›› 2009, Vol. 17 ›› Issue (4): 435-439.DOI: 10.11733/j.issn.1007-0435.2009.04.005

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Analysis of Different Expression of Genes in Zoysia Matrella Exposed to High Temperature Treatment

HUANG Jin-wen, WU Wen-xiang, CHEN Dong-mei, ZHENG Hong-yan, LIN Wen-xiong   

  1. Institute of Agroecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • Received:2008-12-22 Revised:2009-04-24 Online:2009-08-15 Published:2009-08-15

沟叶结缕草高温胁迫的基因差异表达及其作用机制

黄锦文, 吴文祥, 陈冬梅, 郑红艳, 林文雄   

  1. 福建农林大学农业生态研究所, 福州, 350002
  • 通讯作者: 林文雄
  • 作者简介:黄锦文(1967- ),女,福建闽清人,副教授,博士,主要从事作物生理生态及分子生态学研究,E-mail:huangjw1126@sohu.com
  • 基金资助:
    福建省生态学重点学科资助(0608537)

Abstract: Some studies on the genes of disease-resistance,insect-resistance and salt-tolerant in turfgrass have been carried out,however few information is available in the heat-tolerant genes.In order to study molecular mechanisms of heat resistance of Zoysia matrella,an investigation,detecting the related gene expression profiles exposed to high temperature stress,was conducted using suppression subtractive hybridization(SSH).After the reverse northern hybridization screening,a total of 35 ESTs from SSH-cDNA library were sequenced and all of the sequences of inserted fragments were analyzed with the aid of bioinformatics.It obtained 26 different functionally known genes.These genes were sorted into four groups such as signal transduction,metabolism,stress resistance,gene expression regulation and protein synthesis.

Key words: Zoysia matrella, High temperature stress, Suppression subtractive hybridization(SSH), Gene expression

摘要: 为了从基因表达水平研究沟叶结缕草(Zoysia matrella)高温耐热性的分子机制,利用SSH方法构建了沟叶结缕草对高温(40℃)处理响应的正向差减文库.通过reverse northern杂交方法筛选文库,挑选出高温胁迫处理下35个差异表达基因,对这些基因进行测序,并将测序所得结果与基因组数据库(NCBI)进行比对,从而获得了26个基因功能已知的差异表达基因.这些已知基因分别参与信号转导、植物代谢、植物抗逆性反应、基因表达调控和蛋白质合成等过程.

关键词: 沟叶结缕草, 高温胁迫, 抑制消减杂交, 基因表达

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