Identification of Buchloe Dactyloides BdGH3 Gene Family and Analysis of Expression Pattern under Drought Stress

doi:10.11733/j.issn.1007-0435.2025.11.004

Acta Agrestia Sinica ›› 2025, Vol. 33 ›› Issue (11): 3515-3525.DOI: 10.11733/j.issn.1007-0435.2025.11.004

Identification of Buchloe Dactyloides BdGH3 Gene Family and Analysis of Expression Pattern under Drought Stress

WANG Xue-ping¹, WANG Si-ning², QI Hao-le³, ZHANG Ran², LI Xiao-xia², MA Hui-ling¹

1. College of Pratacultural Science, Gansu Agricultural University, Key Laboratory Pratacultural Ecosystem, Ministry of Education, Pratacultural Engineering Laboratory of Gansu Province, Sino-US Center for Grazingland Ecosystem Sustainability, Lanzhou, Gansu Province 730070, China;
2. Institute of Ecological Conservation and Restoration, Chinese Academy of Forestry/Grassland Research Center, National Forestry and Grassland Administration, Beijing 100091, China;
3. College of Grassland Science Inner Mongolia Agricultural University, Key Laboratory of Grassland Resources of the Ministry of Education, Hohhot, Inner Mongolia 010011, China

Received:2024-11-25 Revised:2025-02-25 Published:2025-11-13

野牛草BdGH3基因家族鉴定及干旱胁迫下的表达模式分析

王雪萍¹, 王思宁², 祁浩乐³, 张然², 李晓霞², 马晖玲¹

1. 甘肃农业大学草业学院, 草业生态系统教育部重点实验室, 甘肃省草业工程实验室, 中-美草地畜牧业可持续发展研究中心, 甘肃兰州 730070;
2. 中国林业科学研究院生态保护与修复研究所/国家林业和草原局草原研究中心, 北京 100091;
3. 内蒙古农业大学草业学院, 草地资源教育部重点实验室, 内蒙古呼和浩特 010011

通讯作者: 马晖玲,E-mail:mahl@gsau.edu.cn
作者简介:王雪萍（1997-），女，汉族，甘肃通渭人，硕士研究生，主要从事草类植物分子育种方面研究，E-mail：3540603407@qq.com
基金资助:
科技基础资源调查专项（2024FY100303-04）；中国林业科学研究院基本科研业务费专项资金项目（CAFYBB2022XA002）资助

Abstract

Abstract: Auxin-amido synthetases （Gretchen Hagen 3， GH3）， as early auxin-responsive genes， play a key role in regulating plant growth， development， and responses to abiotic stress. To identify GH3 genes in Buchloe dactyloides that respond to drought stress， this study used bioinformatics methods to identify 16 GH3 family genes in the genome of Buchloe dactyloides ‘honglinyu No.1’. The BdGH3 genes in B. dactyloides are unevenly distributed across 11 chromosomes. Motif and gene structure analyses revealed that most BdGH3 genes contain conserved motifs （Motif1 to Motif10） and 0 to 2 exons. Subcellular localization predictions indicated that fifteen BdGH3 proteins are localized in the chloroplasts. Phylogenetic tree analysis indicated that BdGH3 genes are clustered into three subfamilies. Cis-acting element analysis showed that BdGH3 genes contain multiple cis-acting elements related to light signals， hormone signals， and drought stress. Expression pattern analysis revealed that the expression levels of BdGH3.1 and BdGH3.15 in leaves， and BdGH3.5 and BdGH3.9 in roots， were significantly up-regulated throughout the drought stress period. The results of this study provide a theoretical reference for further functional research on BdGH3 genes and the drought-response mechanism in B. dactyloides.

