Acta Agrestia Sinica ›› 2014, Vol. 22 ›› Issue (4): 822-827.DOI: 10.11733/j.issn.1007-0435.2014.04.022

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Identification and Determination of Biological Characteristics of Endophytic Bacteria 265ZY3 from Stipa capillata in the Alpine Grasslands

WANG Yu-qin, XUE Li, YANG Cheng-de, WANG Ying, YAO Yu-ling, CHEN Xiu-rong   

  1. College of Grassland, Gansu Agricultural University; Key Laboratory of Grassland Ecosystem (Gansu Agricultural University), Ministry of Education; Pratacultural Engineering Laboratory of Gansu Province; Sino-U.S. Center for Grazingland Ecosystem Sustainability, Lanzhou, Gansu Province 730070, China
  • Received:2013-10-24 Revised:2013-11-13 Online:2014-08-15 Published:2014-08-04

高寒草地针茅内生菌265ZY3的生物学特性研究及其鉴定

王玉琴, 薛莉, 杨成德, 王颖, 姚玉玲, 陈秀蓉   

  1. 甘肃农业大学草业学院 草业生态系统教育部重点实验室 甘肃省草业工程实验室 中-美草地畜牧业可持续发展研究中心, 甘肃 兰州 730070
  • 通讯作者: 杨成德
  • 作者简介:王玉琴(1988-),女,甘肃兰州人,硕士研究生,主要从事植物病理学方面的研究,E-mail:gsndwangyuqin@126.com
  • 基金资助:

    国家自然科学基金(31160122);草业生态系统教育部重点实验室开放课题(CYzs-2011011)资助

Abstract:

Endophytic bacterium 265ZY3 was isolated from the Stipa capillata in the alpine meadow. The strain was identified by 16S rDNA sequence analysis. The biological characteristics of the endophytic bacterium such as antibacterial spectrum, IAA secretion, phosphate solubilization and nitrogen fixation were investigated. Results showed that the inhibitive rates of the identified strain to Phoma foveata, Colletotrichum coccodes and Fusarium avenaceum were 81.65%, 87.84% and 72.64%, respectively. The concentration of IAA secreted by 265ZY3 were 42.33 mg·L-1 and 38.59 mg·L-1 in the King medium with 100 mg·L-1 tryptophan and without tryptophan, respectively. The 265ZY3 strain dissolved inorganic phosphorus and organic phosphorus. Moreover, the dissolving capacity of the strain for inorganic phosphorus was about 68 times higher than for organic phosphorus. Meanwhile, the strain had the ability of nitrogen fixation. The strain was identified as Bacillus amyloliquefaciens by the analyses of both 16S rDNA sequence homology and morphological characteristics.

Key words: Stipa, Endophytic bacteria, Biological functions, Identification

摘要:

对高寒草地针茅(Stipa capillata L.)内生细菌265ZY3进行了抗菌、产IAA、溶磷、固氮等生物学特性的研究,并通过16SrDNA序列分析对其进行了鉴定。结果表明:265ZY3对马铃薯坏疽病菌(Phomafoveata)、马铃薯炭疽病菌(Colletotrichum coccodes)和马铃薯枯萎病菌(Fusarium avenaceum)均有良好的抑制作用,抑菌率分别为81.65%,87.84%和72.64%;在不含色氨酸和含100mg·L-1色氨酸的King培养液中分泌IAA的量分别为38.59mg·L-1和42.33mg·L-1;能溶解无机磷和有机磷,且溶解无机磷的能力是有机磷的68倍;同时,具有固氮能力。结合形态学特征,通过16SrDNA序列同源性分析,将265ZY3初步鉴定为解淀粉芽孢杆菌(Bacillus amyloliquefaciens)。

关键词: 针茅, 内生细菌, 生物学特性, 鉴定

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