The Effect of Symbiosis between Endophytic Fungi and Hordeum Bogdanii on Nutrients and Enzyme Activities in Rhizosphere and Non-rhizosphere Soils

doi:10.11733/j.issn.1007-0435.2026.01.003

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (1): 23-32.DOI: 10.11733/j.issn.1007-0435.2026.01.003

The Effect of Symbiosis between Endophytic Fungi and Hordeum Bogdanii on Nutrients and Enzyme Activities in Rhizosphere and Non-rhizosphere Soils

XU Hong-e¹, HU Meng-fei¹, ZHANG Bo-yang¹, YANG Rui¹, WANG Dong², CHEN Shui-hong¹

1. College of Life Science and Technology, Tarim University/Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin, Alar, Xinjiang 843300, China;
2. School of Animal Science and Technology, Tarim University, Alar, Xinjiang 843300, China

Received:2024-12-25 Revised:2025-03-26 Published:2025-12-24

内生真菌与布顿大麦共生对根际与非根际土壤养分和酶活性的影响

徐洪娥¹, 户萌菲¹, 张博阳¹, 杨瑞¹, 王栋², 陈水红¹

1. 塔里木大学生命科学与技术学院/塔里木盆地生物资源保护利用兵团重点实验室, 新疆阿拉尔 843300;
2. 塔里木大学动物科学与技术学院, 新疆阿拉尔 843300

通讯作者: 陈水红,E-mail:cshdky@126.com
作者简介:徐洪娥（2000-），女，汉族，贵州毕节人，硕士研究生，主要从事真菌抗逆研究，E-mail:2094932591@qq.com；
基金资助:
内生真菌调控布顿大麦光合色素合成机理（32260356）；塔里木大学校长基金项目（TDZKZD202201）；青藏高原种质资源研究与利用实验室开放课题项目（2023SYS01）资助

Abstract

Abstract: In order to explore the effects of symbiosis between endophytic fungi and Hordeum bogdanii on nutrient contents and enzyme activities in rhizosphere and non-rhizosphere soils， Hordeum bogdanii with Epichloë endophytic fungi （E+） and without Epichloë endophytic fungi （E-） were planted in two fields in Wensu County， Xinjiang in this experiment. The contents of total phosphorus， available phosphorus， total potassium， available potassium， total nitrogen and organic carbon in rhizosphere and non-rhizosphere soils and the activities of soil urease， invertase， catalase and alkaline phosphatase were determined. The results showed that the contents of available phosphorus， total potassium， available potassium and organic carbon， and the activities of urease， sucrase， catalase and alkaline phosphatase in E+ and E- rhizosphere soils were significantly higher than those in non-rhizosphere soils. The correlation analysis of soil nutrients and enzyme activities showed that the activities of urease， sucrase and alkaline phosphatase in rhizosphere soil were significantly positively correlated with soil total phosphorus and organic carbon content. In non-rhizosphere soil， soil urease， invertase and alkaline phosphatase activities were significantly positively correlated with organic carbon. In addition， soil nutrient and enzyme activity redundancy analysis showed that organic carbon was the most important factor affecting rhizosphere soil enzyme activity. The above results showed that the symbiosis of endophytic fungi and H. bogdanii could improve the rhizosphere soil nutrient contents and enhance the rhizosphere soil enzyme activities， thereby improving soil quality and providing new ideas and scientific basis for soil improvement.

Key words: Endophytic fungi, Hordeum bogdanii, Rhizosphere and non-rhizosphere soils, Soil nutrients, Soil enzyme activity

摘要： 为了探究内生真菌与布顿大麦（Hordeum bogdanii）共生对根际和非根际土壤养分含量和酶活性的影响。本试验以新疆温宿县携带Epichloë内生真菌（E+）与不携带内生真菌（E-）的布顿大麦为材料，种植在2块大田中，测定其根际和非根际土壤全磷、效磷、全钾、速效钾、全氮、和有机碳等养分含量和土壤脲酶、蔗糖酶、过氧化氢酶和碱性磷酸酶活性。结果表明：E+、E-根际土壤速效磷、全钾、速效钾和有机碳等养分含量和土壤脲酶、蔗糖酶、过氧化氢酶和碱性磷酸酶性均显著高于非根际土壤。在低盐条件下，E+根际土壤速效磷含量和土壤过氧化氢酶、碱性磷酸酶和蔗糖酶活性均显著高于E-；在高盐条件下，E+根际土壤全磷、速效磷和速效钾养分含量和脲酶活性显著高于E-。土壤养分和酶活性相关性分析表明，根际土壤脲酶、蔗糖酶和碱性磷酸酶活性与土壤全磷和有机碳含量极显著正相关；非根际土壤中，土壤脲酶、蔗糖酶和碱性磷酸酶活性与有机碳呈显著正相关。此外，土壤养分和酶活性冗余分析表明，有机碳是影响根际土壤酶活性最主要的因素。以上结果表明，内生真菌与布顿大麦共生可以提高根际土壤养分含量和增强根际土壤酶活性，从而改善土壤质量，为土壤改良提供新思路和科学依据。