Key words: Buchloe dactyloides, GH3 gene family identification, Drought stress, Expression pattern analysis

摘要： 生长素酰胺合成酶（Gretchen Hagen 3，GH3）作为生长素早期响应基因，在调控植物生长发育及逆境胁迫中扮演着关键角色。为挖掘野牛草（Buchloe dactyloides）响应干旱非生物胁迫的GH3基因，本研究利用生物信息学方法，在‘中林育1号’野牛草（Buchloe dactyloides ‘Zhonglinyu No.1’）基因组中鉴定出16个GH3家族基因。野牛草BdGH3基因在11条染色体上呈不均匀分布，保守基序及基因结构分析表明BdGH3基因多数含有Motif1~Motif10保守基序和0~2个外显子。15条BdGH3蛋白定位于叶绿体中。系统进化树分析表明BdGH3聚类为3个亚家族。顺式作用元件分析表明BdGH3基因含有多个响应光信号、激素信号和干旱等逆境相关的顺式作用元件。表达模式分析结果显示叶中BdGH3.1和BdGH3.15基因，根中BdGH3.5和BdGH3.9基因的表达量水平在整个干旱胁迫时期均表现为持续显著上调表达。本研究结果为进一步开展野牛草BdGH3基因的功能研究和耐旱响应机制提供理论参考。

关键词: 野牛草, GH3基因家族鉴定, 干旱胁迫, 表达模式分析

CLC Number:

Q786

WANG Xue-ping, WANG Si-ning, QI Hao-le, ZHANG Ran, LI Xiao-xia, MA Hui-ling. Identification of Buchloe Dactyloides BdGH3 Gene Family and Analysis of Expression Pattern under Drought Stress[J]. Acta Agrestia Sinica, 2025, 33(11): 3515-3525.

王雪萍, 王思宁, 祁浩乐, 张然, 李晓霞, 马晖玲. 野牛草BdGH3基因家族鉴定及干旱胁迫下的表达模式分析[J]. 草地学报, 2025, 33(11): 3515-3525.

