Identification and Expression Analysis of the GPAT Gene Family in Medicago sativa under Saline Alkali Stress

doi:10.11733/j.issn.1007-0435.2023.09.005

Acta Agrestia Sinica ›› 2023, Vol. 31 ›› Issue (9): 2608-2620.DOI: 10.11733/j.issn.1007-0435.2023.09.005

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Identification and Expression Analysis of the GPAT Gene Family in Medicago sativa under Saline Alkali Stress

TANG Fang, MEI Ting, GAO Jia-he, WANG Jia-ni, SHI Feng-ling, GAO Cui-ping

College of Grassland, Resource and Environment, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010010, China

Received:2023-04-26 Revised:2023-07-10 Online:2023-09-15 Published:2023-10-07

紫花苜蓿GPAT基因家族鉴定及在盐碱胁迫下的表达模式分析

唐芳, 梅亭, 高佳荷, 王佳妮, 石凤翎, 高翠萍

内蒙古农业大学草原与资源环境学院, 内蒙古呼和浩特 010010

通讯作者: 唐芳,E-mail:fta223@imau.edu.cn
作者简介:唐芳(1983-),女,汉族,贵州息烽人,博士,副教授,主要从事牧草栽培育种和植物-微生物互作研究,E-mail:fta223@imau.edu.cn
基金资助:
高层次留学人才回国工作资助项目，内蒙古自治区科技重大专项(2021ZD0031)；内蒙古农业大学高层次人才引进科研启动项目(NDGCC2016-18)资助

Abstract

Abstract: Glycerol-3-phosphate acyltransferase (GPAT) is the rate-limiting enzyme of glycerol biosynthesis,participates in the biosynthetic pathway of various lipids,and plays important roles in plant growth and stress resistance. To understand the role of GPAT genes in the response of alfalfa (Medicago sativa) to abiotic stress,a total of 73 MsGPATs genes in alfalfa were identified using bioinformatics methods in this study. Studies showed that the MsGPATs gene were unevenly distributed on chromosomes,and most genes exhibited segmental duplication. Phylogenetic tree analysis was classified into three subgroups. Gene structure and protein conservative motif analysis showed that most of the MsGPATs gene family members contained 2~3 exons or 11~12 exons,and at least one conserved motif. Promoter cis-acting elements analysis showed that different MsGPATs genes contained different light,auxin and stress response elements. Gene expression profiling analysis showed that a total of 10,5 and 18 MsGPATs genes actively responded to high salt,high alkali and mixed saline-alkali stress,respectively,which could be used as candidate genes for further research on saline and alkali tolerance in alfalfa.

Key words: Medicago sativa, GPAT gene family, Saline alkali stress, Expression analysis

摘要： 甘油-3-磷酸酰基转移酶(Glycerol-3-phosphate acyltransferase,GPAT)是甘油生物合成的限速酶,参与多种脂类的生物合成途径,在植物生长发育和抗逆过程中具有重要作用。为了解GPAT基因在紫花苜蓿(Medicago sativa)应对非生物胁迫过程中的作用,本研究利用生物信息学方法共鉴定出73个紫花苜蓿MsGPATs基因。研究表明,MsGPAT基因在染色体上不均匀分布,大多数基因存在大片段复制现象。系统进化树分析将其分为3个亚组。基因结构和蛋白质保守基序分析表明MsGPAT基因家族成员多数含有2~3个外显子或11~12个外显子,至少含有1个保守基序。顺式作用元件分析表明不同MsGPATs基因含有不同的光、生长素和胁迫应答元件。基因表达谱分析显示,分别有10,5,18个MsGPATs基因积极响应高盐、高碱和混合盐碱胁迫,可作为紫花苜蓿耐盐碱研究的候选基因。

关键词: 紫花苜蓿, GPAT基因家族, 盐碱胁迫, 表达分析

CLC Number:

S541.9

TANG Fang, MEI Ting, GAO Jia-he, WANG Jia-ni, SHI Feng-ling, GAO Cui-ping. Identification and Expression Analysis of the GPAT Gene Family in Medicago sativa under Saline Alkali Stress[J]. Acta Agrestia Sinica, 2023, 31(9): 2608-2620.

唐芳, 梅亭, 高佳荷, 王佳妮, 石凤翎, 高翠萍. 紫花苜蓿GPAT基因家族鉴定及在盐碱胁迫下的表达模式分析[J]. 草地学报, 2023, 31(9): 2608-2620.