关键词: 内生真菌, 布顿大麦, 根际和非根际土壤, 土壤养分, 土壤酶活性

CLC Number:

S154

XU Hong-e, HU Meng-fei, ZHANG Bo-yang, YANG Rui, WANG Dong, CHEN Shui-hong. The Effect of Symbiosis between Endophytic Fungi and Hordeum Bogdanii on Nutrients and Enzyme Activities in Rhizosphere and Non-rhizosphere Soils[J]. Acta Agrestia Sinica, 2026, 34(1): 23-32.

徐洪娥, 户萌菲, 张博阳, 杨瑞, 王栋, 陈水红. 内生真菌与布顿大麦共生对根际与非根际土壤养分和酶活性的影响[J]. 草地学报, 2026, 34(1): 23-32.

References

[1] GONG X F，ZHAO Y，PENG Y N，et al. Cultivation effect on improving saline-alkai wasteland soil in tianshan moutain of xinjiang[J]. Chinese Journal of Soil Science，2024，55（3）：622-633 巩晓芳，赵毅，彭轶楠，等. 新疆天山盐碱荒地土壤开垦种植效应分析[J]. 土壤通报，2024，55（3）：622-633
[2] ZHNAG X Q，LI Z F，ZHANG T Q，et al.Nutrient characteristics of rhizosphere and non-rhizosphere soil of chinese prickly ash with root rot disease[J]. Shaanxi Forest Science and Technology，2023，51（2）：12-17 张雪倩，李子菲，张添棋，等. 根腐病花椒根际和非根际土壤养分特征研究[J]. 陕西林业科技， 2023，51（2）：12-17
[3] YANG X W，LIU J，HOU M Y，et al. Enzyme activities and stoichiometric characteristics of rhizometric and nonrhizosphere soil in different ancient tea gardens in Fengqing County， China[J]. Chinese Journal of Applied and Environmental Biology，2023，29（6）：1418-1425 杨雄伟，刘娇，侯孟月，等. 凤庆县不同古茶园根际与非根际土壤酶活性及其化学计量特征[J]. 应用与环境生物学报， 2023，29（6）：1418-1425
[4] LIAO Y X，SHU Y G，WANG C M，et al. Change characteristics of soil microbial phosphorus，alkaline phosphatase and phytase under different vegetation types in karst area[J]. Journal of Southern Agriculture，2023，54（6）：1762-1770 廖远行，舒英格，王昌敏，等. 喀斯特地区不同植被类型土壤微生物量磷、碱性磷酸酶及植酸酶的变化特征[J]. 南方农业学报， 2023，54（6）：1762-1770
[5] ZAN K Z，A S J，YI M N J，et al. Physicochemical properties and stoichiometric characteristics of rhizosphere and bulk soils of alfalfa of different fall dormancy types[J]. Acta Agrestia Sinica，2022，30（4）：957-965 昝看卓，阿山江·伊米尼江，刘丛，等. 不同秋眠类型紫花苜蓿根际与非根际土壤理化性质及化学计量特征[J]. 草地学报，2022，30（4）：957-965
[6] ZOU Y， ZHANG Y， TESTERINK C. Root dynamic growth strategies in response to salinity[J]. Plant Cell Environment，2022，45（3）：695-704
[7] CHEN Y C，LI X，CAI Y D，et al. Physicochemical properties and fungal community characteristics of dry-cultivated alfalfa rhizosphere and non-rhizosphere soils in the sandy area of Kunlun Mountains[J]. Pratacultural Science，2023，40（5）：1232-1242 陈永成，李肖，蔡宜东，等. 昆仑山沙区旱作紫花苜蓿根际/非根际土壤理化性质及真菌群落特征[J]. 草业科学， 2023，40（5）：1232-1242
[8] YU T Z，ZHANG T Y，ZHOU L Y. Changes of enzyme activities in rhizosphere and non- rhizosphere soil of Cenchrus pauciflorus at different growth stages under substitution competition[J]. Grassland and Turf，2022，42（1）：96-101 于童洲，张天宇，周立业. 少花蒺藜草与不同牧草混播根际与非根际土壤酶活性变化[J]. 草原与草坪， 2022，42（1）：96-101
[9] HU M F，CHEN S，LIU X Z，et al. Effects of endophytic fungi expansion of Hordeum bogdanii on soil enzyme activity and nutrients[J]. Journal of Biology，2024，41（4）：57-64 户萌菲，陈胜，刘小珍，等. 布顿大麦内生真菌扩张对土壤酶活和养分的影响[J]. 生物学杂志， 2024，41（4）：57-64