References

[1] QIAN Y H，ZHANG Y Z，BAI P X，et al. Identification and expression analysis of GH3 family gene in Camellia sinensis[J]. Journal of Tea Communication，2020，47（3）：382-392 钱因红，张亚真，白培贤，等. 茶树GH3基因家族的鉴定及表达分析[J]. 茶叶通讯，2020，47（3）：382-392
[2] OSTROWSKI M，JAKUBOWSKA A. GH3 expression and IAA-amide synthetase activity in pea （Pisum sativum L.） seedlings are regulated by light，plant hormones and auxinic herbicides[J]. Journal of Plant Physiology，2013，170（4）：361-368
[3] KASAHARA H. Current aspects of auxin biosynthesis in plants[J]. Bioscience，Biotechnology，and Biochemistry，2016，80（1）：34-42
[4] STASWICK P E，TIRYAKI I，ROWE M L. Jasmonate response locus JAR1 and several related Arabidopsis genes encode enzymes of the firefly luciferase superfamily that show activity on jasmonic，salicylic，and indole-3-acetic acids in an assay for adenylation[J]. The Plant Cell，2002，14（6）：1405-1415
[5] FENG S G，YUE R Q，TAO S，et al. Genome-wide identification，expression analysis of auxin-responsive GH3 family genes in maize （Zea mays L.） under abiotic stresses[J]. Journal of Integrative Plant Biology，2015，57（9）：783-795
[6] POZARNSKY T. Buffalograss： Home on the range， but also a turfgrass[J]. Rangelands Archives，1983，5（05）：214-216
[7] ZHAO W，SHEN X J，GUO W，et al. Genome-wide analysis of GH3 gene family in soybean（Glycine max）[J]. Oil Crop Science，2016，1（2）：1-9
[8] ZHOU S Y，LI J M，MAO J. AtGH3.17-mediated regulation of auxin and brassinosteroid response in Arabidopsis thaliana[J]. Chinese Bulletin of Botany，2023，58（3）：373-384 周淑瑶，李建明，毛娟. AtGH3.17调控拟南芥生长素和油菜素甾醇的响应[J]. 植物学报，2023，58（3）：373-384
[9] ZHANG X X，MA Y Y，LUO X R，et al. Identification and evolutional and expression analysis of the GH3 gene family in kidney bean[J]. Acta Botanica Boreali-Occidentalia Sinica，2024，44（9）：1433-1444 张晓旭，马媛媛，罗新锐，等. 芸豆GH3基因家族鉴定及进化与表达分析[J]. 西北植物学报，2024，44（9）：1433-1444
[10] GUO X W，FU H B，ZHANG J Y，et al. Identification and expression analysis of the GH3 gene family in Cerasus humilis[J]. Molecular Plant Breeding，2023，21（6）：1842-1852 郭夕雯，付鸿博，张建英，等. 欧李GH3基因家族的鉴定及表达分析[J]. 分子植物育种，2023，21（6）：1842-1852
[11] LI Z L，HUANG K X，SUN Y. Salt-tolerance evaluation and analysis on Buffalo Grass germplasm resources[J]. Grassland and Prataculture，2022，34（2）：27-34 李智林，黄可心，孙彦. 野牛草种质资源耐盐性评价与筛选[J]. 草原与草业，2022，34（2）：27-34
[12] QIAO J Y，SUN X N，QU X Y，et al. Evaluation and analysis of buffalograss germplasm resources[J]. Acta Agrestia Sinica，2022，30（5）：1203-1209 乔葭月，孙熙喏，屈新月，等. 野牛草种质资源评价与分析[J]. 草地学报，2022，30（5）：1203-1209
[13] QIAN Y Q，LI D Y，HAN L，et al. Inter-ramet photosynthate translocation in buffalograss under differential water deficit stress[J]. Journal of the American Society for Horticultural Science，2010，135（4）：310-316
[14] LI Y，ZUO K J，TANG K X. A survey of functional studies of the GH3 gene family in plants[J]. Chinese Bulletin of Botany，2008，43（5）：507-515 黎颖，左开井，唐克轩. 植物GH3基因家族的功能研究概况[J]. 植物学通报，2008，43（5）：507-515
[15] YU D Q，QANMBER G，LU L L，et al. Genome-wide analysis of cotton GH3 subfamily II reveals functional divergence in fiber development，hormone response and plant architecture[J]. BMC Plant Biology，2018，18（1）：350
[16] YANG Y J，YUE R Q，SUN T，et al. Genome-wide identification，expression analysis of GH3 family genes in Medicago truncatula under stress-related hormones and Sinorhizobium meliloti infection[J]. Applied Microbiology and Biotechnology，2015，99（2）：841-854
[17] XU J F，ZHANG H H，JIN W B. Genome-wide identification，characterization，and expression profiles of auxin responsive GH3 gene family in Salvia miltiorrhiza involved in MeJA treatment[J]. Journal of Plant Biochemistry and Biotechnology，2022，31（1）：85-97
[18] JEONG J，PARK S，IM J H，et al. Genome-wide identification of GH3 genes in Brassica oleracea and identification of a promoter region for anther-specific expression of a GH3 gene[J]. BMC Genomics，2021，22（1）：22
[19] ZHANG D F，ZHANG N，ZHONG T，et al. Identification and characterization of the GH3 gene family in maize[J]. Journal of Integrative Agriculture，2016，15（2）：249-261
[20] KHAN S，STONE J M. Arabidopsis thaliana GH3.9 in auxin and jasmonate cross talk[J]. Plant Signaling & Behavior，2007，2（6）：483-485
