of Zoysia willd. Accessions by EST-SSR Markers[J]. Acta Agrestia Sinica, 2024, 32(8): 2386-2393.

黄春琼, 何潇, 罗丽娟, 董荣书, 刘国道. 结缕草属种质资源EST-SSR遗传多样性分析[J]. 草地学报, 2024, 32(8): 2386-2393.

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Genetic Diversity Analysis of Zoysia willd. Accessions by EST-SSR Markers

结缕草属种质资源EST-SSR遗传多样性分析

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