Isolation and Identification of Pathogens Causing Alfalfa Root Rot in Hohhot and Analysis of Strain Pathogenicity

doi:10.11733/j.issn.1007-0435.2025.10.031

Acta Agrestia Sinica ›› 2025, Vol. 33 ›› Issue (10): 3438-3447.DOI: 10.11733/j.issn.1007-0435.2025.10.031

Isolation and Identification of Pathogens Causing Alfalfa Root Rot in Hohhot and Analysis of Strain Pathogenicity

JIA Lei^1,2, HE Jie-pu^1,2, WU Chong-bin^1,2, GUO Yu-han^1,2, LIU Xin^1,2, SHI Yun^1,2, BAI Jia-he^1,2, XU Rui^1,2, SHEN Hong-yan³, ZHAO Ya-ru³, DING Wang⁴, CONG Li-li^1,2,5

1. College of Grassland Science, Qingdao Agricultural University, Qingdao, Shandong Province 266109, China;
2. Key Laboratory of Grassland Resources and Ecology, National Forestry and Grassland Administration, Yellow River Delta, Qingdao, Shandong Province 266109, China;
3. Inner Mongolia Zhengshi Grassland Industry Co., Ltd., Hohhot, Inner Mongolia 010000, China;
4. Golas (Beijing) Technology Development Co., Ltd., Beijing 101399, China;
5. The Biotechnological Breeding Innovation Center of Inner Mongolia Autonomous Region, Ulanhot, Inner Mongolia 137400, China

Received:2024-12-26 Revised:2025-02-03 Published:2025-10-17

呼和浩特苜蓿根腐病病原菌分离鉴定及菌株致病性分析

贾磊^1,2, 何介普^1,2, 伍崇斌^1,2, 郭雨寒^1,2, 刘信^1,2, 石昀^1,2, 白家赫^1,2, 徐瑞^1,2, 沈宏燕³, 赵雅茹³, 丁旺⁴, 丛丽丽^1,2,5

1. 青岛农业大学草业学院, 山东青岛 266109;
2. 黄河三角洲草地资源与生态国家林业和草原局重点实验室, 山东青岛 266109;
3. 内蒙古正时草业有限责任公司, 内蒙古呼和浩特 010000;
4. 歌拉斯(北京)发展有限公司, 北京 101399;
5. 内蒙古自治区生物育种创新中心, 内蒙古乌兰浩特 137400

通讯作者: 丁旺,E-mail:dingwang1017@126.com;丛丽丽,E-mail:dcongli1985610@126.com
作者简介:丁旺,E-mail:dingwang1017@126.com;丛丽丽,E-mail:dcongli1985610@126.com
基金资助:
草地资源教育部重点实验室开放课题（RC1900004958）；内蒙古自治区生物育种技术创新中心2024年科技支撑课题（2024NSZC03-003）：苜蓿属抗根腐病种质资源鉴定、侵染机制研究与新种质创制资助

Abstract

Abstract: Root rot is a major disease affecting alfalfa production in China， known as the “cancer” of alfalfa （Medicago sativa L.） due to its wide prevalence and difficult prevention and control. To identify the pathogens causing alfalfa root rot in the Hohhot region of Inner Mongolia， diseased tissues were collected through field sampling and isolated using conventional tissue isolation methods. Pathogens were purified and identified based on morphological characteristics combined with ITS and EF1-α gene sequence analysis， with pathogenicity confirmed through inoculation tests. The results revealed five Fusarium species： F. solani， F. oxysporum， F. redolens， F. nygamai， and F. falciforme. Pathogenicity analysis demonstrated that all five species could induce alfalfa root rot. Among them F. solani and F. oxysporum exhibited stronger pathogenicity. These two species were identified as the primary pathogens responsible for the severe occurrence of alfalfa root rot in the investigated areas of Hohhot.

Key words: Alfalfa, Root rot disease, Fusarium, Molecular identification, Pathogenicity

摘要： 根腐病是影响我国苜蓿（Medicago sativa L.）生产的主要病害，因其发病范围广、防控难度大，被称为苜蓿的“癌症”。为探明内蒙古呼和浩特地区苜蓿根腐病的病原菌，经田间采样收集病组织，采用常规组织分离法分离纯化病原菌，结合形态学特征及ITS、EF1-α基因序列进行鉴定，通过回接试验确定病原菌。结果显示：共分离得到5种镰刀菌，分别是腐皮镰刀菌（Fusarium solani）、尖孢镰刀菌（F. oxysporum）、芬芳镰刀菌（F. redolens）、F. nygamai和镰状镰刀菌（F. falciforme）。致病性分析表明，这5个菌种均可引起苜蓿根腐病，其中腐皮镰刀菌和尖孢镰刀菌致病能力较强，是导致呼和浩特病害调查区苜蓿根腐病发生严重的主要致病菌。

关键词: 苜蓿, 根腐病, 镰刀菌, 分子鉴定, 致病性

CLC Number:

S435.4

JIA Lei, HE Jie-pu, WU Chong-bin, GUO Yu-han, LIU Xin, SHI Yun, BAI Jia-he, XU Rui, SHEN Hong-yan, ZHAO Ya-ru, DING Wang, CONG Li-li. Isolation and Identification of Pathogens Causing Alfalfa Root Rot in Hohhot and Analysis of Strain Pathogenicity[J]. Acta Agrestia Sinica, 2025, 33(10): 3438-3447.

