Effects of Allelopathic α-Pinene on Feedback Interactions of Elymus Nutans and Soil

doi:10.11733/j.issn.1007-0435.2025.11.023

Acta Agrestia Sinica ›› 2025, Vol. 33 ›› Issue (11): 3722-3731.DOI: 10.11733/j.issn.1007-0435.2025.11.023

Effects of Allelopathic α-Pinene on Feedback Interactions of Elymus Nutans and Soil

CHEN Meng-ci¹, SHENG Ting-yan², Suolang Daji³, QUAN Xiao-long¹, SHI Hui-lan⁴, QIAO You-ming¹

1. State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, Qinghai Province 810016, China;
2. Qinghai Agriculture and Animal Husbandry Science and Technology Vocational College, Huangyuan, Qinghai Province 812100, China;
3. Agricultural and Animal Husbandry Comprehensive Service Center of Haibei Tibetan Autonomous Prefecture, Haibei, Qinghai Province 810299, China;
4. College of Eco-environmental Engineering, Qinghai University, Xining, Qinghai Province 810016, China

Received:2024-12-26 Revised:2025-02-16 Published:2025-11-13

化感物质α-蒎烯对垂穗披碱草-土壤反馈作用的影响

陈梦词¹, 盛庭岩², 索郎大吉³, 全小龙¹, 史惠兰⁴, 乔有明¹

1. 青海大学省部共建三江源生态与高原农牧业国家重点实验室, 青海西宁 810016;
2. 青海农牧科技职业学院, 青海湟源 812100;
3. 海北藏族自治州农牧综合服务中心, 青海海北 810299;
4. 青海大学生态环境工程学院, 青海西宁 810016

通讯作者: 乔有明,E-mail:ymqiao@aliyun.com
作者简介:陈梦词（1989-），女，汉族，青海西宁人，博士，实验师，主要从事草地生态与环境保护的相关研究，E-mail：chenmengci@126.com
基金资助:
省部共建三江源生态与高原农牧业国家重点实验室自主课题项目（2025-ZZ-02）；第四期青海省“昆仑英才·高端创新创业人才”计划资助

Abstract

Abstract: The α-pinene is a main allelochemical of major noxious weeds in the alpine grassland of Sangjiangyuan， which can inhibit the growth of Elymus nutans， an important forage. However， the response changes of α-pinene-induced allelopathy to soil characteristics of E. nutans and seedling growth is still unclear. In this study， α-pinene， E. nutans native soil and seeds were used as research materials. BIOLOG technology was used to determine the allelopathic effects of α-pinene on soil property， microbial related index， seed germination， seedling height， root length and other growth indicators of E. nutans. The results showed that after the soil was treated with α-pinene， soil nitrogen supply was improved over time. The α-pinene and soil microorganisms had feedback. The α-pinene has a negative hysteresis effect on soil microorganisms. And after α-pinene treatment， the intensity of carbon source utilization by soil microorganisms had weakened with the extension of relief time. Soil microorganisms responded positively to allelopathic effects of α-pinene by changing amino acids and carbohydrate utilization capacity of carbon sources. The feedback effect between α-pinene and soil microorganisms was also found in the E. nutans seedlings. The α-pinene treated soil inhibited the average root length of the seedlings， and soil microorganisms could alleviate the allelopathic inhibition of α-pinene. These results provide a reference for further understanding of the allelopathic mechanisms of the important allelochemical of noxious weeds on E. nutans in the artificial grassland produced.

