Effect of Fertilization on Soil Fungal Community of Elymus nutans ‘Baqing’ Grassland

doi:10.11733/j.issn.1007-0435.2025.11.011

Acta Agrestia Sinica ›› 2025, Vol. 33 ›› Issue (11): 3593-3602.DOI: 10.11733/j.issn.1007-0435.2025.11.011

Effect of Fertilization on Soil Fungal Community of Elymus nutans ‘Baqing’ Grassland

LEI Bian-xia¹, MIAO Yan-jun², ZHENG Shao-long³, WANG Chuan-qi¹, WANG Ming-tao¹

1. Gansu Forestry Voctech University, Tianshui, Gansu Province 741020, China;
2. Xizang Agriculture and Animal Husbandry University, Nyingchi, Xizang 860000, China;
3. Naiman Banner Forestry and Grassland Bureau of Tongliao City, Tongliao Inner Mongolia 028300, China

Received:2024-11-26 Revised:2024-12-24 Published:2025-11-13

施肥对‘巴青’垂穗披碱草草地土壤真菌群落的影响

雷变霞¹, 苗彦军², 郑少龙³, 王传旗¹, 王明涛¹

1. 甘肃林业职业技术大学, 甘肃天水 741020;
2. 西藏农牧学院, 西藏林芝 860000;
3. 内蒙古通辽市奈曼旗林业和草原局, 内蒙古通辽 028300

通讯作者: 王明涛,E-mail:wmt060708@126.com
作者简介:雷变霞（1990-），女，汉族，甘肃定西人，硕士研究生，主要从事牧草及饲料作物栽培方面的研究，E-mail：Wlbx1990@126.com；
基金资助:
甘肃省教育厅：高校教师创新基金项目（2024B-254、2024B-261）；甘肃省青年人才团队项目（2025QNTD14）资助

Abstract

Abstract: To explore the effect of fertilization on soil fungal community of native plants， and to provide theoretical basis rational cultivation and breeding of wild forage germplasm resources in alpine region， we conducted a comprehensive analysis of fungal communities under different treatments： control （N₀P₀K₀）， nutrient-deficient zones （N₀P₂K₂， N₂P₀K₂， N₂P₂K₀）， and full fertilization zone （N₂P₂K₂） using Elymus nutans ‘Baqing’ grassland soil as the research subject， The results showed that the number of unique amplicon sequence variants （ASVs） in N₂P₂K₀ treatment increased after fertilization. The fertilizer-rich zone exhibited significantly reduced Shannon （P<0.05） and Pielou evenness indices （P<0.05） relative to control. Notably， N₂P₂K₀ treatment showed significantly higher richness and Shannon diversity than fertilizer-rich zone （P<0.05）. Soil available nitrogen （P<0.05） and soil pH （P<0.01） significantly affected species change at phylum level， and soil available phosphorus was a significant factor influencing species change at genus level （P<0.05）. Co-occurrence network analysis identified 9 keystone ASV nodes distributing across Ascomycota （5）， Basidiomycota （3）， and Mortierellomycota （1）. In conclusion， the fertilizer-rich zone significantly reduced the Shannon diversity and Pielou evenness indices of fungal communities. Compared to the fertilizer-rich zone， the combined nitrogen-phosphorus application demonstrated greater efficacy in enhancing species richness and Shannon diversity index. The identified keystone taxa provide critical insights for understanding fungal community assembly mechanisms and functional prediction in alpine ecosystems.

Key words: Cultivated grassland, Soil nutrients, Microbial community diversity, Redundancy analysis, Co-occurrence network