[21] MA L L，WANG Q，ZHENG Y Y，et al. Cucurbitaceae genome evolution，gene function and molecular breeding[J]. Horticulture Research，2022，9：uhab057
[22] GUO L L. Identification of auxin response factor GH3 family in Fragaria and functional study of FaGH3.17 and FaGH3.18 in response to drought[D]. Lanzhou：Gansu Agricultural University，2023：5-7 郭丽丽. 草莓属生长素响应因子GH3家族的鉴定及FaGH3.17、FaGH3.18响应干旱的功能研究[D]. 兰州：甘肃农业大学，2023：5-7
[23] JAIN M，KAUR N，TYAGI A K，et al. The auxin-responsive GH3 gene family in rice （Oryza sativa）[J]. Functional & Integrative Genomics，2006，6（1）：36-46
[24] BAO D F，CHANG S Q，LI X D，et al. Advances in the study of auxin early response genes： Au_x/IAA GH3 and SAUR[J]. The Crop Journal，2024，12（4）：964-978
[25] WOJTACZKA P，CIARKOWSKA A，STARZYNSKA E，et al. The GH3 amidosynthetases family and their role in metabolic crosstalk modulation of plant signaling compounds[J]. Phytochemistry，2022，194：113039
[26] YU J D，LI Y Z，MIAO J M，et al. Cloning and expression pattern analysis of BBML gene in Poa pratensis（Kentucky bluegrass）[J]. Acta Agrestia Sinica，2024，32（2）：396-408 余江弟，李玉珠，苗佳敏，等. 草地早熟禾BBML基因的克隆及表达模式分析[J]. 草地学报，2024，32（2）：396-408
[27] WESTFALL C S，SHERP A M，ZUBIETA C，et al. Arabidopsis thaliana GH3.5 acyl acid amido synthetase mediates metabolic crosstalk in auxin and salicylic acid homeostasis[J]. Proceedings of the National Academy of Sciences of the United States of America，2016，113（48）：13917-13922
[28] SMOLKO A，JÜLKE S，BENADE F，et al. The GH3 protein from the clubroot pathogen Plasmodiophora brassicae causes hormone-related phenotypes in Arabidopsis thaliana[J]. Journal of Plant Growth Regulation，2024，43（8）：2715-2738
[29] HOU Q D. Tissue specific expression of ARF，AUX/IAA and GH3 in sweet cherry （Prunus avium L.） and functional analysis of PavARF4[D]. Guiyang：Guizhou University，2021：4-6 侯黔东. 甜樱桃ARF、AUX/IAA及GH3组织特异性表达与PavARF4功能分析[D]. 贵阳：贵州大学，2021：4-6
[30] LIU Y Y，YANG M，TANG M H，et al. Identification and analysis of MDHAR gene family members in tea plants[J]. Grain Oil and Feed Technology，2023（2）：207-209 刘益溢，杨梅，唐木花，等. 茶树MDHAR基因家族成员的鉴定与分析[J]. 粮油与饲料科技，2023（2）：207-209
[31] FEI L H，et al. High expression of pituitary tumor gene family is a predictor for poor prognosis of gastric cancer[J]. All Life，2022，15（1）：820-833
[32] GAN Z Y，FEI L Y，SHAN N，et al. Identification and expression analysis of gretchen Hagen 3 （GH3） in kiwifruit （Actinidia chinensis） during postharvest process[J]. Plants，2019，8（11）：473
[33] WEI L J，YANG B，JIAN H J，et al. Genome-wide identification and characterization of Gretchen Hagen3 （GH3） family genes in Brassica napus[J]. Genome，2019，62（9）：597-608
[34] DU H，WU N，FU J，et al. A GH3 family member，OsGH3-2，modulates auxin and abscisic acid levels and differentially affects drought and cold tolerance in rice[J]. Journal of Experimental Botany，2012，63（18）：6467-6480
[35] CHEN X. The roles and regulatory mechanisms of a putative tomato indole-3-acetic acid amido synthetase gene，Slgh3.4，in the establishment of arbuscular mycorrhizal symbiosis[D]. Nanjing：Nanjing Agricultural University，2017：3-4 陈潇. 番茄吲哚-3-乙酸氨基合成酶基因SlGH3.4在丛枝菌根共生中的功能和调控机制研究[D]. 南京：南京农业大学，2017：3-4
[36] WANG X G，JIA C Y，AN L S，et al. Genome-wide identification and expression characterization of the GH3 gene family of tea plant （Camellia sinensis）[J]. BMC Genomics，2024，25（1）：120
[37] MATHURA S R，SUTTON F，BOWRIN V. Genome-wide identification，characterization，and expression analysis of the sweet potato （Ipomoea batatas [L.] Lam.） ARF Au_x/IAA GH3 and SAUR gene families[J]. BMC Plant Biology，2023，23（1）：622
[38] ZOU W H，LIN P X，ZHAO Z N，et al. Genome-wide identification of auxin-responsive GH3 gene family in Saccharum and the expression of ScGH3-1 in stress response[J]. International Journal of Molecular Sciences，2022，23（21）：12750
[39] ZHU D，WANG B B，HE M M. Identification and expression analysis of GH3 gene family members in potato（Solanum tuberosum）[J]. Journal of Agricultural Biotechnology，2025，33（3）：498-512 朱迪，王冰冰，贺苗苗. 马铃薯GH3基因家族成员的鉴定及表达分析[J]. 农业生物技术学报，2025，33（3）：498-512

Identification of Buchloe Dactyloides BdGH3 Gene Family and Analysis of Expression Pattern under Drought Stress

野牛草BdGH3基因家族鉴定及干旱胁迫下的表达模式分析

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