Effects of Alfalfa on Soil Fungal Community Formation in the Eastern Qinghai-Tibet Plateau

doi:10.11733/j.issn.1007-0435.2025.10.010

Acta Agrestia Sinica ›› 2025, Vol. 33 ›› Issue (10): 3225-3236.DOI: 10.11733/j.issn.1007-0435.2025.10.010

Effects of Alfalfa on Soil Fungal Community Formation in the Eastern Qinghai-Tibet Plateau

WEN Yan-bin^1,2, WANG Xin^1,2, WANG Na^1,2, SONG Hui-yu^1,2, DU Yan-gong¹

1. Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai Province 810008, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China

Received:2024-10-16 Revised:2024-12-30 Published:2025-10-17

紫花苜蓿对青藏高原东部土壤真菌群落的影响

文焱彬^1,2, 王新^1,2, 王娜^1,2, 宋慧宇^1,2, 杜岩功¹

1. 中国科学院西北高原生物研究所, 青海西宁 810008;
2. 中国科学院大学, 北京 100049

通讯作者: 杜岩功,E-mail:ygdu@nwipb.cas.cn
作者简介:杜岩功,E-mail:ygdu@nwipb.cas.cn
基金资助:
国家重点研发计划青藏高原典型自然保护地生态系统保护恢复及多功能提升技术与示范（2023YFF1304302）；国家自然科学基金（U21A20186）资助

Abstract

Abstract: This study focused on Medicago sativa L. ecosystems in Maqin County， Guinan County， and Guide County of Qinghai Province. Soil samples were collected from both alfalfa cultivation plots and adjacent control plots. Soil nutrient contents were determined using standard analytical methods， and soil fungal communities were characterized using second-generation amplicon sequencing.The results indicated that alfalfa cultivation in Maqin and Guinan increased soil nutrient levels and reduced fungal community diversity and evenness， although these changes were not statistically significant. The fungal communities were dominated by Ascomycota， ranging from 36.58% to 69.24%， and Basidiomycota， ranging from 8.02% to 33.41%. There were substantial differences in beta diversity among regions， and mean annual temperature was significantly correlated with the composition of soil fungal communities at the 0.05 level. Fungal interactions were primarily synergistic， and the fungal co-occurrence network maintained structural stability by enhancing robustness through the loss of keystone taxa. The assembly of fungal communities was mainly governed by stochastic processes， with the neutral model explaining most of the variation in microbial community structure.This study provides scientific evidence supporting the ecological rationality of incorporating Medicago sativa into alpine grassland ecosystems.

Key words: Alfalfa, Soil nutrients, Fungal community, Co-occurrence network, Community assembly process

摘要： 本文以青海省玛沁县、贵南县和贵德县紫花苜蓿（Medicago sativa L.）生态系统为研究对象，采集紫花苜蓿田间和空白地块土壤通过常规方法测定养分含量，通过二代扩增子测序测定土壤真菌。结果表明：紫花苜蓿在玛沁和贵南的种植增加了土壤养分含量，降低了真菌群落的多样性和均匀度，但未达到显著差异水平；真菌群落以子囊菌门（36.58%~69.24%）、担子菌门（8.02%~33.41%）为主，区域间β多样性差异较大，且年均温同土壤真菌群落有显著的相关关系（P<0.05）；真菌群落间以协同为主，真菌网络通过丢失关键节点提高稳健性，维持网络结构稳定；真菌群落由随机性过程主导，中性模型解释了大多数的微生物群落变化。本研究为紫花苜蓿纳入高寒草地生态系统的合理性提供了科学依据。

关键词: 紫花苜蓿, 土壤养分, 真菌群落, 共现网络, 群落组装过程

CLC Number:

S541.9

WEN Yan-bin, WANG Xin, WANG Na, SONG Hui-yu, DU Yan-gong. Effects of Alfalfa on Soil Fungal Community Formation in the Eastern Qinghai-Tibet Plateau[J]. Acta Agrestia Sinica, 2025, 33(10): 3225-3236.

文焱彬, 王新, 王娜, 宋慧宇, 杜岩功. 紫花苜蓿对青藏高原东部土壤真菌群落的影响[J]. 草地学报, 2025, 33(10): 3225-3236.

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Effects of Alfalfa on Soil Fungal Community Formation in the Eastern Qinghai-Tibet Plateau

紫花苜蓿对青藏高原东部土壤真菌群落的影响

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