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References

[1] 刘俊羽,杨帆,毛爽,等. 植物脂质应答逆境胁迫生理功能的研究进展[J]. 生物工程学报,2021,37(8):2658-2667
[2] 姚楠. 植物脂质生物学进展[J]. 植物生理学报,2018,54 (12):1747
[3] WASCHBURGER E,KULCHESKI F R,VETO N M,et al. Genome-wide analysis of the Glycerol-3-Phosphate Acyltransferase (GPAT) gene family reveals the evolution and diversification of plant GPATs[J]. Genetics & Molecular Biology,2018,41(1 suppl 1):355-370
[4] 杨成兰,段瑞君,武雄雄,等. 蒺藜苜蓿GPAT基因家族的全基因组鉴定、序列变异和表达分析[J]. 草业科学,2021,38(10):1966-1974
[5] SINGER S D,CHEN G,MIETKIEWSKA E,et al. Arabidopsis GPAT9 contributes to synthesis of intracellular glycerolipids but not surface lipids[J]. Journal of Experimental Botany,2016,67(15):4627-4638
[6] KIM H U,HUANG A H. Plastid lysophosphatidyl acyltransferase is essential for embryo development in Arabidopsis[J]. Plant Physiology,2004,134(3):1206-1216
[7] 刘聪,肖旦望,施春霖,等. 植物GPATs基因研究进展[J]. 遗传,2013,35(12):1352-1359
[8] NISHIDA I,TASAKA Y,SHIRAISHI H,et al. The gene and the RNA for the precursor to the plastid-located glycerol-3-phosphate acyltransferase of Arabidopsis thaliana[J]. Plant Molecular Biology,1993,21(2):267-277
[9] 李红梅. 百合GPAT基因的定位及功能研究[D]. 沈阳:沈阳农业大学,2016:1
[10] 杨成兰,武雄雄,祁存英,等. 大麦GPAT基因家族在环境抗逆中的功能分析[C]//中国作物学会.第十九届中国作物学会学术年会论文摘要集. 武汉:华中农业大学,2020:205
[11] SAFDER I,SHAO G,SHENG Z,et al. Identification and analysis of the structure,expression and nucleotide polymorphism of the GPAT gene family in rice[J]. Plant Gene,2021(26):100290
[12] 吕思. 连翘属抗寒相关基因GPAT的克隆、表达及功能分析[D]. 沈阳:沈阳农业大学,2017:1
[13] JAYAWARDHANE K N,SINGER S D,WESELAKE R J,et al. Plant sn-glycerol-3-phosphate acyltransferases:biocatalysts involved in the biosynthesis of intracellular and extracellular lipids[J]. Lipids,2018,53(5):469-480
[14] ZHENG Z,XIA Q,DAUK M,et al. Arabidopsis AtGPAT1,a member of the membrane-bound glycerol-3-phosphate acyltransferase gene family,is essential for tapetum differentiation and male fertility[J]. The Plant Cell Online,2003,15(8):1872-1887
[15] CHEN X,SNYDER C L,TRUKSA M,et al. sn-Glycerol-3-phosphate acyltransferases in plants[J]. Plant Signaling & Behavior,2011,6(11):1695-1699
[16] LI X C,ZHU J,YANG J,et al. Glycerol-3-phosphate acyltransferase 6 (GPAT6) is important for tapetum development in Arabidopsis and plays multiple roles in plant fertility[J]. Molecular Plant,2012,5(1):131-142
[17] BEISSON F,LI Y,BONAVENTURE G,et al. The acyltransferase GPAT5 is required for the synthesis of suberin in seed coat and root of Arabidopsis[J]. Plant Cell,2007,19(1):351-368
[18] YANG W,SIMPSON J P,LI-BEISSON Y,et al. A land-plant-specific glycerol-3-phosphate acyltransferase family in Arabidopsis:Substrate specificity,sn-2 preference,and evolution[J]. Plant Physiology,2012,160(2):638-652
[19] 郝静芳. 拟南芥甘油-3-磷酸酰基转移酶的三个基因(GPAT6,7,9)在种子油脂合成及幼苗耐盐中的作用[D]. 南京:南京农业大学,2013:1
[20] SUI N,TIAN S,WANG W,et al. Overexpression of glycerol-3-phosphate acyltransferase from Suaeda salsa improves salt tolerance in Arabidopsis[J]. Frontiers in Plant Science,2017(8):1337
