Acta Agrestia Sinica ›› 2025, Vol. 33 ›› Issue (10): 3341-3352.DOI: 10.11733/j.issn.1007-0435.2025.10.022

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Evaluation on Salt Tolerance of 18 Alfalfa Cultivars During Germination

WANG Yan, SHI Shang-li, YU Hai-tao, HAN Yi-lin   

  1. College of Prataculture, Gansu Agricultural University/Key Laboratory of Prataculture Ecosystem of Ministry of Education/China-US Research Center for Sustainable Development of Grassland Animal Husbandry, Lanzhou, Gansu Province 730070, China
  • Received:2024-12-10 Revised:2025-01-15 Online:2025-10-15 Published:2025-10-17

18个紫花苜蓿品种萌发期耐盐性评价

汪艳, 师尚礼, 于海涛, 韩宜霖   

  1. 甘肃农业大学草业学院/草业生态系统教育部重点实验室/中-美草地畜牧业可持续发展研究中心, 甘肃 兰州 730070
  • 通讯作者: 师尚礼,E-mail:shishl@gasu.edu.cn
  • 作者简介:师尚礼,E-mail:shishl@gasu.edu.cn
  • 基金资助:
    苜蓿燕麦高产种质创新和品种选育子课题(23ZDKA013);国家现代农业产业体系(CARS-34)资助

Abstract: To identify the salt tolerance of different varieties of alfalfa (Medicago sativa L.) and screen varieties with different salt tolerance, 18 alfalfa varieties at home and abroad were used as test materials. Different concentrations (0.4%, 0.5%, 0.6%, 0.7% and 0.8%) NaCl salt solution were used to give a stress treatment to the seeds at germination stage. Indicators such as germination rate (GR), germination potential (GP), relative germination percentage (RGP), relative germination rate (RGR), vigor index (VI), germination index (GI), plumule length (PL) and radicle length (RL) were measured. The germination rate (GR), relative germination percentage (RGP), germination index (GI), vigor index (VI), plumule length (PL) and Radicle length (RL) were analyzed under the NaCl concentration gradient treatments, and the main character indexes of salt damage were analyzed. With the increase of NaCl stress concentration, germination rate, relative germination potential, germination index, vitality index, germ length and radicle all showed a decreasing trend. The results showed that the first principal component had the largest eigenvalue, which was 2.907, and its contribution rate was as high as 36.335%. The indexes with large eigenvectors were GR, GP, GI, VI and RL. By membership function method, weight coefficient and comprehensive evaluation D value analysis, it can be seen that‘GongnongNo.1’ and ‘Gannong No.3’ alfalfa have strong tolerance to salt damage, and can tolerate 0.6% NaCl stress, and 0.7% NaCl mass fraction can inhibit the germination of tested alfalfa seeds.

Key words: Alfalfa, Salt stress, Germination characteristics, Salt tolerance evaluation

摘要: 为鉴定不同紫花苜蓿(Medicago sativa L.)品种的耐盐性和筛选不同耐盐能力的品种,以国内外18个紫花苜蓿品种为试验材料,设置不同浓度(0.4%,0.5%,0.6%,0.7%,0.8%)的NaCl盐溶液对萌发期的种子进行胁迫处理,测定其发芽率(Germination rate, GR)、发芽势(Germination potential,GP)、相对发芽势(Relative germination percentage,RGP)、相对发芽率(Relative germination rate, RGR)、活力指数(Vigor index,VI)、发芽指数(Germination index,GI)、胚芽长(Plumule length, PL)和胚根长(Radicle length, RL)等指标,分析NaCl浓度梯度处理下的发芽情况,以及盐害的主要性状指标;随着NaCl胁迫浓度的升高,发芽率、相对发芽势、发芽指数、活力指数、胚芽长、胚根长均呈现降低趋势,结果表明:特征值最大的是第1主成分,为2.907,其贡献率高达36.335%,具有较大的特征向量的指标为GR,GP,GI,VI,RL。通过隶属函数法、权重系数和综合评价D值分析可知,‘公农1号’和‘甘农3号’耐受盐害能力较强,可耐0.6%NaCl胁迫,0.7% NaCl质量分数对供试苜蓿种子的萌发具有抑制作用。

关键词: 紫花苜蓿, 盐胁迫, 萌发特性, 耐盐性评价

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