Identification of the AKR Gene Family in Bermudagrass and Expression Analysis under Salt Stress

doi:10.11733/j.issn.1007-0435.2026.07.004

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (7): 2377-2392.DOI: 10.11733/j.issn.1007-0435.2026.07.004

Identification of the AKR Gene Family in Bermudagrass and Expression Analysis under Salt Stress

XI Shu-yan¹, ZHANG Rui-qi¹, WANG Xian-zhi¹, HU Zhe-wei^1,2, GUO Hai-lin¹, CHEN Rong-rong¹, CHEN Jing-bo¹

1. Jiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing, Jiangsu Province 210014, China;
2. College of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang, Henan Province 471000, China

Received:2025-12-31 Revised:2026-02-10 Published:2026-07-02

狗牙根AKR基因家族鉴定及盐胁迫下的表达分析

奚舒燕¹, 张瑞琦¹, 王贤志¹, 胡哲玮^1,2, 郭海林¹, 陈荣荣¹, 陈静波¹

1. 江苏省中国科学院植物研究所(南京中山植物园)/江苏省植物资源研究与利用重点实验室, 江苏南京 210014;
2. 河南科技大学园艺与植物保护学院, 河南洛阳 471000

通讯作者: 陈荣荣，E-mail:670702crr@163.com;陈静波，E-mail:chenjb19@163.com
作者简介:奚舒燕（2001-），女，汉族，江苏盐城人，硕士研究生，主要从事草类植物耐盐胁迫方面研究，E-mail:18352025320@163.com
基金资助:
江苏省社会发展项目（BE2023849）；国家自然科学基金面上项目（32271758）资助

Abstract

Abstract: Aldo-keto reductase （AKR） is widely present in various organisms and plays key roles in plant growth， development and stress responses. Bermudagrass （Cynodon dactylon （L.） Pers.） exhibits rapid growth and strong comprehensive stress tolerance， making it widely utilized in landscaping， turfgrass for sports fields， ecological restoration and forage production. In this study， a total of 88 members of AKR gene family were identified from the bermudagrass genome， which were clustered into 7 subfamilies and unevenly distributed across the 30 chromosomes of bermudagrass. Analysis of cis-acting elements in the promoter regions indicated that all the members contain varying numbers of light-responsive， hormone-responsive and stress-responsive elements， suggesting that this gene family may be involved in multiple growth， developmental， and stress response processes. Different AKR gene members exhibited distinct expression patterns under different salt treatment durations and in different organs. The overall expression response intensity of genes in roots was generally higher than that in leaves， with a greater number of genes being upregulated， whereas most genes in leaves were downregulated. Among them， CdAKR15， CdAKR30， CdAKR43， CdAKR48， CdAKR72， and CdAKR80 showed significant differences in expression levels under different salt stress durations. This study lays a foundation for further investigation of the response of bermudagrass AKR genes to salt stress.

Key words: Cynodon dactylon, Aldo-keto reductase genes, Bioinformatics analysis, Salt stress, Expression analysis

摘要： 醛酮还原酶（Aldo-keto reductase， AKR）广泛存在于不同生物中，在生长发育、胁迫响应等过程中发挥着关键作用。狗牙根（Cynodon dactylon （L.） Pers.）生长快，综合抗逆性强，在园林绿化、运动场草坪、生态修复和牧草生产中得到广泛应用。本研究从狗牙根基因组中鉴定到了88个AKR基因家族成员，可分为7个亚家族，其不均匀地分布在狗牙根30条染色体上。狗牙根AKR基因家族成员启动子区域均存在数量不等的光响应、激素和逆境响应等顺式作用元件，表明其可能参与多种生长发育及逆境响应过程。不同AKR基因家族成员在不同盐处理时间及不同器官下的表达模式具有差异，根中基因的整体表达响应强度普遍高于叶片，上调表达的基因数量较多，而叶片中多数基因表达下调，其中CdAKR15，CdAKR30，CdAKR43，CdAKR48，CdAKR72和CdAKR80基因在不同盐胁迫时间下的表达量差异显著。本研究为进一步探究狗牙根AKR基因响应盐胁迫奠定了基础。

关键词: 狗牙根, AKR基因家族, 盐胁迫, 生物信息学分析, 表达量分析

CLC Number:

S688.4

XI Shu-yan, ZHANG Rui-qi, WANG Xian-zhi, HU Zhe-wei, GUO Hai-lin, CHEN Rong-rong, CHEN Jing-bo. Identification of the AKR Gene Family in Bermudagrass and Expression Analysis under Salt Stress[J]. Acta Agrestia Sinica, 2026, 34(7): 2377-2392.

奚舒燕, 张瑞琦, 王贤志, 胡哲玮, 郭海林, 陈荣荣, 陈静波. 狗牙根AKR基因家族鉴定及盐胁迫下的表达分析[J]. 草地学报, 2026, 34(7): 2377-2392.

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Identification of the AKR Gene Family in Bermudagrass and Expression Analysis under Salt Stress

狗牙根AKR基因家族鉴定及盐胁迫下的表达分析

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