Identification and Analysis of CNGC Gene Family Members in Alfalfa

doi:10.11733/j.issn.1007-0435.2024.02.028

Acta Agrestia Sinica ›› 2024, Vol. 32 ›› Issue (2): 588-598.DOI: 10.11733/j.issn.1007-0435.2024.02.028

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Identification and Analysis of CNGC Gene Family Members in Alfalfa

LI Xiao-hong¹, WANG Xiao-tong¹, MA Xu-xia¹, CAI Wen-qi¹, FENG Xue-li¹, MA Meng-fan¹, LI Shu-xia^1,2,3

1. College of Forestry and Prataculture, Ningxia University, Yinchuan, Ningxia 750021, China;
2. Ningxia Grassland and Animal Husbandry Engineering Technology Research Center, Yinchuan, Ningxia 750021, China;
3. Key Laboratory for Model Innovation in Forage Production Efficiency, Ministry of Agriculture and Rural Affairs, Yinchuan, Ningxia 750021, China

Received:2023-09-26 Revised:2023-11-16 Online:2024-02-15 Published:2024-03-06

紫花苜蓿CNGC基因家族成员鉴定及分析

李小红¹, 王晓彤¹, 麻旭霞¹, 蔡文祺¹, 冯学丽¹, 马梦凡¹, 李淑霞^1,2,3

1. 宁夏大学林业与草业学院, 宁夏银川 750021;
2. 宁夏草牧业工程技术研究中心, 宁夏银川 750021;
3. 农业农村部饲草高效生产模式创新重点实验室, 宁夏银川 750021

通讯作者: 李淑霞,E-mail:lishuxia620@163.com
作者简介:李小红(1996-),男,回族,宁夏固原人,硕士研究生,主要从事牧草抗逆育种研究,E-mail:lxh0895@163.com
基金资助:
国家自然科学基金项目（32101426）；宁夏自然科学基金项目（2023AAC05019）资助

Abstract

Abstract: The cyclic nucleotide-gated channels (CNGCs) gene family functions as one of the non-selective cation channel gene families and plays important roles in physiological processes such as plant signal transduction,growth and development,and environmental stress. In this study,we analyzed the evolutionary relationship,gene structure,conserved motifs,cis-acting elements,chromosome localization,collinearity,and gene expression patterns of the CNGC family members in alfalfa using bioinformatic tools and transcriptomic data. The results showed that there were 16 members of MsCNGC genes family in alfalfa,which were unevenly distributed on chromosomes,with segmental repeation,and most of proteins were located in the plasma membrane. The phylogenetic tree divided MsCNGCs into four subfamilies. Gene structure and conserved motif analysis showed that cNMP/CNBD and ITP functional domains were present. Cis-acting element analysis showed that MsCNGC genes contained many elements that responded to abiotic or biological stresses and hormones. There was interaction between MsCNGC4 and MsCNGC7.2 proteins. MsCNGC genes had tissue specificity. MsCNGC genes had significant response to drought,salt and hormone stress,which provided a theoretical basis for further research on the potential function of MsCNGC genes in the regulation of abiotic stress.

Key words: Alfalfa, CNGC genes family, Genome-wide, Systematic evolution, Gene structure, Abiotic stress

摘要： 环核苷酸门控通道（CNGC）基因家族作为非选择性阳离子通道基因家族之一，在植物信号转导、生长发育和环境胁迫等生理过程中发挥着重要作用。本研究利用生物信息学手段和转录组数据对紫花苜蓿CNGC家族成员的进化关系、基因结构、保守基序、顺式作用元件、染色体定位、共线性关系以及基因表达进行分析。结果表明，紫花苜蓿MsCNGC基因家族成员有16个，在染色体上不均匀分布，存在片段重复，大多数蛋白定位于细胞质膜。系统发育树将MsCNGCs为4个亚家族。基因结构和保守基序分析表明，都含有cNMP/CNBD和ITP功能域。顺式作用元件分析表明，MsCNGCs基因含有许多与非生物或生物胁迫和激素响应元件。MsCNGC4和MsCNGC7.2蛋白之间存在互作。MsCNGC基因具有组织表达特异性。MsCNGC基因对干旱、盐和激素等非生物胁迫均有显著的响应，为进一步研究MsCNGC基因在调控非生物胁迫过程中的潜在功能提供了理论依据。

关键词: 紫花苜蓿, CNGC基因家族, 全基因组, 系统进化, 基因结构, 非生物胁迫

CLC Number:

S512.6

LI Xiao-hong, WANG Xiao-tong, MA Xu-xia, CAI Wen-qi, FENG Xue-li, MA Meng-fan, LI Shu-xia. Identification and Analysis of CNGC Gene Family Members in Alfalfa[J]. Acta Agrestia Sinica, 2024, 32(2): 588-598.

李小红, 王晓彤, 麻旭霞, 蔡文祺, 冯学丽, 马梦凡, 李淑霞. 紫花苜蓿CNGC基因家族成员鉴定及分析[J]. 草地学报, 2024, 32(2): 588-598.

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Identification and Analysis of CNGC Gene Family Members in Alfalfa

紫花苜蓿CNGC基因家族成员鉴定及分析

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