Effects of Exogenous Hydrogen Sulfide on Seed Germination and Seedling Growth of Lespedeza davurica under Drought Stress

doi:10.11733/j.issn.1007-0435.2025.04.019

Acta Agrestia Sinica ›› 2025, Vol. 33 ›› Issue (4): 1192-1200.DOI: 10.11733/j.issn.1007-0435.2025.04.019

Effects of Exogenous Hydrogen Sulfide on Seed Germination and Seedling Growth of Lespedeza davurica under Drought Stress

ZHANG Ya-hui^1,2,3, NIU Li^1,2,3, WANG Yong-xin^1,2,3, ZHANG Xiao-yan^1,2,3, ZHANG Tian^1,2,3, XIA Fang-shan^1,2,3, ZHAO Xiang^1,2,3, ZHU Hui-sen^1,2,3

1. College of Grassland Science, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China;
2. Shanxi Key Laboratory of Grassland Ecological Protection and Native Grass Germplasm Innovation, Taigu, Shanxi Province 030801, China;
3. Key Laboratory of Model Innovation in Forage Production Efficiency, Ministry of Agriculture and Rural Affairs, P. R. China, Taigu, Shanxi Province 030801, China

Received:2024-07-12 Revised:2024-08-28 Online:2025-04-15 Published:2025-04-28

外源硫化氢对干旱胁迫下达乌里胡枝子种子萌发及幼苗生长的影响

张亚慧^1,2,3, 牛莉^1,2,3, 王永新^1,2,3, 张晓燕^1,2,3, 张田^1,2,3, 夏方山^1,2,3, 赵祥^1,2,3, 朱慧森^1,2,3

1. 山西农业大学草业学院, 山西太谷 030801;
2. 草地生态保护与乡土草种质创新山西省重点实验室, 山西太谷 030801;
3. 农业农村部饲草高效生产模式创新重点实验室, 山西太谷 030801

通讯作者: 王永新,E-mail:yongxwang@126.com
作者简介:张亚慧（1998-），女，汉族，山西大同人，硕士研究生，主要从事饲草生产与利用，E-mail：zhangyahui202105@163.com
基金资助:
山西省现代农业产业体系建设项目（2023CYJSTX11-11）；山西省重点研发计划项目（202102140601006）资助

Abstract

Abstract: In order to study the effects of exogenous hydrogen sulfide on seed germination and seedling growth of Lespedeza davurica under drought stress，Lespedeza davurica seeds under drought stress were used as the research object. Sodium hydrosulfide was adopted as the exogenous donor of hydrogen sulfide. PEG-6000 was used to simulate drought stress in this experiment. After soaking with exogenous hydrogen sulfide of different concentrations， seed germination indexes and the physiological and growth indexes of seedling were measured. The results indicated that seed soaking with exogenous hydrogen sulfide enhanced the antioxidant enzyme activity of seedlings under drought stress， increased relative water content， and reduced the relative conductivity and malondialdehyde content， thereby alleviating the inhibitory effects of drought stress on seed germination and seedling growth， among which 50 μmol·L^-1 exogenous hydrogen sulfide was the most effective. To sum up， seed soaking with exogenous hydrogen sulfide not only boosted seed germination and seedling growth， but also strengthened its drought resistance.

Key words: Lespedeza davurica, Hydrogen sulfide, Drought stress, Antioxidant enzyme activity

摘要： 为探讨外源硫化氢（H₂S）对干旱胁迫下达乌里胡枝子（Lespedeza davurica）种子萌发及幼苗生长的影响，本试验以干旱胁迫下的达乌里胡枝子种子为研究对象，硫氢化钠为H₂S的外源供体，采用PEG-6000模拟干旱胁迫，经不同浓度的外源H₂S浸种后，测定种子萌发和幼苗的生长生理指标。结果表明：外源H₂S浸种提高了干旱胁迫下幼苗的抗氧化酶活性，增加了幼苗的相对含水量，同时降低了幼苗的相对电导率以及丙二醛含量，从而缓减了干旱胁迫对达乌里胡枝子种子萌发和幼苗生长的抑制作用，且以50 μmol·L^-1的外源H₂S的浸种效果最佳。综上所述，外源H₂S浸种促进了达乌里胡枝子种子萌发和幼苗生长并增强了其抗旱性。

关键词: 达乌里胡枝子, 硫化氢, 干旱胁迫, 抗氧化酶活性

CLC Number:

S812.2

ZHANG Ya-hui, NIU Li, WANG Yong-xin, ZHANG Xiao-yan, ZHANG Tian, XIA Fang-shan, ZHAO Xiang, ZHU Hui-sen. Effects of Exogenous Hydrogen Sulfide on Seed Germination and Seedling Growth of Lespedeza davurica under Drought Stress[J]. Acta Agrestia Sinica, 2025, 33(4): 1192-1200.

张亚慧, 牛莉, 王永新, 张晓燕, 张田, 夏方山, 赵祥, 朱慧森. 外源硫化氢对干旱胁迫下达乌里胡枝子种子萌发及幼苗生长的影响[J]. 草地学报, 2025, 33(4): 1192-1200.

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Effects of Exogenous Hydrogen Sulfide on Seed Germination and Seedling Growth of Lespedeza davurica under Drought Stress

外源硫化氢对干旱胁迫下达乌里胡枝子种子萌发及幼苗生长的影响

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