Acta Agrestia Sinica ›› 2019, Vol. 27 ›› Issue (5): 1163-1171.DOI: 10.11733/j.issn.1007-0435.2019.05.007

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Screening and Evaluation of Hybrid Combinations of the Bar Transgenic Alfalfa

ZHOU Le1,2, WANG Ying-zhe2, XU Bo1, Tan Jing1, XU An-kai1,2   

  1. 1. College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin Province 130118, China;
    2. Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences, Changchun, Jilin Province 130118, China
  • Received:2018-05-28 Revised:2019-07-23 Online:2019-10-15 Published:2019-11-09

转Bar基因紫花苜蓿杂交组合的筛选与评价

周仂1,2, 王英哲2, 徐博1, 谭晶1, 徐安凯1,2   

  1. 1. 吉林农业大学动物科学技术学院, 吉林 长春 130118;
    2. 吉林省农业科学院, 吉林 长春 130118
  • 通讯作者: 徐安凯
  • 作者简介:周仂(1994-),男,甘肃省张掖人,硕士研究生,研究方向为牧草种质资源与遗传育种,E-mail:zhoule228@126.com
  • 基金资助:
    吉林省科技厅优秀青年基金项目(20190103103JH);吉林省农业科学院结余经费(统筹部分)资助项目(y81990501)共同资助

Abstract: To explore and validate the efficient hybrid breeding technology of herbicide-tolerant transgenic alfalfa,one control group was conducted using the alfalfa ‘GN-1’ as the male and female parents;11 hybrid combinations were conducted using 11 glyphosate-resistant herbicide transgenic alfalfa as the male parent and the male sterile line MS-GN-1A as the female parent. The resistance of progeny plants was tested by test paper and PCR,and the yield and quality traits were evaluated and screened. We found that the progenies of 7 hybrid combinations showed excellent performance. Our research used male sterile lines as hybrid seeds to make the target traits of herbicide-tolerant transgenic alfalfa inherit efficiently and stability. Additionally,we have screened out excellent hybrid offspring,which provides a certain theoretical basis for seed breeding.

Key words: Transgenic alfalfa, Male sterility, Glufosinate resistant

摘要: 为探究抗除草剂转基因紫花苜蓿(Medicago sativa)的高效杂交种选育技术。本试验以‘公农1号’作为父母本配置了1个对照组合,以转Bar基因的抗草铵膦除草剂转基因紫花苜蓿作为父本,以雄性不育系MS-GN-1A作为母本,配置了11个杂交组合。对后代植株的抗性进行Bar试纸条、PCR检测,对产量和品质性状进行评价和筛选,结果表明,试验中有7个杂交组合的后代表现优异。此试验以紫花苜蓿雄性不育系作为母本配置杂交种,使抗除草剂转基因紫花苜蓿的目标性状高效且稳定遗传,同时在此试验基础上筛选出了优良杂交后代,为良种繁育提供了一定的理论基础。

关键词: 转基因紫花苜蓿, 雄性不育, 抗草铵膦

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