[10] LIU R G，DENG M H，ZHANG H W，et al. Effects of epichloë gansuensis endophyte on soil enzyme activities and nutrients at different growth periods of Achnatherum inebrians[J]. Acta Agrestia Sinica，2024，32（6）：1770-1778 刘荣贵，邓茂桦，张瀚文，等. 内生真菌对醉马草不同生长期土壤酶活性和养分的影响[J]. 草地学报. 2024，32（6）：1770-1778
[11] LONG F， HU M F， CHEN S，et al. Endophytic fungi regulate HbNHX1 expression and ion balance in Hordeum bogdanii under alkaline stress[J]. Journal of Fungi，2023，9（3）：331
[12] LIU H，CHEN T，XIA C. On the response of plant-soil feedback to grass endophytes[J]. Pratacultural Science，2020，37（1）：65-74 刘欢，陈焘，夏超. 植物-土壤反馈对禾草内生真菌响应[J]. 草业科学，2020，37（1）：65-74
[13] LEE K， MISSAOUI A， MAHMUD K，et al. Interaction between grasses and Epichloë endophytes and its significance to biotic and abiotic stress tolerance and the rhizosphere[J]. Microorganisms，2021，9（11）2184
[14] HALIFU S Y R M， DENG X， SONG X S，et al. Research Progress in the Working Mechanism of Ectomycorrhizal Fungi for PlantGrowth Promotion and Stress Resistance[J]. World Forestry Research，2021，34（1）：19-24 赛牙热木·哈力甫，邓勋，宋小双，等. 外生菌根真菌对植物促生抗逆作用机制研究进展[J]. 世界林业研究， 2021，34（1）：19-24
[15] LI C J，YAO X，NAN Z B. Advances in research of Achnatherum inebrians-Epichloë endophyte symbionts[J]. Chinese Journal of Plant Ecology，2018，42（8）：793-805 李春杰，姚祥，南志标. 醉马草内生真菌共生体研究进展[J]. 植物生态学报， 2018，42（8）：793-805
[16] WANG K，LIN L D，LONG F，et al. Effect of endophytic fungi on fiber and crude fat content of Hordeum bogdanii at fruiting stage under alkali stress[J]. Heilongjiang Animal Science and Veterinary Medicine，2022（9）：99-104 王凯，林利栋，龙凤，等. 碱胁迫下内生真菌对结实期布顿大麦纤维和粗脂肪含量的影响[J]. 黑龙江畜牧兽医， 2022（9）：99-104
[17] NAN Z B.Effects of endophytic fungi on the growth of Hordeum bogdanii[J]. Pratacultural Science，1996（1）：16-18 南志标. 内生真菌对布顿大麦草生长的影响[J]. 草业科学， 1996（1）：16-18
[18] LONG F， HU M F， CHEN S，et al. Effects of endophytic fungi on the anatomical structure of the host Hordeum bogdanii under alkali stress[J]. Acta Agrestia Sinica，2023，31（2）：358-364 龙凤，户萌菲，陈胜，等. 碱胁迫下内生真菌对宿主布顿大麦显微结构的影响[J]. 草地学报， 2023，31（2）：358-364
[19] LONG F. Epichloë bromicola Effects of endophytic fungi on growth and ionbalance of Hordeum bogdanii under alkaline stress[J]. Aral：TarimUniversity，2023：14-19
[20] HAN D，LONG F，CHEN S，et al.Cloning of TB1 gene in Hordeum bogdanii and the effect of endophytic fungi on its expression level[J]. Acta Prataculturae Sinica，2023，32（8）：176-185 韩丹，龙凤，陈胜，等. 布顿大麦TB1基因的克隆及内生真菌对其表达量的影响[J]. 草业学报， 2023，32（8）：176-185
[21] ZHANG Y，ZHANG C H，WANG Q T，et al. Difference of soil carbon sequestration between rhizosphere and bulk soil in a mountain coniferous forest in southwestern China under nitrogen depositions[J]. Chinese Journal of Plant Ecology，2023，47（9）：1234-1244 张英，张常洪，汪其同，等. 氮沉降下西南山地针叶林根际和非根际土壤固碳贡献差异[J]. 植物生态学报， 2023，47（9）：1234-1244
[22] ZHAO F C，CHEN H F，WANG Y H，et al. Response of rhizosphere soil properties to changed precipitation and nitrogen addition in a salinized grassland[J]. Acta Agrestia Sinica，2022，30（9）：2430-2437 赵芳草，陈鸿飞，王一昊，等. 盐渍化草地根际土壤理化性质对降水改变和氮添加的响应[J]. 草地学报， 2022，30（9）：2430-2437