摘要： 探究施肥对乡土植物土壤真菌群落的影响，为高寒地区野生牧草种质资源的合理栽培和繁育提供理论依据。本研究以‘巴青’垂穗披碱草（Elymus nutans ‘Baqing’）草地的土壤真菌群落为研究对象，分析不同施肥处理[对照（N₀P₀K₀）、缺素区（N₀P₂K₂、N₂P₀K₂、N₂P₂K₀）、全肥区（N₂P₂K₂）]对土壤真菌群落的影响。结果表明：施肥后N₂P₂K₀处理独有扩增子序列变异体（ASVs）数量有升高的趋势。相比对照，全肥区真菌群落的香农多样性指数和均匀度指数显著下降（P<0.05）。缺素区N₂P₂K₀处理丰富度指数和香农多样性指数显著高于全肥区（P<0.05）。土壤速效氮（P<0.05）和土壤pH（P<0.01）是群落门水平物种变化的显著影响因子；属水平物种变化的显著影响因子为土壤速效磷（P<0.05）。共现网络分析显示，9个关键节点ASVs分别隶属于子囊菌门（5个）、担子菌门（3个）和被孢霉门（1个）。综上所述，全肥区显著降低了真菌群落的香农多样性和均匀度指数，相较全肥区对真菌群落多样性的影响，氮磷共施更有利于丰富度和香农多样性指数提升。关注共现网络中的关键节点菌属对进一步研究土壤真菌群落组装过程及其功能预测具有指导意义。

关键词: 栽培草地, 土壤养分, 微生物群落多样性, 冗余分析, 共现网络

CLC Number:

S812.2

LEI Bian-xia, MIAO Yan-jun, ZHENG Shao-long, WANG Chuan-qi, WANG Ming-tao. Effect of Fertilization on Soil Fungal Community of Elymus nutans ‘Baqing’ Grassland[J]. Acta Agrestia Sinica, 2025, 33(11): 3593-3602.

雷变霞, 苗彦军, 郑少龙, 王传旗, 王明涛. 施肥对‘巴青’垂穗披碱草草地土壤真菌群落的影响[J]. 草地学报, 2025, 33(11): 3593-3602.

