Acta Agrestia Sinica

Acta Agrestia Sinica ›› 2016, Vol. 24 ›› Issue (1): 218-221.DOI: 10.11733/j.issn.1007-0435.2016.01.031

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Isolation and Screening of Phosphate Solubilizing Rhizobia of Sainfoin

LI Jian-feng1,2, ZHANG Shu-qing1,2, SHI Shang-li2, DU Jian-xiong3, LI Li-xia1   

  1. 1. Guizhou Normal College, Institute of Soil and Environment Bioremediation in Karst habitats, Guiyang, Guizhou Province 550018, China;
    2. Gansu Agriculture University, Key Laboratory of Grassland Ecosystem of Ministry of Education, Lanzhou, Gansu Province 730070, China;
    3. Guizhou University of Finance and Economics, Guiyang, Guizhou Province 550025, China
  • Received:2014-08-26 Revised:2015-06-11 Online:2016-02-15 Published:2016-04-26

溶磷红豆草根瘤菌的分离和筛选

李剑峰1,2, 张淑卿1,2, 师尚礼2, 杜建雄3, 李丽霞1   

  1. 1. 贵州师范学院 喀斯特生境土壤与环境生物修复研究所, 贵州 贵阳 550018;
    2. 甘肃农业大学 草业生态系统教育部重点实验室, 甘肃 兰州 730070;
    3. 贵州财经大学, 贵州 贵阳 550025
  • 通讯作者: 杜建雄
  • 作者简介:李剑峰(1979-),男,甘肃天水人,博士,副教授,研究方向为植物促生菌与植物抗逆生理,E-mail:alexclover@foxmail.com
  • 基金资助:

    国家自然科学基金项目(31300389,41561072);贵州省科技基金项目(黔科合J字[2014]2137);贵州省科技基金项目(黔科合J字[2014]2136)资助

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Abstract:

In order to obtain phosphate solubilizing Rhizobium strains of sainfoin (Onobrychis viciaefolia). Winogradsky N-free medium and PKO medium were used to isolate and screen Rhizobium strains from sainfoin root nodules. By analyzing the capabilities of sparingly inorganic phosphorus solubilizing, IAA secreting and the nodule nitrogenase activity of screened strains, three rhizobia strains RS-1, RS-8 and RS-14 were obtained. And the phosphate solubilizing capability of RS-1 strain reached 6.98 mg·L-1·d-1 in 14 days, and RS-1 with higher IAA secreting capability and could survive at pH values ranging from 4 to 12,and salt tolerance capability at 11%. Results showed that the screening method used was simple and effective, and the isolated strains could have good prospect of application.

Key words: Onobrychis viciaefolia, Rhizobium, Nitrogen fixation, Phosphorus solubilizing, IAA

摘要：

为获得溶磷红豆草(Onobrychis viciae folia)根瘤菌(Rhizobium sp.),本研究以Winogradsky无氮培养基和PKO溶磷培养基从红豆草根瘤中分离菌株。按解磷-固氮-结瘤的顺序逐级筛选,结合培养观察和回接鉴定。最终获得3株溶磷根瘤菌RS-1,RS-8和RS-14。其中RS-1产生长素能力及回接根瘤固氮酶活性均较高,溶磷量6.98mg·L-1·d-1,适应pH4~12的环境并耐受11%的盐胁迫。表明该筛选方法简便有效,菌株应用前景好。

关键词: 红豆草, 根瘤菌, 固氮, 溶磷, 生长素

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