Cloning and Expression Analysis of the AcdMYB1 Gene from Agropyron critatum × A.desertorum ‘Hycrest-Mengnong’

doi:10.11733/j.issn.1007-0435.2023.08.009

Acta Agrestia Sinica ›› 2023, Vol. 31 ›› Issue (8): 2334-2342.DOI: 10.11733/j.issn.1007-0435.2023.08.009

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Cloning and Expression Analysis of the AcdMYB1 Gene from Agropyron critatum × A.desertorum ‘Hycrest-Mengnong’

HUANG Xin-tian¹, HAN Hui-jie¹, LI Yu-chen¹, LIU Ya-ling², ZHANG Ya-rong², ZHAO Yan¹

1. College of Grassland, Resources and Environmental Science, Inner Mongolia Agricultural University/Key Laboratory of Forage Cultivation, Processing and High Efficient Utilization of Ministry of Agriculture/Key Laboratory of Grassland Resources, Ministry of Education, Hohhot, Inner Mongolia 010018, China;
2. MenCao Ecology and Environment(Group) Co., Ltd, Hohhot, Inner Mongolia 010030, China

Received:2023-02-02 Revised:2023-04-26 Online:2023-08-15 Published:2023-09-01

蒙农杂种冰草AcdMYB1基因克隆及表达分析

黄馨田¹, 韩慧杰¹, 李宇琛¹, 刘亚玲², 张雅荣², 赵彦¹

1. 内蒙古农业大学草原与资源环境学院/农业部饲草栽培、加工与高效利用重点实验室/教育部草地资源重点实验室, 内蒙古呼和浩特 010018;
2. 蒙草生态环境(集团)股份有限公司, 内蒙古呼和浩特 010030

通讯作者: 赵彦,E-mail:zhaoyannmg@imau.edu.cn
作者简介:黄馨田(1998-),男,汉族,内蒙古乌海人,硕士研究生,主要从事牧草种质创新与遗传育种研究,E-mail:huangxintian1998@126.com
基金资助:
国家自然科学基金(32160326)；内蒙古自治区种业科技创新重大示范工程“揭榜挂帅”项目(2022JBGS0014);内蒙古自治区直属高校基本科研业务费种业振兴专项(BR22-11-10)资助

Abstract

Abstract: Agropyron cristatum×A. desertorum 'Hycrest-Mengnong' is a pasture variety cultivated by Inner Mongolia Agricultural University and it a tetraploid grass (2n=28) with high hay yield,good palatability and strong resistance to stress. It is a suitable grass species for establishing artificial grassland in arid areas of north China. A MYB transcription factor gene was cloned from A. cristatum×A.desertorum 'Hycrest-Mengnong' by homology-based cloning,named as AcdMYB1,the bioinformatics analysis and expression analysis were carried out on. The results showed that a cDNA sequence with a length of 1 161 bp and a maximum open reading frame of 969 bp encoding 322 amino acids,the AcdMYB1 sequence had a conserved domain of MYB transcription factor. AcdMYB1 had a genetic relationship with Aegilops tauschii,Triticum aestivum and Triticum dicoccoides by more than 90% sequence homology. The expression analysis results showed that the expression of AcdMYB1 gene was the highest in the spike,followed by leaves,roots and stems. Under a drought stress,the expression level of AcdMYB1 was significantly reduced. The protein was localized in the nucleus. The study provided a scientific basis for further research on the AcdMYB1 gene and the MYB transcription factor family in A. cristatum×A.desertorum ‘Hycrest-Mengnong' .

Key words: Agropyron critatum × A.desertorum 'Hycrest-Mengnong', Gene cloning, Expression analysis, MYB transcription factor

摘要： 蒙农杂种'冰草(Agropyron cristatum×A. desertorum 'Hycrest-Mengnong')具有干草产量高，适口性好，抗逆性强的特点，是中国北方干旱地区建立人工草地的适宜草种。本研究利用同源克隆方法从'蒙农杂种'冰草中克隆得到的1个MYB类转录因子基因(命名为AcdMYB1)，对其进行了生物信息学分析和表达分析。结果表明：该基因cDNA序列长度为1 161 bp，包含完整开放阅读框(ORF)为969 bp，编码322个氨基酸，具有MYB转录因子的保守结构域。AcdMYB1与小麦族的山羊草、普通小麦、野生二粒小麦的同类基因亲缘关系最近，序列一致性均在90%以上。组织表达中AcdMYB1基因在穗中的表达量最高，其次为叶、根，茎中表达量最低；在模拟自然干旱胁迫下，能够显著降低AcdMYB1的表达水平。烟草亚细胞定位显示，该蛋白定位于细胞核。本研究为进一步探索'蒙农杂种'冰草AcdMYB1基因及MYB转录因子家族提供了科学依据。

关键词: 蒙农杂种冰草, 基因克隆, 表达分析, MYB转录因子

CLC Number:

Q785
Q786

HUANG Xin-tian, HAN Hui-jie, LI Yu-chen, LIU Ya-ling, ZHANG Ya-rong, ZHAO Yan. Cloning and Expression Analysis of the AcdMYB1 Gene from Agropyron critatum × A.desertorum ‘Hycrest-Mengnong’[J]. Acta Agrestia Sinica, 2023, 31(8): 2334-2342.

黄馨田, 韩慧杰, 李宇琛, 刘亚玲, 张雅荣, 赵彦. 蒙农杂种冰草AcdMYB1基因克隆及表达分析[J]. 草地学报, 2023, 31(8): 2334-2342.

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Cloning and Expression Analysis of the AcdMYB1 Gene from Agropyron critatum × A.desertorum ‘Hycrest-Mengnong’

蒙农杂种冰草AcdMYB1基因克隆及表达分析

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