References

[1] LONG J T，GAO X L，BAO S，et al. Research progress on stress resistance of Elymus nutans[J]. Journal of Grassland and Forage Science，2022（3）：1-8 龙建廷，高献磊，包赛很那，等. 垂穗披碱草抗逆性研究进展[J]. 草学，2022（3）：1-8
[2] ZHOU X L，MA K，WANG Y C，et al. Effects of organic fertilizer on soil microflora in degraded grassland around Qinghai Lake[J]. Acta Agrestia Sinica，2022，30（12）：3191-3198 周学丽，马坤，王英成，等. 有机肥对环青海湖地区退化草地土壤微生物区系的影响[J]. 草地学报，2022，30（12）：3191-3198
[3] ZHANG X F，ZHANG C P，DONG Q M，et al. Soil fungal community structure in perennial cultivated grassland around Qinghai lake[J]. Acta Agrestia Sinica，2024，32（9）：2803-2815 张小芳，张春平，董全民，等. 环青海湖地区多年生栽培草地土壤真菌群落结构特征[J]. 草地学报，2024，23（9）：2803-2815
[4] SUN P，ZHANG S X，WU Q H，et al. pH and ammonium concentration are dominant predictors of the abundance and community composition of comammox bacteria in long-term fertilized Mollisol[J]. Applied Soil Ecology，2021，168：104139
[5] HU X J，GU H D，LIU J J，et al. High variation of fungal communities and associated potential plant pathogens induced by long-term addition of N fertilizers rather than P， K fertilizers： A case study in a Mollisol field[J]. Soil Ecology Letters，2022，4（4）：348-361
[6] HU X X，LIU J J，WEI D，et al. Effects of over 30-year of different fertilization regimes on fungal community compositions in the black soils of northeast China[J]. Agriculture， Ecosystems & Environment，2017，248：113-122
[7] WANG G H，HU X J，YU Z H，et al. Effects of fertilization on soil microbial community diversity in Chinese arable black soils： research progress and prospects[J]. Soils and Crops，2024，13（2）：127-139 王光华，胡晓婧，于镇华，等. 施肥对我国黑土农田土壤微生物群落多样性影响的研究及展望[J]. 土壤与作物，2024，13（2）：127-139
[8] ZHANG B，LI J R，GUO L N，et al. Research progress on the effect of organic fertilizer application on soil microorganisms[J]. Journal of Jilin Forestry Science and Technology，2024，53（6）：27-32 张波，李江荣，郭亮娜，等. 有机肥添加对土壤微生物的影响研究进展[J]. 吉林林业科技，2024，53（6）：27-32
[9] LIU K S，SHAO X Q. Grassland ecological management and utilization for sustainability[J]. Agronomy，2024，14（1）：149
[10] ZHANG Y，XIE Y Z，MA H B，et al. Responses of soil microorganisms to simulated climate change in desert grassland in northern China[J]. Journal of Water and Climate Change，2022，13（4）：1842-1854
[11] JIA X. Resistance and adaptation of Tibet wild Elymus nutans to low temperature stress[D]. Lhasa：Tibet University，2021：24-25 贾祥. 西藏野生垂穗披碱草对低温胁迫的抗性和适应[D]. 拉萨：西藏大学，2021：24-25
[12] HU T M，MIAO Y J. Review on reproduction of wild forages in Xizang[J]. Journal of Grassland and Forage Science，2020（2）：75-79 呼天明，苗彦军. 西藏野生优质牧草繁育技术研究述评[J]. 草学，2020（2）：75-79
[13] BAO S，MIAO Y J，GUO Y L，et al. Application study on ecological bag technology of restoring slope in high-grade highways in Nyingchi，Xizang[J]. Science of Soil and Water Conservation，2019，17（3）：140-147 包赛很那，苗彦军，郭云雷，等. 西藏林芝地区高等级公路生态袋边坡修复技术应用研究[J]. 中国水土保持科学，2019，17（3）：140-147
[14] SONG J C. Effect of combined application of nitrogen， phosphorus andpotassium fertilizer on seed yield， quality of Elymus nutansand soil properties of grassland in alpine region. Lanzhou：Gansu Agricultural University，2022：29-30 宋建超. 氮磷钾肥配施对高寒区垂穗披碱草种子产量、质量和土壤特性的影响[D]. 兰州：甘肃农业大学，2022：29-30
[15] WANG Z Q，WANG W Z，MA Z，et al. Effects of different grass growth on soil nutrients and microbial community of Pedicularis kansuensis community[J]. Acta Agrestia Sinica，2023，31（12）：3750-3758 王占青，王午志，马喆，等. 不同禾草生长对甘肃马先蒿群落土壤养分和微生物群落的影响[J]. 草地学报，2023，31（12）：3750-3758
[16] CALLAHAN B J，MCMURDIE P J，ROSEN M J，et al. Dada2：high-resolution sample inference from illumina amplicon data[J]. Nature Methods，2016，13（7）：581-583
[17] KOLJALG U，NILSSON R H，ABARENKOV K，et al. Towards a unified paradigm for sequence-based identification of fungi[J]. Molecular Ecology，2013，22：5271-5277
[18] YUE H，BANERJEE S，LIU C，et al. Fertilizing-induced changes in the nitrifying microbiota associated with soil nitrification and crop yield[J]. Science of the Total Environment，2022，841：156752
