Acta Agrestia Sinica ›› 2025, Vol. 33 ›› Issue (4): 1327-1334.DOI: 10.11733/j.issn.1007-0435.2025.04.034

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Screening and Promoting Effects of Plant Growth-Promoting Fungi from Rhizosphere of Tall fescue

RAN Shu-qi, ZHU Tian-qi, YU Yi-ze, LI Han-yu, LIU Tie-yuan, XU Yue-fei   

  1. 1. College of Grassland Agriculture, Northwest A&F University, Yangling, Shannxi Province 712100, China
  • Received:2024-08-12 Revised:2024-11-06 Online:2025-04-15 Published:2025-04-28

高羊茅根际耐盐促生真菌的分离鉴定及其促生效果评价

冉姝琪, 朱天奇, 余易泽, 李晗玉, 刘铁芫, 许岳飞   

  1. 1. 西北农林科技大学草业与草原学院, 陕西 杨凌 712100
  • 通讯作者: 刘铁芫,E-mail:ltyuan@nwafu.edu.cn;许岳飞,E-mail:xuyuefei@nwafu.edu.cn
  • 作者简介:冉姝琪(1999-),女,汉族,贵州贵阳人,硕士研究生,主要从事牧草、草坪草逆境生物学研究,E-mail:ranshuqi@nwsuaf.edu.cn;朱天奇(2000-),女,汉族,黑龙江绥化人,硕士研究生,主要从事草坪草逆境相关研究,E-mail:1839287738@163.com
  • 基金资助:
    国家自然科学基金(No. 32371784;No. 32071888)资助

Abstract: Soil salinization has emerged as a significant factor limiting the sustainable development of agriculture in my country. Plant growth-promoting fungi possess unique advantages in enhancing saline-alkali soils and fostering plant growth. This study utilized salt-tolerant tall fescue (Festuca arundinacea Schreb.) ‘Titanium 2LS’ as the experimental material. Several fungi were isolated from the rhizosphere soil of ‘Titanium 2LS’ under salt stress. The classification of the isolated strains was determined through morphological and molecular biological methods, leading to the selection of three key fungal strains and their growth-promoting effects were verified through experimental trials. The results indicate that the isolated fungi belong to the genera FusariumAspergillus, and Cladosporium, respectively. Inoculation tests demonstrated that the three strains exhibited significant growth-promoting effects on tall fescue, resulting in marked increases in plant height, leaf width, and root length, as well as improved photosynthetic efficiency and K+/Na+ ratios, thereby alleviating the toxicity associated with salt stress and enhancing the salt tolerance of the plants. This study provides a theoretical foundation for improving the salt tolerance of tall fescue and ameliorating saline-alkali soils.

Key words: Tall fescue, Salt stress, Plant growth-promoting fungi, Growth status, Physiology

摘要: 土壤盐渍化已成为制约我国农业可持续发展的一个重要因素。植物生长促生真菌在盐碱地改良和促进植物生长方面具有独特优势。本研究以耐盐高羊茅(Festuca arundinacea Schreb.)‘钛极-2LS’(‘Titanium 2LS’)为研究材料,从‘钛极-2LS’在盐胁迫下的根际土壤中分离得到若干真菌,通过形态学和分子生物学确定菌株的分类地位,并挑选出3种关键真菌,再通过试验评价筛选菌株的促生效果,发现分离出的真菌分别属于镰刀菌属(Fusarium),曲霉菌属(Aspergillus)和枝孢菌属(Cladosporium)。接种试验表明,这3株菌株均对高羊茅具有良好的促生效果,可显著增加植物株高,叶宽和根长,并提高其光合效率和K+/Na+比值来缓解盐胁迫的毒害,从而提高植物的耐盐性。本研究为提高高羊茅耐盐性和盐碱地改良提供理论依据。

关键词: 高羊茅, 盐胁迫, 促生真菌, 生长状况, 生理指标

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