Acta Agrestia Sinica

Acta Agrestia Sinica ›› 2025, Vol. 33 ›› Issue (10): 3215-3224.DOI: 10.11733/j.issn.1007-0435.2025.10.009

Previous Articles    

Bacterial Community Structure and Diversity in Rhizosphere Soil in Caragana tibetica Desert Grassland

HAN Yan-long1,2, YANG Wen-yuan1, HAN Min1, GAO Yong1,2, YUAN Xiao-man1, YAN Ru3   

  1. 1. College of Desert Control Science and Engineering, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, China;
    2. Inner Mongolia Hangjin Desert Ecosystem Positioning and Observatory Station, Hohhot, Inner Mongolia 010018, China;
    3. Damao Banner Forestry and Grassland Workstation, Baotou, Inner Mongolia 014000, China
  • Received:2024-10-04 Revised:2024-11-27 Published:2025-10-17

荒漠草原毛刺锦鸡儿灌丛根际土壤细菌群落结构及多样性

韩彦隆1,2, 杨文源1, 韩敏1, 高永1,2, 袁晓满1, 燕茹3   

  1. 1. 内蒙古农业大学, 沙漠治理学院, 内蒙古 呼和浩特 010018;
    2. 内蒙古杭锦荒漠生态系统定位观测研究站, 内蒙古 呼和浩特 010018;
    3. 达茂旗林业和草原工作站, 内蒙古 包头 014000
  • 通讯作者: 高永,E-mail:13948815709@163.com
  • 作者简介:高永,E-mail:13948815709@163.com
  • 基金资助:
    国家自然科学基金项目（4226070139）资助

PDF

Abstract: In this study， the soil bacterial community composition and diversity characteristics across different-sized Caragana tibetica shrub mounds [large shrub（LS）， medium shrub（MS）， small shrub（SS）] and non-shrub Control Check （CK） in a desert steppe of Damao Banner were investigated. Combining 16SrRNA high-throughput sequencing with soil physicochemical analysis， the soil bacterial community structure and diversity of different specifications （large， medium and small） shrublands and the control plots （CK） without shrubland growth， as well as their relationship with the physical and chemical properties of the soil were explored. The results showed that significant differences （P<0.05） in soild-protease， moisture content， and TN were observed between shrub rhizosphere soils and CK. Actinobacteria， Chloroflexota， and Proteobacteria dominated across all samples， with Nakamurella and Baekduia being predominant genera. OTU （Operational taxonomic unit） numbers were ranked as SS>LS>CK>MS， while significant inter-group variations （P<0.05） emerged in alpha diversity indices （Observed-species， Chao1， and ACE）. Beta diversity analysis showed a stress value of 0.0679 confirming cluster reliability. Soil pH， TP， and AN significant correlations （P<0.05） with Chloroflexota abundance， and RDA （Redundancy analysis） identified soild-protease， TN， and soild-urease as key drivers of bacterial community composition. These findings collectively reveal distinct soil bacterial community structures between shrub mounds and CK， highlighting the critical the regulatory role of soil environmental factors in shaping microbial communities.

Key words: Desert steppe, Caragana tibetica, Rhizosphere soil, Bacterial community composition, Bacterial diversity

摘要： 本研究以荒漠草原达茂旗毛刺锦鸡儿（Caragana tibetica）灌丛沙堆为研究对象，通过16S rRNA高通量测序和土壤理化性质分析，探究不同规格（大、中、小）灌丛及无灌丛生长的对照地（CK）土壤细菌群落结构和多样性以及与土壤理化性质的关系。结果表明：不同规格灌丛根际土和对照地（CK）土壤样品中，蛋白酶活性、含水量和全氮含量存在显著差异（P<0.05）；根际土与对照地（CK）土壤优势菌门为放线菌门、绿弯菌门和变形菌门，优势菌属为中村氏菌属、长白山菌属等；操作分类单元（Operational taxonomic unit，OTU）数排序为小灌丛>大灌丛>对照地（CK）>中灌丛，其中Alpha多样性的物种数、Chao1和ACE指数存在显著差异（P<0.05），Beta多样性分析Stress值为0.0679，结果可靠；土壤pH值、全磷和碱解氮含量与绿弯菌门丰度存在显著相关关系（P<0.05），RDA分析表明蛋白酶活性、全氮含量和脲酶活性是驱动细菌群落组成的关键因子。综上所述，不同规格灌丛根际土与对照地（CK）土壤细菌群落存在一定差异，且土壤环境因子对微生物群落产生影响。

关键词: 荒漠草原, 毛刺锦鸡儿, 根际土壤, 细菌群落结构, 细菌多样性

CLC Number: