Effects of Complex Salt and Alkali Conditions on Seed Germination and Seedling Chlorophyll Fluorescence Characteristics of Alfalfa

doi:10.11733/j.issn.1007-0435.2013.03.014

›› 2013, Vol. 21 ›› Issue (3): 501-507.DOI: 10.11733/j.issn.1007-0435.2013.03.014

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Effects of Complex Salt and Alkali Conditions on Seed Germination and Seedling Chlorophyll Fluorescence Characteristics of Alfalfa

LIU Xiao-jing, ZHANG Xiao-lei, QI Min-xing, LIU Yan-nan

College of Grassland Science, Gansu Agricultural University/Key Laboratory of Grassland Ecosystem (Gansu Agricultural University), Ministry of Education/Sino-U.S. Centers for Grazingland Ecosystem Sustaiability, Lanzhou, Gansu Province 730070, China

Received:2012-11-28 Revised:2013-01-05 Online:2013-06-15 Published:2013-06-05

混合盐碱对紫花苜蓿种子萌发及幼苗期叶绿素荧光特性的影响

刘晓静, 张晓磊, 齐敏兴, 刘艳楠

甘肃农业大学草业学院草业生态系统教育部重点实验室中-美草地畜牧业可持续发展研究中心, 甘肃兰州 730070

作者简介:刘晓静(1968- ),女,甘肃酒泉人,博士,教授,研究方向为草坪及牧草营养与生理,E-mail:liuxj@gsau.edu.cn
基金资助:
甘肃省自然基金项目"甘肃省秦王川灌区苜蓿草地施肥制度及其对环境的影响研究"(1010RJZA157);草业生态系统教育部重点实验室(甘肃农业大学)项目"供氮水平及氮素形态对紫花苜蓿养分吸收及根瘤特性的影响研究"(CYZS-2011012);甘肃省财政厅项目"外源氮素供应对紫花苜蓿产量和品质形成影响研究"资助

Abstract

Abstract: Seeds and young seedlings of alfalfa (Medicago sativa) were stressed with 20 different alkali-saline conditions simulating salt-alkalinized soil obtained by mixing two neutral salts (NaCl and Na₂SO₄) and two alkaline salts (NaHCO₃ and Na₂CO₃) in various proportions. Under these conditions, the germination rate, germination viability, germination process, photochemical efficiency of PSⅡ (F_v/F_m), potential activity of PSⅡ (F_v/F_o) and chlorophyll content were determined. Results showed that except group A, salt had a negative effect on the germination rate and germination viability of seeds. In the same salt concentrations, the germination process was postponed with increased proportions of alkali salt. As the stress increased, the chlorophyll content increased initially then decreased, F_v/F_m, F_v/F_o were significantly decreased.

Key words: Alfalfa (Medicago sativa), Salt and alkali mixed stress, Seed germination, Chlorophyll, Chlorophyll fluorescence

摘要： 采用2种中性盐NaCl和Na₂SO₄及2种碱性盐NaHCO₃和Na₂CO₃按不同比例混合成A,B,C,D,E共5个组合,设25,50,100,150 mmol·L^-1共4个浓度梯度,模拟出20种不同盐度和碱度的混合盐碱条件处理紫花苜蓿(Medicago sativa)种子,测定其发芽率、发芽势和发芽进程,及沙培条件下混合盐碱胁迫对其幼苗叶绿素含量和叶绿素荧光特性的影响,旨在探讨混合盐碱胁迫对紫花苜蓿种子萌发和幼苗光合特性的影响。结果表明:除A组外,其他各组的发芽率、发芽势均随着盐浓度的升高而降低;同一浓度下随着碱性盐比例的增加种子萌发进程延缓越明显;随着盐浓度的升高,叶绿素含量呈先增加后减少的趋势,荧光参数PSⅡ最大光化学效率(F_v/F_m)和PSⅡ的潜在活性(F_v/F_o)均呈显著降低趋势(P<0.05),不同组合之间表现略有差异。

关键词: 紫花苜蓿, 混合盐碱胁迫, 种子萌发, 叶绿素, 叶绿素荧光

CLC Number:

LIU Xiao-jing, ZHANG Xiao-lei, QI Min-xing, LIU Yan-nan. Effects of Complex Salt and Alkali Conditions on Seed Germination and Seedling Chlorophyll Fluorescence Characteristics of Alfalfa[J]. , 2013, 21(3): 501-507.

刘晓静, 张晓磊, 齐敏兴, 刘艳楠. 混合盐碱对紫花苜蓿种子萌发及幼苗期叶绿素荧光特性的影响[J]. , 2013, 21(3): 501-507.

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Effects of Complex Salt and Alkali Conditions on Seed Germination and Seedling Chlorophyll Fluorescence Characteristics of Alfalfa

混合盐碱对紫花苜蓿种子萌发及幼苗期叶绿素荧光特性的影响

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