Cloning,Subcellular Localization and Expression Analysis of MtBOP from Medicago truncatula

doi:10.11733/j.issn.1007-0435.2024.10.002

Acta Agrestia Sinica ›› 2024, Vol. 32 ›› Issue (10): 3026-3033.DOI: 10.11733/j.issn.1007-0435.2024.10.002

Cloning,Subcellular Localization and Expression Analysis of MtBOP from Medicago truncatula

LI Yan-ru¹, DONG Di², CHAO Yue-hui¹

1. College of Grassland Industry and Grassland, Beijing Forestry University, Beijing 100083, China;
2. Institute of Ecological Conservation and Restoration, Chinese Academy of Forestry, Beijing 100091, China

Received:2024-05-17 Revised:2024-07-10 Published:2024-11-04

蒺藜苜蓿MtBOP的克隆、亚细胞定位及表达分析

李艳茹¹, 董笛², 晁跃辉¹

1. 北京林业大学/草业与草原学院, 北京 100083;
2. 中国林业科学研究院生态保护与修复研究所, 北京 100091

通讯作者: 晁跃辉,E-mail:chaoyuehui@bjfu.edu.cn
作者简介:李艳茹(1997-)，女，汉族，山东菏泽人，硕士研究生，主要从事草类植物育种研究，E-mail:liyanru03@163.com
基金资助:
内蒙古草业技术创新中心有限公司项目(CCPTZX2023B04)资助

Abstract

Abstract: BLADE-ON-PETIOLE (BOP) gene is involved in regulating important plant developmental processes such as leaf and flower morphogenesis and shedding. In this study, Medicago truncatula R108 was used as a material to explore the characteristics of MtBOP through bioinformatics analysis,subcellular localization and expression pattern analysis. The complete coding region of MtBOP gene is 1452 bp,encoding 483 amino acids. According to phylogenetic tree results,it is closely related to Pisum sativum and Trifolium pratense. It has no signal peptide according to signal peptide prediction analysis. Subcellular localization results showed that MtBOP was located in the nucleus and cytoplasm. The analysis of expression pattern showed that the expression level of this gene was up-regulated under the regulation of abscisic acid,cytokinin,salicylic acid and gibberellin,while the expression of auxin and methyl jasmonate was down-regulated. The yeast self-activation test showed that MtBOP had no self-activation activity. This study provides scientific basis for further study of MtBOP gene of Medicago truncatula,and BOP can be used for subsequent related protein experiments.

Key words: Medicago truncatula, MtBOP, Subcellular localization, Expression analysis, Transcription from activation

摘要： 叶柄上叶基因(BLADE-ON-PETIOLE,BOP)参与调控植物生长发育中如叶片和花朵的形态形成和脱落等重要发育过程。本研究将蒺藜苜蓿R108作为材料，通过生物信息学分析、亚细胞定位及表达模式分析等实验，探究MtBOP基因的特性。MtBOP基因完整编码区为1452 bp，编码483个氨基酸。根据系统发育进化树结果可知其与豌豆(Pisum sativum)和红三叶草(Trifolium pratense)亲缘关系最近。通过信号肽预测分析，其不具有信号肽。亚细胞定位结果显示MtBOP定位于细胞核和细胞质。表达模式分析表明，该基因的表达量在脱落酸、细胞分裂素、水杨酸、赤霉素调节下有上调趋势，而在生长素，茉莉酸甲酯调节下出现下调趋势。酵母自激活检测显示MtBOP不具有自激活活性。本研究为进一步探究蒺藜苜蓿的MtBOP基因提供了科学依据，而且BOP可用于后续相关基因功能实验。

关键词: 蒺藜苜蓿, MtBOP, 亚细胞定位, 表达分析, 转录自激活

CLC Number:

S541

LI Yan-ru, DONG Di, CHAO Yue-hui. Cloning,Subcellular Localization and Expression Analysis of MtBOP from Medicago truncatula[J]. Acta Agrestia Sinica, 2024, 32(10): 3026-3033.

李艳茹, 董笛, 晁跃辉. 蒺藜苜蓿MtBOP的克隆、亚细胞定位及表达分析[J]. 草地学报, 2024, 32(10): 3026-3033.

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Cloning,Subcellular Localization and Expression Analysis of MtBOP from Medicago truncatula

蒺藜苜蓿MtBOP的克隆、亚细胞定位及表达分析

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