贾磊, 何介普, 伍崇斌, 郭雨寒, 刘信, 石昀, 白家赫, 徐瑞, 沈宏燕, 赵雅茹, 丁旺, 丛丽丽. 呼和浩特苜蓿根腐病病原菌分离鉴定及菌株致病性分析[J]. 草地学报, 2025, 33(10): 3438-3447.

References

[1] 方香玲，张彩霞，南志标. 紫花苜蓿镰刀菌根腐病研究进展[J]. 草业学报，2019，28（12）:169-183
[2] 孙红霞，史志丹，房永雨，等. 苜蓿根腐病根内细菌的群落结构及多样性[J]. 中国草地学报，2023，45（12）:80-88
[3] HELLEBOIS T，GAIANI C，FORTUIN J，et al. Cryotropic gel-forming capacity of alfalfa （Medicago sativa L.） and fenugreek （Trigonella foenum graecum） seed galactomannans[J]. Carbohydrate Polymers，2021（267）:118190
[4] 梁嘉俊，庞仁江，宁军权，等. 陕西省紫花苜蓿根腐病致病镰孢菌的分离与鉴定[J]. 植物保护学报，2019，46（3）:715-716
[5] 张彩霞，方香玲. 草类植物抗病机制研究进展[J]. 草业学报，2023，32（1）:203-215
[6] 李孟滕，王芯，马晓冉，等. 紫花苜蓿根腐病病原菌分离鉴定与致病性[J]. 中国草地学报，2022，44（11）:84-91
[7] SUN L，DENG L G，FAN J Q，et al. First report of Fusarium commune Causing Bulb Rot on Lily （Lilium brownii var. Viridulum Baker） in Hunan Province of China[J]. Plant Disease，2024，108（7）:2220
[8] 孙红霞. 内蒙古苜蓿根腐病根内生菌群落及苜蓿对根内分离病原菌的抗性评价[D]. 呼和浩特:内蒙古大学，2023:10-11
[9] 支旭欣，张帆，杨青川，等. 紫花苜蓿镰刀菌根腐病抗病种质资源筛选[J]. 中国草地学报，2022，44（4）:104-112
[10] AN C Q，LIU M J，ZHANG Z，et al. Contribution of the alternative pathway on spring rejuvenation of alfalfa[J]. Journal of Plant Physiology，2024 （302）:154319
[11] XIE Z，GAO C，ZHANG X Y，et al. Identification and biological characteristics of Fusarium tobaccum sp，a novel species causing tobacco root rot in Jilin Province，China[J]. Microbiology Spectrum，2024，12（12）:e0092524
[12] 杨剑锋，张园园，王娜，等. 苜蓿根腐病病原菌的分离鉴定及苜蓿品种的抗性评价[J]. 中国草地学报，2020，42（3）:52-60
[13] CHEN L L，XIA F S，WANG M Y，et al. Physiological and proteomic analysis reveals the impact of boron deficiency and surplus on alfalfa （Medicago sativa L.） reproductive organs[J]. Ecotoxicology and Environmental Safety，2021（214）:112083
[14] 丛丽丽，康俊梅，张铁军，等. 苜蓿镰刀菌根腐病病原菌的分离鉴定与致病性分析[J]. 草地学报，2017，25（4）:857-865
[15] BOOTH C. The genus Fusarium[M]. Surrey:CMI，1971:14-21
[16] 张园园，王乐，林克剑，等. 不同紫花苜蓿种质对镰刀菌根腐病的抗病性评价[J]. 中国草地学报，2021，43（10）:98-105
[17] 丛丽丽. 抗镰刀菌根腐病苜蓿品种的鉴定及蛋白质组学研究[D]. 北京:中国农业科学院，2017:53-54
[18] WANG S Z，HAN H B，ZHANG B，et al. Identification of crucial genes and regulatory pathways in alfalfa against Fusarium root rot[J]. Plants，2023，12（20）:3634
[19] ZHU G L，LIU J，WU H，et al. The optimum mixed cropping ratio of oat and alfalfa enhanced plant growth，forage yield，and forage quality in saline soil[J]. Plants，2024，13（21）:3103
[20] WANG J H，YIN M H，DUAN Y Y，et al. Enhancing water and soil resources utilization via wolfberry-alfalfa intercropping[J].Plants，2024，13（17）:2374
[21] 张红玲.内蒙古自治区草莓根腐病病原鉴定及皿内防治药剂筛选[D]. 呼和浩特:内蒙古农业大学，2024:12-15
[22] 杨丽. 抗苜蓿根腐病菌株的分离鉴定及其抗苜蓿尖孢镰刀菌的实验研究[J]. 中国草食动物科学，2021，41（6）:41-44
