Nitrogen and Phosphorus Uptake and Root Characteristics of Medicago sativa in Response to Soil Phosphorus and Molybdenum Addition

doi:10.11733/j.issn.1007-0435.2025.09.007

Acta Agrestia Sinica ›› 2025, Vol. 33 ›› Issue (9): 2820-2831.DOI: 10.11733/j.issn.1007-0435.2025.09.007

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Nitrogen and Phosphorus Uptake and Root Characteristics of Medicago sativa in Response to Soil Phosphorus and Molybdenum Addition

LIU Qian^1,2, CHEN Wei-wei^1,2, GAO Hui¹, WAN Wei-fan³, ZHAO Kun¹, HU Hai-na¹, LI Hai-gang¹

1. College of Resources and Environmental Sciences, Inner Mongolia Agricultural University, Inner Mongolia key of soil quality and Nutrient Resources, Hohhot, Inner Monglia 010018, China;
2. Key Laboratory of Agricultural Ecological Security and Green Development at Universities of Inner Mongolia Autonomous Region, Hohhot, Inner Mongolia 010018, China;
3. Inner Mongolia Academy of Science and Technology, Hohhot, Inner Mongolia 010010, China

Received:2024-10-24 Revised:2025-02-24 Online:2025-09-15 Published:2025-09-22

紫花苜蓿幼苗氮磷养分吸收和根系特征对土壤磷和钼添加的响应

刘茜^1,2, 陈薇薇^1,2, 高卉¹, 万伟帆³, 赵坤¹, 胡海娜¹, 李海港¹

1. 内蒙古农业大学资源与环境学院, 内蒙古自治区土壤质量与养分资源重点实验室, 内蒙古呼和浩特 010018;
2. 农业生态安全与绿色发展自治区高等实验室, 内蒙古呼和浩特 010018;
3. 内蒙古科学技术研究院, 内蒙古呼和浩特 010010

通讯作者: 李海港，E-mail：haigangli@imau.edu.cn
作者简介:刘茜（1996-），女，汉族，内蒙古鄂尔多斯人，博士研究生，主要从事牧草根际营养研究，E-mail：liuqianlq@126.com
基金资助:
国家自然科学基金项目（32360072）；内蒙古自治区重点科技发展计划（2021GG0076）；内蒙古自治区2023年研究生科研创新项目（B20231090Z）资助

Abstract

Abstract: Phosphorus （P） and molybdenum （Mo） are essential elements influencing nitrogen （N） fixation and nutrient acquisition in legumes. This study investigated the effects of different P and Mo levels on the growth and nutrient uptake of Medicago sativa through a pot experiment. The results showed that low P significantly restricted plant height and biomass， whereas Mo addition had no effect. P addition increased shoot biomass by an average of 168% compared to the no-P treatment. The interaction between P and Mo influenced root development， under P supply， elevated Mo supply reduced total root length and root surface area. P addition enhanced root organic acid exudation， increased plant P concentration and accumulation， promoted nodulation and P absorption rate， and reduced the N∶P. Meanwhile， shoot N concentration decreased due to a dilution effect with P addition， but total N accumulation increased. Mo addition had no significant impact on nutrient uptake or nodulation. This study demonstrates that under the given grassland soil conditions， P is the primary limiting factor for Medicago sativa growth， while soil Mo levels are sufficient to meet plant needs. These findings provide insights into the nutrient acquisition strategies of Medicago sativa and offer a theoretical basis for legume nutrient management.

Key words: Medicago sativa, Phosphorus, Molybdenum, Nutrients uptake, Root characteristics

摘要： 磷和钼是影响豆科结瘤固氮和养分获取的重要元素，明确其生长限制因素具有重要意义。本研究通过盆栽试验探究不同磷和钼水平对紫花苜蓿生长及养分吸收的影响，结果表明，低磷显著限制了紫花苜蓿的株高和生物量，而钼的添加无显著影响。磷添加使地上部生物量较无磷处理平均提高168%。磷和钼的协同作用影响紫花苜蓿的根系发育，供磷条件下，钼水平的增加降低了总根长和根表面积。磷添加促进了根系有机酸的分泌，提高了植株磷浓度及累积量，并促进结瘤和磷吸收率，同时降低氮磷比。而地上部氮浓度因稀释效应随磷添加降低但累积量增加。钼添加对养分吸收及结瘤无显著影响。研究表明，该草原土壤条件下，磷是紫花苜蓿生长的主要限制因子，而土壤钼已满足其需求。本研究揭示了紫花苜蓿的养分获取策略，并为豆科植物的养分管理提供科学依据。

关键词: 紫花苜蓿, 磷, 钼, 养分吸收, 根系特征

CLC Number:

S963.22⁺3.3

LIU Qian, CHEN Wei-wei, GAO Hui, WAN Wei-fan, ZHAO Kun, HU Hai-na, LI Hai-gang. Nitrogen and Phosphorus Uptake and Root Characteristics of Medicago sativa in Response to Soil Phosphorus and Molybdenum Addition[J]. Acta Agrestia Sinica, 2025, 33(9): 2820-2831.

刘茜, 陈薇薇, 高卉, 万伟帆, 赵坤, 胡海娜, 李海港. 紫花苜蓿幼苗氮磷养分吸收和根系特征对土壤磷和钼添加的响应[J]. 草地学报, 2025, 33(9): 2820-2831.

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Nitrogen and Phosphorus Uptake and Root Characteristics of Medicago sativa in Response to Soil Phosphorus and Molybdenum Addition

紫花苜蓿幼苗氮磷养分吸收和根系特征对土壤磷和钼添加的响应

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