Cloning， Expression Pattern Analysis and Subcellular Localization of LcPDS gene in Leymus chinensis

doi:10.11733/j.issn.1007-0435.2025.11.007

Acta Agrestia Sinica ›› 2025, Vol. 33 ›› Issue (11): 3550-3558.DOI: 10.11733/j.issn.1007-0435.2025.11.007

Cloning， Expression Pattern Analysis and Subcellular Localization of LcPDS gene in Leymus chinensis

WANG Jie-fan¹, LI Jian-hui², ZHANG Sheng-yuan², DONG Di³, ZHANG Ran³, WANG Si-ning³, LIU Yue-han⁴, LI Xiao-xia³, SHI Feng-ling¹

1. Key Laboratory of Grassland Resources of Ministry of Education, College of Grassland Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, China;
2. Qinghai Sanjiang Group Co., Ltd, Xining, Qinhai 810000, China;
3. Institute of Ecological Conservation and Restoration, Chinese Academy of Forestry, Beijing 100091, China;
4. Grassland Research and Technology Promotion Department, Xinjiang Uygur Autonomous Region Grassland Station, Urumqi, Xinjiang 830000, China

Received:2024-11-11 Revised:2025-02-23 Published:2025-11-13

羊草LcPDS基因的克隆、表达模式分析及亚细胞定位

王介繁¹, 李健辉², 张生原², 董笛³, 张然³, 王思宁³, 刘岳含⁴, 李晓霞³, 石凤翎¹

1. 内蒙古农业大学草业学院草地资源教育部重点实验室, 内蒙古呼和浩特 010018;
2. 青海省三江集团有限责任公司, 青海西宁 810000;
3. 中国林业科学研究院生态保护与修复研究所, 北京 100091;
4. 新疆维吾尔自治区草原总站草原研究与技术推广科, 新疆乌鲁木齐 830000

通讯作者: 石凤翎,E-mail:shifengling@imau.edu.cn
作者简介:王介繁（1999-），女，汉族，河北玉田人，硕士研究生，主要从事牧草育种研究，E-mail：2593306613@qq.com
基金资助:
国家自然科学基金面上项目“变温调控羊草种子萌发的分子机制研究”（32071869）；中国林业科学研究院基本科研业务费专项资金项目（CAFYBB2022XA002）资助

Abstract

Abstract: Phytoene desaturase （PDS）， which affects the biosynthesis of carotenoids， is a key enzyme in the synthetic pathway. In order to study the function of the PDS gene of Leymus chinensis， in this experiment， the PDS gene was successfully cloned from ‘Zhongke No.1’ L. chinensis and bioinformatics analysis was performed. Protein structure analysis， subcellular localization， transcriptional self-activation detection and expression pattern analysis were conducted as well. The results showed that the full-length cDNA sequence of this gene is 1743 bp， encoding 580 amino acids. Chloroplast transit peptides may exist in the LcPDS protein， and the the protein has the closest relationship with the PDS protein of wheat （Triticum aestivum）. Subcellular localization revealed that the protein is localized in the chloroplast. The transcriptional self-activation test showed that LcPDS does not have self-activation. There was no significant difference in the LcPDS gene expression among different developmental stages of leaves. The expression levels in various organs followed the order： root > leaf > stem （P<0.05）. The LcPDS gene is regulated by gibberellin， abscisic acid， methyl jasmonate and salicylic acid. This study provides a scientific foundation for further research on the function of the LcPDS gene.

Key words: Leymus chinensis, PDS gene, Bioinformatics, Analysis of expression patterns

摘要： 八氢番茄红素脱氢酶（Phytoene desaturase， PDS）影响类胡萝卜素的合成，是其合成途径中的关键酶。为了研究羊草（Leymus chinensis）PDS基因的功能，本试验从‘中科1号’羊草中成功克隆出PDS基因并进行了生物信息学分析、蛋白结构分析、亚细胞定位、转录自激活检测及表达模式分析。结果显示，该基因全长cDNA序列为1743 bp，编码580个氨基酸。LcPDS蛋白可能存在叶绿体导肽，LcPDS蛋白与小麦（Triticum aestivum）PDS蛋白的关系最密切。亚细胞定位显示该蛋白定位于叶绿体上。转录自激活试验显示LcPDS不具备自激活性。LcPDS基因在叶片的不同发育阶段间并无显著差异，在不同器官中表达量为根>叶>茎（P<0.05）。LcPDS基因受赤霉素、脱落酸、茉莉酸甲酯以及水杨酸4种激素调控。本研究为进一步研究LcPDS基因功能提供了科学依据。

关键词: 羊草, PDS基因, 生物信息学, 表达模式分析

CLC Number:

S543.9

WANG Jie-fan, LI Jian-hui, ZHANG Sheng-yuan, DONG Di, ZHANG Ran, WANG Si-ning, LIU Yue-han, LI Xiao-xia, SHI Feng-ling. Cloning， Expression Pattern Analysis and Subcellular Localization of LcPDS gene in Leymus chinensis[J]. Acta Agrestia Sinica, 2025, 33(11): 3550-3558.

王介繁, 李健辉, 张生原, 董笛, 张然, 王思宁, 刘岳含, 李晓霞, 石凤翎. 羊草LcPDS基因的克隆、表达模式分析及亚细胞定位[J]. 草地学报, 2025, 33(11): 3550-3558.

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Cloning， Expression Pattern Analysis and Subcellular Localization of LcPDS gene in Leymus chinensis

羊草LcPDS基因的克隆、表达模式分析及亚细胞定位

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