[23] LI B，ZHENG Y Y，CAI Y N，et al. Identification and assessment of a biocontrol agent，Ochrobactrum intermedium I-5，for management of alfalfa root rot caused by Fusarium tricinctum[J]. Phytopathology，2021，111（11）:1927-1934
[24] 沈会芳，杨祁云，张景欣，等. 烟草镰刀菌根腐病生防菌GDND-2的鉴定及防病效果[J]. 中国农学通报，2024，40（23）:126-134
[25] 张恩天，于铁峰，郝凤，等. 种植密度和施肥量对紫花苜蓿生长特性的影响[J]. 草原与草坪，2024:1-13
[26] LIU L，SI L，ZHANG L S，et al. Metabolomics and transcriptomics analysis revealed the response mechanism of alfalfa to combined cold and saline-alkali stress[J].The Plant Journal，2024，119（4）:1900-1919
[27] 李国良，刘凤歧，曲善民，等. 苜蓿尖孢镰刀菌生物学特性研究[J]. 黑龙江畜牧兽医，2016（7）:142-143
[28] 杨剑锋. 苜蓿根腐病病原菌分离鉴定及不同苜蓿品种对镰刀型根腐病抗性评价[D]. 呼和浩特:内蒙古农业大学，2021:34-35
[29] YANG J，HAN J，JING Y Q，et al. Virulent Fusarium isolates with diverse morphologies show similar invasion and colonization strategies in alfalfa[J]. Frontiers in Plant Science，2024，（15）:1390069
[30] SRINIVASAN R，LALITHA T，BRINTHA N C，et al. Predicting the growth of F. proliferatum and F. culmorum and the growth of mycotoxin using machine learning approach[J]. Biomed Research International，2022，2022（0）:9592365
[31] 杨剑锋，王乐，张园园，等. 呼和浩特市苜蓿根腐病病原菌鉴定及致病性测定[J]. 植物保护学报，2022，49（4）:1093-1101
[32] 刘东霞，杨莉，宋连昭，等. 三种镰孢菌对不同苜蓿品种致病性的比较研究[J]. 草地学报，2022，30（4）:909-918
[33] BERG L E，MILLER S S，DORNBUSCH M R，et al. Seed rot and damping-off of alfalfa in Minnesota caused by Pythium and Fusarium species[J]. Plant Disease，2017，101（11）:1860-1867
[34] AFZALINIA S，MEHRABI-KOUSHKI M，FAROKHINEJAD R. Two new species of Fusarium in the F. incarnatum-equiseti species complex from Oryza sativa in Iran[J]. Antonie Van Leeuwenhoek，2024，118（1）:5
[35] WONGLOM P，SUNPAPAO A. Fusarium incarnatum\n is associated with postharvest fruit rot of muskmelon （\n Cucumis melo\n） [J]. Journal of Phytopathology，2020，168（4）:204-210
[36] 王乐，张园园，王娜，等. 紫花苜蓿12种镰刀菌的菌落生长和产孢条件[J]. 中国草地学报，2023，45（6）:103-116
[37] 张彩霞. 紫花苜蓿根腐病病原镰刀菌多样性及防御机理研究[D]. 兰州:兰州大学，2024:14-15
[38] 王乐，贾瑞芳，王娜，等. 内蒙古自治区紫花苜蓿根腐病病原菌鉴定[J]. 中国草地学报，2024，46（6）:103-116
[39] 杨吉明，王亚杰，马亚男，等. AM真菌与木霉对紫花苜蓿应答尖孢镰刀菌侵染的影响[J]. 草业科学，2024，1（17）:12-24
[40] 陈博，牛艺帆，刘晓宇，等. 腐皮镰刀菌病原致病性及苜蓿品种抗病性研究[J]. 草业学报，2023，32（10）:115-128
[41] LEYVA-M，KARLA Y，CLAUDIA P L，et al. Fusarium species from the Fusarium fujikuroi species complex involved in mixed infections of maize in Northern Sinaloa， Mexico[J]. Journal of Phytopathology，2015，163（6）:486-497
[42] BOZOLU T，DERVI S，IMREN M，et al. Fungal pathogens associated with crown and root rot in wheat-growing areas of Northern Kyrgyzstan[J]. Journal of Fungi，2023，9（1）:124

Isolation and Identification of Pathogens Causing Alfalfa Root Rot in Hohhot and Analysis of Strain Pathogenicity

呼和浩特苜蓿根腐病病原菌分离鉴定及菌株致病性分析

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