Acta Agrestia Sinica ›› 2021, Vol. 29 ›› Issue (7): 1589-1593.DOI: 10.11733/j.issn.1007-0435.2021.07.028

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Karyotype Analysis of Pennisetum centrasiaticum in Shigatse,Tibet

ZHANG Li-tian1,4,5, LIU De-mei2, LIU Bo2,3, YANG Shi-hai4, WANG Yan-long1, WANG Xiao-li1, MA Yu-shou1   

  1. 1. Academy of Animal Science and Veterinary Medicine, Qinghai university, Xining, Qinghai Province 810016, China;
    2. Northwest Plateau Institute of Biology, Chinese Academy of Science/Key Laboratory of Adaptation and Evolution, Chinese Academy of Science/Key Laboratory of Crop Molecular Breeding in Qinghai Province, Xining, Qinghai Province 810008, China;
    3. University of Chinese Academy of Science, 100049, Beijing, China;
    4. Tibet Yunwang Industrial Co., Ltd., Shigatse, Tibet 857000, China;
    5. Shigatse Baiyacheng Agricultural Products Processing Co., Ltd., Shigatse, Tibet 857000, China
  • Received:2021-03-26 Revised:2021-04-22 Online:2021-07-15 Published:2021-07-31

西藏自治区日喀则市白草核型分析

张力天1,4,5, 刘德梅2, 刘博2,3, 杨时海4, 王彦龙1, 王晓丽1, 马玉寿1   

  1. 1. 青海大学畜牧兽医科学院, 青海 西宁 810016;
    2. 中国科学院西北高原生物研究所/中国科学院高原生物适应与进化重点实验室/青海省作物分子育种重点实验室, 青海 西宁 810008;
    3. 中国科学院大学, 北京 100049;
    4. 西藏云旺实业有限公司, 西藏 日喀则 857000;
    5. 日喀则市百亚成农牧产品加工有限公司, 西藏 日喀则 857000
  • 通讯作者: 杨时海,E-mail:shihaiyang@163.com;马玉寿,E-mail:mayushou@sina.com
  • 作者简介:张力天(1987-),男,四川宜宾人,博士研究生,主要从事牧草育种与栽培研究,E-mail:litzhang@foxmail.com
  • 基金资助:
    日喀则市种质资源利用与创新综合研究基地;2019-县域创新试点县(祁连县)资助

Abstract: Pennisetum centrasiaticum is widely distributed all over the world, and it is kind of forage grazed by cattle and sheep. In this study,a conventional squash technique was used to analyze the karyotype of P. centrasiaticum collected from shigatse. Results showed that the sum of chromosome lengths was 45.87 μm,the average arm ratio of chromosome was 1.23,and the index of karyotypic asymmetry was 54.83. All chromosomes with the arm ratio ranged from 1.08 to 1.70,one pair of chromosomes was sm,and the remaining chromosomes were m. There was no satellite on the chromosome of P. centrasiaticum. The basic chromosome number was 9,and P. centrasiaticum was a tetraploid plant with karyotype formula 2n=4x=36=34 m+2 sm,and belonged to Stebbins’ 1A type. It meant P. centrasiaticum was a primitive species in evolution.

Key words: Pennisetum centrasiaticum Tzvel., Chromosome, Karyotype, Tibet

摘要: 白草(Pennisetum centrasiaticum)广泛分布于世界各地,被牛羊所采食。本试验采用常规根尖压片法对采自西藏日喀则市的白草进行染色体数目统计及核型分析。结果表明:白草染色体总长度为45.87 μm,平均臂比为1.23,核型不对称指数为54.83;所有染色体的臂比在1.08至1.70范围内,其中近中部着丝粒染色体(sm)为1对,其余17对均为中部着丝粒染色体(m),所有染色体未观测到随体出现;白草染色体基数为9,核型为2n=4x=36=34 m+2 sm,属于四倍体植物,核型类型为1A,是进化较原始的物种。

关键词: 白草, 染色体, 核型, 西藏

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