[23] XIE S X. One of the methods for determining saline-alkali soil： Determination of total soluble salts in soil[J]. Soil and Fertilizer Sciences，1975（1）：57-59 谢森祥. 盐碱土测定方法之一土壤可溶盐总量的测定[J]. 土壤肥料， 1975（1）：57-59
[24] QIAN S P. Discussion on the determination methods of total phosphorus in soil[J]. Xinjiang Agricultural Science and Technology，2001（4）：24-25 钱淑萍. 土壤全磷测定方法讨论[J]. 新疆农业科技， 2001（4）：24-25
[25] LU R K. Analytical methods for soil agrochemistry[M]. Beijing：China Agriculture Press，2000：638 鲁如坤. 土壤农业化学分析方法[M]. 北京：中国农业科技出版社， 2000：638
[26] YAO X. Effects of Achnatherum inebrians-Epichloëgansuensis symbiont on plant compositionand soil microbial community diversity inrangelands[D]. Lanzhou：Lanzhou University，2019：11-19 姚祥. 醉马草内生真菌共生体对草地植物组成及土壤微生物群落多样性的影响[D]. 兰州：兰州大学， 2019：11-19
[27] GUANG S Y. Soil enzymes and their research methods[M]. Beijing：Agriculture Press，1986：376 关松荫. 土壤酶及其研究法[M]. 北京：农业出版社， 1986：376
[28] LONG F， HU M， CHEN S， et al. Endophytic fungi regulate HbNHX1 expression and ion balance in Hordeum bogdanii under Alkaline Stress[J]. Journal of Fungi. 2023，9（3）：331
[29] YANG Y，LIU B R. Distribution of soil nutrient and microbial biomass in rhizosphere versus nonrhizosphere area of different plant species in desertified steppe[J]. Acta Ecologica Sinica，2015，35（22）：7562-7570 杨阳，刘秉儒. 荒漠草原不同植物根际与非根际土壤养分及微生物量分布特征[J]. 生态学报， 2015，35（22）：7562-7570
[30] WANG Y S， JENSEN C R， LIU F L. Nutritional responses to soil drying and rewetting cycles under partial root-zone drying irrigation[J]. Agricultural water management. 2017， 179： 254-259
[31] SHENG W Q. Effects of glyphosate on soil enzyme activities in eucalyptus forest land[J]. Modern Agricultural Science and Technology，2023（15）：160-162 笙万庆. 草甘膦对桉树林地土壤酶活性的影响[J]. 现代农业科技， 2023（15）：160-162
[32] ZHAN S F，LU D N，MAO H Y，et al. Isolation of two phosphate/potaaium-solubilizing and IAA-producing strains of endophytic fungi and their plant growth promoting function[J]. Soil and Fertilizer Sciences in China，2017（3）：142-151 詹寿发，卢丹妮，毛花英，等. 2株溶磷、解钾与产IAA的内生真菌菌株的筛选、鉴定及促生作用研究[J]. 中国土壤与肥料， 2017（3）：142-151
[33] CHEN M H，MO M H，MA L，et al. Screening of phosphate-solubilizing fungi from phosphate-rich soils in Dianchi and its effect on growth promoting in Brassica napus[J]. Transactions of the Chinese Society of Agricultural Engineering，2012，28（Sup1）：209-215 陈明会，莫明和，马莉，等. 滇池富磷区土壤中溶磷真菌的筛选及其对油菜的促生作用[J]. 农业工程学报， 2012，28（Sup1）：209-215
[34] WANG L D，WANG F L，GUO C X，et al. Progress of soil enzymology[J]. Soils，2016，48（1）：12-21 王理德，王方琳，郭春秀，等. 土壤酶学硏究进展[J]. 土壤， 2016，48（1）：12-21
[35] PENG N. Analysis of soil enzyme activity and its influencing factors in different vegetanion types in Helan Mountains[D].Yinchuan：Ningxia University， 2023：13-18 彭妞. 贺兰山不同植被类型土壤酶活性及其影响因素分析[D]. 银川：宁夏大学， 2023：13-18
[36] WANG S J，GUO Y C，ZENG L S，et al.Effects of suaeda planting rate on soil enzyme activity and aggregation of coastal saline alkali land[J]. Journal of Agricultural Science and Technology， 2021，23（12）：179-185 王思霁，国艳春，曾路生，等. 碱蓬播种量对滨海盐碱地土壤酶活性和团聚性的影响[J]. 中国农业科技导报，2021，23（12）：179-185