[19] ZHONG Y， SORENSEN P O， ZHU G， et al. Differential microbial assembly processes and co‐occurrence networks in the soil‐root continuum along an environmental gradient[J]. iMeta，2022，1（2）：e18
[20] ZHAO Y H，WANG M T，YANG Y Y，et al. Tillage promotes the migration and coexistence of bacteria communities from an agro-pastoral ecotone of Xizang[J]. Microorganisms，2022，10（6）：1206
[21] FENG Z，LI N，DENG Y，et al. Biogeography and assembly processes of abundant and rare soil microbial taxa in the southern part of the Qilian Mountain National Park， China[J]. Ecology and Evolution，2024，14（2）：e11001
[22] MA K，LU G X，DENG Y，et al. Effects of applying organic fertilizer on rhizosphere microorganisms and soil physical and chemical properties of Elymus nutans[J]. Acta Agrestia Sinica，2022，30（3）：594-602 马坤，芦光新，邓晔，等. 有机肥对垂穗披碱草根际微生物及土壤理化性质的影响[J]. 草地学报，2022，30（3）：594-602
[23] XU C Y. Study on effects of biochar and organic fertilizer applied to wheat-maize rotation in sandy fluvo-aquic soil[D]. Zhengzhou：Henan Agricultural University，2024：48-49 徐春燕. 砂质潮土小麦玉米轮作施用生物炭和有机肥效应研究[D]. 郑州：河南农业大学，2024：48-49
[24] ZHAO S H，LI Y F，WANG Z. Spectral characteristics of summer maize and the responses of its grain yield to nitrogen and phosphorus fertilization under drip irrigation[J]. Journal of Irrigation and Drainage，2024，43（7）：19-28 赵双会，栗岩峰，王珍. 夏玉米光谱特征和产量对滴灌氮磷调控的响应[J]. 灌溉排水学报，2024，43（7）：19-28
[25] LI H，SU M D，HUANG L P，et al. Effects of nitrogen，phosphorus and potassium application on available nutrients and fungal diversity in tobacco-growing soil[J]. Crops，2023（3）：238-245 李航，苏梦迪，黄浪平，等. 氮磷钾配施对植烟土壤速效养分和真菌多样性的影响[J]. 作物杂志，2023（3）：238-245
[26] LI M，DENG Q Z，JING Y T，et al. Distribution characteristics and driving factors of soil fungi between alfalfa roots in different habitats in northern Tianshan Mountains[J]. Arid Land Geography，2024，47（7）：1187-1198 李嫚，邓錡璋，景玉婷，等. 天山北部不同生境苜蓿根际土壤真菌分布特征及驱动因子[J]. 干旱区地理，2024，47（7）：1187-1198
[27] YANG K，DU Y Q，ZHANG X X，et al. Effects of different organic materials combined with chemical fertilizers on soil fungal community structure and ecological function[J]. Soils，2024，56（1）：222-228 杨凯，杜延全，张西兴，等. 不同有机物料与化肥配施对土壤真菌群落结构和生态功能的影响[J]. 土壤，2024，56（1）：222-228
[28] XU W H. Effects of warming on surface and deep soil organic carbon components of Chinese fir plantations[D]. Hefei：Anhui Agricultural University，2023：4-5 胥文昊. 增温对杉木人工林表层和深层土壤有机碳组分的影响[D]. 合肥：安徽农业大学，2023：4-5
[29] FREITAS J F，OLIVEIRA T T，AGNEZ-LIMA L F. Metaviromic reveals the dynamics and diversity of the virosphere in wastewater samples from Natal，Brazil[J]. Environmental Pollution，2024，59：124752
[30] CHEN J，NAN J，XU D L，et al. Response differences between soil fungal and bacterial communities under opencast coal mining disturbance conditions[J]. Catena，2020，194：104779
[31] CHENG H P，SUO B Y，WANG F，et al. Effects of hydrogen sulfide on the structures of phyllosphere and rhizosphere microbial communities of soybean plants under drought stress[J]. Acta Microbiologica Sinica，2024，64（11）：4171-4189 程华平，索冰玉，王冯姝雅，等. 硫化氢对水分逆境胁迫下大豆叶际和根内微生物群落结构的影响[J]. 微生物学报，2024，64（11）：4171-4189
[32] XUE M，ZHANG R R，ZHANG Y，et al. Diversity and extracellular enzyme activities of Rhodotorula strains isolated from surface ice core of the Glacier No.1 at headwaters of Urumqi River，Tianshan Mountains[J]. Journal of Glaciology and Geocryology，2020，42（4）：1308-1320 薛梅，张瑞蕊，张艳，等. 天山乌鲁木齐河源1号冰川浅冰芯中红酵母菌多样性及种系的产酶特性分析[J]. 冰川冻土，2020，42（4）：1308-1320
[33] GAO H L，CHEN H M，LING Y，et al. Clinical analysis of 51 cases with cryptococcal meningitis[J]. Journal of Brain and Nervous Diseases，2021，29（5）：284-287 高红林，陈慧敏，凌勇，等. 隐球菌性脑膜炎51例临床特点分析[J]. 脑与神经疾病杂志，2021，29（5）：284-287

Effect of Fertilization on Soil Fungal Community of Elymus nutans ‘Baqing’ Grassland

施肥对‘巴青’垂穗披碱草草地土壤真菌群落的影响

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