[21] 耿华珠. 中国苜蓿[M]. 北京:中国农业出版社,1995:1-2
[22] LI X,WEI Y,MOORE K J,et al. Association mapping of biomass yield and stem composition in a tetraploid alfalfa breeding population[J]. The Plant Genome,2011,4(1):24-35
[23] 李刚. 紫花苜蓿研究进展[J]. 安徽农学通报,2019,25(14):71-75
[24] 郑普山,郝保平,冯悦晨,等. 紫花苜蓿对盐碱地的改良效果[J]. 山西农业科学,2012,40(11):1204-1206
[25] 李雪,沙栢平,高雪芹,等. 不同紫花苜蓿种质材料萌发期耐盐性鉴定与综合评价[J]. 草地学报,2020,28(2):437-445
[26] 柴金平. 紫花苜蓿的价值和种植方法[J]. 养殖技术顾问,2019(3):44-45
[27] 马倩,闫启,张正社,等. 紫花苜蓿CCoAOMT基因家族的鉴定,进化及表达分析[J]. 草业学报,2021,30(11):144-156
[28] 齐晓,张正社,闵学阳,等. 紫花苜蓿bZIP基因家族的鉴定,进化及表达分析[J]. 草业科学,2017,34(8):1635-1648
[29] 魏娜,李艳鹏,马艺桐,等. 全基因组水平紫花苜蓿TCP基因家族的鉴定及其在干旱胁迫下表达模式分析[J]. 草业学报,2022,31(1):118-130
[30] 金小煜. 全基因组水平紫花苜蓿铜转运(COPT/Ctr)基因家族的鉴定及功能分析[D]. 兰州:兰州大学,2020:1
[31] 张学云,王瑜,辛宝宝,等. ST紫花苜蓿与'中苜一号'耐盐性研究[J]. 草地学报,2015,23(2):328-337
[32] CHEN H,ZENG Y,YANG Y,et al. Allele-aware chromosome-level genome assembly and efficient transgene-free genome editing for the autotetraploid cultivated alfalfa[J]. Nature Communications,2020,11(1):2494-2505
[33] CHAO J,LI Z,SUN Y,et al. MG2C:a user-friendly online tool for drawing genetic maps[J]. Mol Horticulture,2021,1(1):16-20
[34] CHEN C,CHEN H,ZHANG Y,et al. TBtools:An Integrative Toolkit Developed for Interactive Analyses of Big Biological Data[J]. Molecular Plant,2020,13(8):1194-1202
[35] KUMAR S,STECHER G,TAMURA K. MEGA7:Molecular evolutionary genetics analysis version 7.0 for bigger datasets[J]. Molecular Biology and Evolution,2015(33):1870-1874
[36] SUBRAMANIAN B,GANG S,LERCHER M J,et al. Evolview v3:a webserver for visualization,annotation,and management of phylogenetic trees[J]. Nuclc Acids Research,2019,47(1):270-275
[37] WANG Y,TANG H,DEBARRY J D,et al. MCScanX:a toolkit for detection and evolutionary analysis of gene synteny and collinearity[J]. Nucleic Acids Research,2012,40(7):49
[38] KRZYWINSKI M,SCHEIN J,BIROL I,et al. Circos:an information aesthetic for comparative genomics[J]. Genome Research,2009,19(9):1639-1645
[39] 杨紫贻. 紫花苜蓿耐盐评价体系的建立及耐盐机理研究[D]. 呼和浩特:内蒙古农业大学,2021:8-12
[40] 蔺吉祥,高战武,王颖,等. 盐碱胁迫对紫花苜蓿种子发芽的协同影响[J]. 草地学报,2014,22(2):312-318
[41] 王东明,贾媛,崔继哲. 盐胁迫对植物的影响及植物盐适应性研究进展[J]. 中国农学通报,2009(4):124-128
[42] ZHU T,WU S,ZHANG D,et al. Genome-wide analysis of maize GPAT gene family and cytological characterization and breeding application of ZmMs33/ZmGPAT6 gene[J]. Theoretical and Applied Genetics,2019,132(7):2137-2154
[43] CUI Y,MA J,LIU G,et al. Genome-wide identification,sequence variation,and expression of the glycerol-3-phosphate acyltransferase (gpat) gene family in gossypium[J]. Front Genet,2019(10):116
[44] 李晓川. 拟南芥甘油-3-磷酸乙酰转移酶6(GPAT6)对于绒毡层及育性的影响[D]. 上海:上海师范大学,2011:1
[45] SHOCKEY J,REGMI A,COTTON K,et al. Identification of arabidopsis gpat9 (at5 g60620) as an essential gene involved in triacylglycerol biosynthesis[J]. Plant Physiology,2015,170(1):163-179

Identification and Expression Analysis of the GPAT Gene Family in Medicago sativa under Saline Alkali Stress

紫花苜蓿GPAT基因家族鉴定及在盐碱胁迫下的表达模式分析

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