Whole Genome Analysis of Alternaria Leaf SpotPathogenfrom Silage Maize andDistribution of Toxin Genes Among Different Strains

doi:10.11733/j.issn.1007-0435.2025.08.003

Acta Agrestia Sinica ›› 2025, Vol. 33 ›› Issue (8): 2433-2442.DOI: 10.11733/j.issn.1007-0435.2025.08.003

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Whole Genome Analysis of Alternaria Leaf SpotPathogenfrom Silage Maize andDistribution of Toxin Genes Among Different Strains

LIU Zhong-ge, CHANG Jian-ping, QI He-xing

College of Agriculture and Animal Husbandry, Qinghai University, Xining, Qinghai Province 810016, China

Received:2024-08-13 Revised:2024-10-19 Online:2025-08-15 Published:2025-08-27

青贮玉米链格孢叶斑病菌全基因组解析及不同菌株中毒素合成基因的分布

刘钟歌, 常建萍, 祁鹤兴

青海大学农牧学院, 青海西宁 810016

通讯作者: 祁鹤兴, E-mail:qhx390495559@126.com
作者简介:刘钟歌(2003-),女,汉族,湖南常德人,本科生,主要从事植物病理研究,E-mail:3296871059@qq.com
基金资助:
国家自然科学基金（32460653）资助

Abstract

Abstract: The leaf spot of silage maize caused by Alternaria alternata is one of the important diseases threatening the health of silage maize （Zea mays L.） in Qinghai province. To deeply analyze the genomic characteristics of the Alternaria alternata strain DT18131-B and the distribution of its toxin genes， this study conducted whole-genome sequencing of DT18131-B using the Illumina PE150 sequencing platform and analyzed it with bioinformatics techniques. The results showed that the genome size of the strain was 34.07 Mb， encoding 9578 genes， of which 9519 genes were annotated in the Non-redundant protein database （NR）. Additionally， 818 secreted proteins and 28 secondary metabolite gene clusters were identified. This study focused on the distribution of 7 polyketide synthase （PKS） toxin genes among 90 strains of Alternaria alternata from Qinghai Province. Through cluster analysis of 90 strains， it found that there were genetic relationships and gene diversity among strains in different regions. This study provides important information for understanding the pathogenic mechanism of Alternaria alternata and developing the prevention and control strategy of silage maize leaf spot.

Key words: Silage maize, Alternaria leaf spot, Genome, Bioinformatics, Mycotoxins

摘要： 交链格孢（Alternaria alternata）引起的青贮玉米（Zea mays L.）链格孢叶斑病是威胁青海省青贮玉米健康的重要病害之一。为深入解析交链格孢菌株DT18131-B的基因组特征及其毒素合成基因的分布，本研究通过Illumina PE150测序平台对青贮玉米链格孢叶斑病菌株DT18131-B进行全基因组测序，并利用生物信息学技术进行分析。结果显示，该菌株基因组大小为34.07 Mb，编码9578个基因，其中9519个基因在非冗余蛋白质数据库（Non-redundant protein database，NR）中得到注释，并发现菌株具有818个分泌蛋白和28个次级代谢产物基因簇。论文重点分析了7个聚酮合酶（PKS）毒素合成基因在90株青海省玉米链格孢叶斑病菌交链格孢菌中的分布，通过对这90株交链格孢菌株的聚类分析发现不同地区菌株间存在亲缘关系和基因多样性。本研究为理解交链格孢菌的致病机制和开发青贮玉米叶斑病的防控策略提供了重要信息。

关键词: 青贮玉米, 链格孢叶斑病, 全基因组, 生物信息学, 真菌毒素

CLC Number:

S435.4

LIU Zhong-ge, CHANG Jian-ping, QI He-xing. Whole Genome Analysis of Alternaria Leaf SpotPathogenfrom Silage Maize andDistribution of Toxin Genes Among Different Strains[J]. Acta Agrestia Sinica, 2025, 33(8): 2433-2442.

刘钟歌, 常建萍, 祁鹤兴. 青贮玉米链格孢叶斑病菌全基因组解析及不同菌株中毒素合成基因的分布[J]. 草地学报, 2025, 33(8): 2433-2442.

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Whole Genome Analysis of Alternaria Leaf SpotPathogenfrom Silage Maize andDistribution of Toxin Genes Among Different Strains

青贮玉米链格孢叶斑病菌全基因组解析及不同菌株中毒素合成基因的分布

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