[37] LI L J，XIE T T，ZHANG S L，et al.Characteristics of nutrient content and enzyme activity in the rhizosphere and bulk soils of four suitable plant species in the hydro-fluctuation zone of the Three Gorges Reservoir[J].Acta Ecologica Sinica， 2020，40（21）：7611-7620 李丽娟，谢婷婷，张松林，等. 三峡库区消落带4种适生植物根际与非根际土壤养分与酶活性特征研究[J]. 生态学报， 2020，40（21）：7611-7620
[38] WU L K，LIN X M，LIN W X. Advances and perspective in research on plant-soil-microbe interactions mediated by root exudates[J]. Chinese Journal of Plant Ecology，2014，38（3）：298-310 吴林坤，林向民，林文雄. 根系分泌物介导下植物-土壤-微生物互作关系研究进展与展望[J]. 植物生态学报， 2014，38（3）：298-310
[39] GAO Y.Effects of festuca sinensis-Epichloë endophyte symbionts on growth of Elymusnutans and rhizosphere microorganis[D]. Lanzhou：Lanzhou University， 2023：30-23 高越. 中华羊茅内生真菌共生体对垂穗披碱草生长及根际微生物的影响[D]. 兰州：兰州大学， 2023：30-23
[40] JI X， XIA Y， ZHANG H， et al. The microscopic mechanism between endophytic fungi and host plants： From recognition to building stable mutually beneficial relationships[J]. Microbiological Research，2022， 261：127056
[41] LU P. Effects of long-term fertilization andmanagement on phoD harboring microbialcommunity and alkaline phosphatase onLoess soil[D].Yanglin：Northwest Agriculture and Forestry University， 2023：9-21 路鹏. 长期不同施肥及土地利用对塿土中phoD基因编码的细菌及碱性磷酸酶的影响[D]. 杨凌：西北农林科技大学， 2023：9-21
[42] HOU W J，ZOU M，LI B F，et al. Effect of glyphosate on soil enzyme activities in eucalyptus plantations[J]. Journal of Northeast Forestry University，2020，48（11）：76-79 侯文军，邹明，李宝福，等. 草甘膦对桉树人工林土壤酶活性的影响[J]. 东北林业大学学报. 2020，48（11）：76-79
[43] CHEN Z. Qilian Mountain grassland vegetation-soil nutrients-enzyme stoichiometry characteristics study[D]. Lanzhou： Lanzhou Jiaotong University，2024：31-10 陈卓. 祁连山草地植被-土壤养分-酶化学计量特征研究[D]. 兰州：兰州交通大学， 2024：31-10
[44] WANG S J，WANG L N，SUN J，et al.Effects of exogenous organic carbon on soil fertility and soil enzyme activity of the Jun Jujube orchard in Xinjiang[J]. Journal of Central South University of Forestry Technology，2023，43（4）：134-143 王姝婧，王利娜，孙佳，等. 外源有机碳对新疆骏枣园土壤肥力及土壤酶活性的影响[J]. 中南林业科技大学学报， 2023，43（4）：134-143
[45] SUN H，ZHANG J F，XU H S，et al. Variations of soil microbial community composition and enzyme activities with different salinities on Yuyao coast，Zhejiang，China[J]. Chinese Journal of Applied Ecology，2016，27（10）：3361-3370 孙慧，张建锋，许华森，等. 余姚滨海不同盐碱度土壤微生物群落组成及土壤酶活性的变化[J]. 应用生态学报， 2016，27（10）：3361-3370
[46] MO X，CHEN F J，YOU C， et al.Characteristics and factors of soil enzyme activity for different plant communities in Yellow River Delta[J].Environmental Science，2020，41（2）：895-904 莫雪，陈斐杰，游冲，等. 黄河三角洲不同植物群落土壤酶活性特征及影响因子分析[J]. 环境科学， 2020，41（2）：895-904

The Effect of Symbiosis between Endophytic Fungi and Hordeum Bogdanii on Nutrients and Enzyme Activities in Rhizosphere and Non-rhizosphere Soils

内生真菌与布顿大麦共生对根际与非根际土壤养分和酶活性的影响

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