Isolation,Identification and Pathogenicity Analysis of Helminthosporioid Fungi from Silage Maize

doi:10.11733/j.issn.1007-0435.2023.01.005

Acta Agrestia Sinica ›› 2023, Vol. 31 ›› Issue (1): 40-49.DOI: 10.11733/j.issn.1007-0435.2023.01.005

Isolation,Identification and Pathogenicity Analysis of Helminthosporioid Fungi from Silage Maize

QI He-xing¹, WANG Hai-chun², LU Guang-xin¹, LI Zong-ren¹, TIAN Feng¹, HE Chen-bang³, XU Cheng-ti⁴, ZOU Hai-tao¹, LEI Sheng-yan¹, LI Hong-fang¹, TANG Hai-peng¹

1. College of Agriculture and Animal Husbandry, Qinghai University, Xining, Qinghai Province 810016, China;
2. Head Station of Grassland of Qinghai Province, Xining, Qinghai Province 810001, China;
3. Academy of Agriculture and Forestry Sciences, Qinghai University, Xining, Qinghai Province 810016, China;
4. Institute of Animal Husbandry and Veterinary Sciences，Qinghai University, Xining, Qinghai Province 810016, China

Received:2022-10-13 Revised:2022-11-13 Online:2023-01-15 Published:2023-02-07

青贮玉米蠕形菌的分离鉴定及其致病性分析

祁鹤兴¹, 王海春², 芦光新¹, 李宗仁¹, 田丰¹, 贺晨邦³, 徐成体⁴, 邹海涛¹, 雷生妍¹, 李红芳¹, 唐海鹏¹

1. 青海大学农牧学院, 青海西宁 810016;
2. 青海省草原总站, 青海西宁 810001;
3. 青海大学农林科学院, 青海西宁 810016;
4. 青海大学畜牧兽医科学院, 青海西宁 810016

通讯作者: 芦光新, E-mail:lugx74@qq.com
作者简介:祁鹤兴(1990-)，女，青海湟中人，博士，副教授，主要从事植物病原真菌及病害生物防治研究，E-mail:qhx390495559@126.com
基金资助:
青海省饲草产业科技创新平台和小麦平台玉米功能室；2022年中央财政林业和草原生态保护恢复资金项目-青海省牧草病害种类及分布调查；青海大学2020年科研启动金(4139040111)资助

Abstract

Abstract: In this study leaf spots of silage maize were suspected to be caused by Helminthosporioid fungi,and samples were collected,pathogens were isolated. We then analyzed the morphological characteristics,phylogeny of ITS-rDNA sequence and GADPH gene sequence of pathogens,and tested the pathogenicity for understanding the species of Helminthosporioid fungi pathogens from silage maize. All the 142 isolates belonged to 3 genera and 6 species.Bipolaris sorokiniana and B. zeicola were the dominant pathogens,with 77 and 48 isolates,respectively. And 9 isolates were Exserohilum turcicum. The number of Curvularia verruculosa,C. lunata and C. spicifera pathogens were 2,3 and 3,respectively. Pathogenicity tests showed that B. sorokiniana and B. zeicola could infect 8 cultivars of silage maize,and the pathogenicity on 8 cultivars of another 4 species was different. Here,we preliminary clarified the species and pathogenicity of Helminthosporioid fungi causing leaf spot disease on silage maize,and lay a foundation for disease diagnosis and comprehensive prevention.

Key words: Silage maize, Helminthosporioid, Leaf spot, Phylogeny, Pathogenicity

摘要： 本研究为初步明确引起青贮玉米(Zea Mays L.)叶斑病的蠕形菌种类，采集疑似病斑叶片样本，对病原菌进行分离纯化，形态特征观察，ITS-rDNA序列和GADPH基因序列系统发育分析及致病性测定。形态学和分子生物学鉴定结果表明分离得到的142株病原菌为3属6种真菌，其中麦根腐平脐蠕孢(Bipolaris sorokiniana)和玉米生平脐蠕孢(B. zeicola)为优势病原菌，分别为77株和48株；其次为大斑病菌(Exserohilum turcicum)9株，疣状弯孢(Curvularia verruculosa)2株，新月弯孢(C. lunata)3株，穗状弯孢(C. spicifera)3株。致病性测定发现麦根腐平脐蠕孢和玉米生平脐蠕孢对青海省8个主栽青贮玉米品种均有致病性，其余4种病原菌的致病性各有差异。本研究初步明确引起青贮玉米叶斑病的蠕形菌种类及其致病性，为后续开展病害诊断及其综合防控奠定了基础。

关键词: 青贮玉米, 蠕形菌, 叶斑病, 系统发育分析, 致病性

CLC Number:

S816.5+3

QI He-xing, WANG Hai-chun, LU Guang-xin, LI Zong-ren, TIAN Feng, HE Chen-bang, XU Cheng-ti, ZOU Hai-tao, LEI Sheng-yan, LI Hong-fang, TANG Hai-peng. Isolation,Identification and Pathogenicity Analysis of Helminthosporioid Fungi from Silage Maize[J]. Acta Agrestia Sinica, 2023, 31(1): 40-49.

祁鹤兴, 王海春, 芦光新, 李宗仁, 田丰, 贺晨邦, 徐成体, 邹海涛, 雷生妍, 李红芳, 唐海鹏. 青贮玉米蠕形菌的分离鉴定及其致病性分析[J]. 草地学报, 2023, 31(1): 40-49.

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Isolation,Identification and Pathogenicity Analysis of Helminthosporioid Fungi from Silage Maize

青贮玉米蠕形菌的分离鉴定及其致病性分析

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