Acta Agrestia Sinica ›› 2017, Vol. 25 ›› Issue (6): 1265-1273.DOI: 10.11733/j.issn.1007-0435.2017.06.016

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Effects of Salicylic Acid on Physiological Characteristics and Growth of Alfalfa Seedling under NaCl Stress

LU Ya-ling, WANG Ling-jing, WANG Ning, SHAO Chun-lai, LI Zhi-hua   

  1. College of Prataculture Science, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China
  • Received:2016-11-24 Revised:2018-01-08 Online:2017-12-15 Published:2018-03-30

外源水杨酸对NaCl胁迫下紫花苜蓿幼苗生长和生理特性的影响

逯亚玲, 王灵婧, 王宁, 邵春来, 李志华   

  1. 南京农业大学草业学院, 江苏 南京 210095
  • 通讯作者: 李志华,E-mail:lizhihua@njau.edu.cn
  • 作者简介:逯亚玲(1994-),女,青海海东人,硕士,E-mail:2015120003@njau.edu.cn
  • 基金资助:

    国家大学生创新创业训练计划项目(201610307038)资助

Abstract:

In order to ascertain the effects of salicylic acid on physiological characteristics and growth of Alfalfa (Medicago sativa) seedling under NaCl stress, a pot experiment of ‘Weston’ alfalfa cultivars was established. In the study, the effects of different concentrations of exogenous salicylic acid (control 0, 0.5, 1.5, and 2.5 mmol·L) with foliar spray on alfalfa seedling growth and physiological under the condition of different NaCl stress (0, 0.3%, 0.6%, 0.9%, 1.2%) were studied. The results indicated that as the aggravation of the salt stress alfalfa seedling growth was significantly inhibited, while adding exogenous 0.5 and 1.5 mmol·L-1 salicylic acid significantly improved plant height, stem diameter, number of primary branches, and leaf relative water content under 0.6%, 0.9% and 1.2% salt stress. Moreover salicylic acid applications markedly increased the activities of protected enzymes such as superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), and decreased leaf relative conductivity and the content of malondialdehyde (MDA). Thus it can be seen that treatments with an appropriate concentration of salicylic acid can improve alfalfa growth and enhance its salt tolerance.

Key words: Alfalfa, Salt stress, Salicylic acid

摘要:

为了探明水杨酸对NaCl胁迫下紫花苜蓿(Medicago sativa)幼苗生长和生理特性的影响,以“威斯顿”紫花苜蓿品种为材料,采用盆栽试验方法,研究叶面喷施不同浓度水杨酸(0,0.5,1.5,2.5 mmol·L-1)对不同NaCl处理(0、0.3%、0.6%、0.9%、1.2%)下紫花苜蓿苗期生长及抗性生理的影响。结果表明,随着NaCl浓度的增加,紫花苜蓿幼苗生长受到显著抑制,在0.6%、0.9%和1.2%盐浓度胁迫下,添加0.5、1.5 mmol·L-1外源水杨酸后,紫花苜蓿的株高、茎粗、一级分枝数、叶片相对含水量均显著提高(P<0.05);同时,叶片中的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性显著升高;相对电导率、丙二醛(MDA)含量显著降低(P<0.05)。可见,适宜浓度水杨酸可以改善不同浓度NaCl胁迫下紫花苜蓿的生长状况,提高其耐盐能力。

关键词: 紫花苜蓿, 盐胁迫, 水杨酸

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