Chicory TCP Gene Family Identification and Analysis

doi:10.11733/j.issn.1007-0435.2024.08.010

Acta Agrestia Sinica ›› 2024, Vol. 32 ›› Issue (8): 2428-2439.DOI: 10.11733/j.issn.1007-0435.2024.08.010

Chicory TCP Gene Family Identification and Analysis

DU Yang^1,2, ZHANG Zhi-wei³, LU Shuang¹, LIU Xue², LI Da-yong², XU Zhao-yang², WANG Chen-chen², XU Li-xin¹, ZHANG Bin²

1. College of Prataculture and Grassland, Beijing Forestry University, Beijing 100083, China;
2. Institute of Vegetables, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100083, China;
3. CCTEG Ecological Environment Technology Co., Ltd, Beijing 100020, China

Received:2023-11-02 Revised:2023-12-29 Published:2024-09-07

菊苣TCP基因家族鉴定与分析

杜洋^1,2, 张智韦³, 陆爽¹, 刘雪², 李大勇², 徐朝阳², 王晨晨², 许立新¹, 张彬²

1. 北京林业大学草业与草原学院, 北京 100083;
2. 北京市农林科学院蔬菜所, 北京 100083;
3. 中煤科工生态环境科技股份有限公司, 北京 100020

通讯作者: 许立新,E-mail:liberisa@163.com;张彬,E-mail:zhangbin@nercv.org;
作者简介:杜洋(1998-),女,汉族,河北承德人,硕士研究生,主要从事草业科学和分子学的研究,E-mail:2209437886@qq.com;张智韦(1995-),男,汉族,内蒙古鄂尔多斯人,硕士研究生,主要从事草业科学和分子学的研究,E-mail:1392303875@qq.com
基金资助:
北京市农林科学院蔬菜研究所改革与发展项目(KYCX202304);北京市农林科学院科技创新项目(KJCX20230123)资助

Abstract

Abstract: The TCP gene family is involved in the regulation of a variety of physiological processes in plants,including plant leaf development,flower development,and branch formation. Chicory(Cichorium intybus L.) is an excellent forage,but no studies of its TCP gene family have been reported. Based on the whole genome data of chicory,the amino acid sequences of the Arabidopsis thaliana and Lactuca sativa L. TCP gene families were used to identify and screen the members of the chicory TCP gene family,and finally 39 chicory TCP gene family members were obtained. Then,bioinformatics methods were used to analyze these members and found that 39 members were unevenly distributed on 9 chromosomes. The number of amino acids is 113~498,and the molecular weight is 12 425.84~56 266.57 Da. All members contain conserved TCP domains,namely basic-helix-loop-helix(bHLH) domains,and are divided into 3 subfamilies. The analysis of cis-acting elements showed that members of the chicory TCP family had a variety of hormones,abiotic stress and growth and development response elements. The expression analysis of CIN class TCP genes in chicory tissues and under high temperature stress showed that these members may be involved in the regulation of leaf development and response to high temperature stress in chicory. This study can provide a theoretical basis for improving the quality of chicory forage.

Key words: TCP gene family, Cichorium intybus L., Expression analysis, Family analysis

摘要： TCP转录因子参与调控植物多种生理过程，包括植物叶片发育、花发育、分枝的形成等方面。菊苣(Cichorium intybus L.)是一种优良牧草，但目前还未有关于其TCP基因家族研究的报道。本研究基于菊苣全基因组数据，利用拟南芥(Arabidopsis thaliana)、生菜(Lactuca sativa L.) TCP基因家族的氨基酸序列，对菊苣TCP基因家族成员进行鉴定筛选，最终得到39个菊苣TCP基因家族成员。接着利用生物信息学方法对这些成员进行分析发现，39个成员不均匀分布在9条染色体上；氨基酸数目为113～498，分子量为12 425.84～56 266.57Da；所有成员分为3个亚家族；均含有保守的basic-helix-loop-helix(bHLH)结构域；顺式作用元件分析发现，菊苣TCP家族成员具有多种激素、非生物胁迫和生长发育响应元件；对CIN类TCP基因在菊苣各组织和高温胁迫下进行表达分析发现，这些成员可能参与菊苣的叶片发育调控且对高温胁迫响应。本研究可以为改良菊苣牧草品质提供理论依据。

关键词: TCP基因家族, 菊苣, 表达分析, 家族分析

CLC Number:

Q949.783.5

DU Yang, ZHANG Zhi-wei, LU Shuang, LIU Xue, LI Da-yong, XU Zhao-yang, WANG Chen-chen, XU Li-xin, ZHANG Bin. Chicory TCP Gene Family Identification and Analysis[J]. Acta Agrestia Sinica, 2024, 32(8): 2428-2439.

杜洋, 张智韦, 陆爽, 刘雪, 李大勇, 徐朝阳, 王晨晨, 许立新, 张彬. 菊苣TCP基因家族鉴定与分析[J]. 草地学报, 2024, 32(8): 2428-2439.

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Chicory TCP Gene Family Identification and Analysis

菊苣TCP基因家族鉴定与分析

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