Key words: Allelopathy, BIOLOG, Soil nutrients, Microorganisms, Alleviation

摘要： α-蒎烯（α-pinene）是三江源高寒草地几种主要毒杂草释放的可抑制优良牧草垂穗披碱草（Elymus nutans）的重要化感物质，但α-蒎烯对垂穗披碱草土壤特性及其幼苗生长的影响尚未明确，特别是不同缓解时间下的作用效果仍不清楚。本研究以α-蒎烯、垂穗披碱草原生土壤及垂穗披碱草种子为研究材料，利用BIOLOG技术，测定了单一化感物质对土壤化学性质、微生物相关指标和垂穗披碱草种子发芽、幼苗苗高、根长等相关生长指标的影响。结果表明：α-蒎烯处理垂穗披碱草土壤后，随着缓解时间的延长，土壤氮素供应情况得到改善；α-蒎烯与土壤微生物具有相互反馈的作用，α-蒎烯对土壤微生物产生负面影响，对微生物活性具有滞后性的抑制作用，且随着缓解时间的延长土壤微生物碳源利用强度减弱，而土壤微生物通过改变碳源中氨基酸类利用能力对α-蒎烯的化感作用产生积极响应；α-蒎烯与土壤微生物的反馈作用同样表现在垂穗披碱草幼苗中，α-蒎烯处理土壤后在幼苗根部表现出抑制作用，且土壤微生物的存在能够缓解α-蒎烯的化感抑制作用。上述研究结果为深入了解毒杂草的重要化感物质对垂穗披碱草人工草地产生的化感作用机制提供参考。

关键词: 化感作用, BIOLOG, 土壤养分, 微生物, 缓解作用

CLC Number:

S812

CHEN Meng-ci, SHENG Ting-yan, Suolang Daji, QUAN Xiao-long, SHI Hui-lan, QIAO You-ming. Effects of Allelopathic α-Pinene on Feedback Interactions of Elymus Nutans and Soil[J]. Acta Agrestia Sinica, 2025, 33(11): 3722-3731.

陈梦词, 盛庭岩, 索郎大吉, 全小龙, 史惠兰, 乔有明. 化感物质α-蒎烯对垂穗披碱草-土壤反馈作用的影响[J]. 草地学报, 2025, 33(11): 3722-3731.

References

[1] WANG C T，LONG R J，CAO G M. Soil carbon and nitrogen contents along elevation gradients in the source region of Yangtze， Yellow and Lantsang rivers[J]. Journal of Plant Ecology，2006，30（3）：441-449
[2] WANG G X， CHENG G D， SHEN Y P. Causes of grassland degradation and ecological restoration in the Three Rivers Source Region[J]. Acta Ecologica sinica， 2024，24（6）：1235-1243 王根绪，程国栋，沈永平. 三江源地区草地退化的原因及生态恢复对策[J]. 生态学报，2004，24（6）：1235-1243
[3] SHANG Z H，DONG Q M，SHI J J，et al. Research progress in recent ten years of ecological restoration for ‘Black Soil Land’ degraded grassland on Tibetan Plateau——concurrently discuss of ecological restoration in Sangjiangyuan Region[J]. Acta Agrestia Sinica，2018，26（1）：1-21 尚占环，董全民，施建军，等. 青藏高原“黑土滩”退化草地及其生态恢复近10年研究进展——兼论三江源生态恢复问题[J]. 草地学报，2018，26（1）：1-21
[4] ZHANG BC， BAI XF， GU LH， et al. Study on allelopathy and natural degeneration phenomena of artificial grassland on alpine meadow[J]. Acta Ecologica Sinica， 1989（2）：115-120 张宝琛，白雪芳，顾立华，等. 生化他感作用与高寒草甸上人工草场自然退化现象的研究[J]. 生态学报，1989，（2）：115-120
[5] GUO L B，QIU J，YE C Y，et al. Echinochloa crus-galli genome analysis provides insight into its adaptation and invasiveness as a weed[J]. Nature Communications，2017，8（1）：1031
[6] SHANG Z H， LONG R J， MA Y S， et al. Spatial heterogeneity and similarity of adult plants and seedlings in ‘black soil land’ secondary weed community， Qinghai-Tibetan Plateau[J]. Journal of Plant Ecology， 2008，32（5）：1157-1165 尚占环，龙瑞军，马玉寿，等. 青藏高原“黑土滩”次生毒杂草群落成体植株与幼苗空间异质性及相似性分析[J]. 植物生态学报，2008，（5）：1157-1165
[7] WANG F Y. Screening and evaluation of excellent germplasm of wild Elymus nutans in alpine region[D]. Xining：Qinghai University，2024：8 王凤宇. 高寒区野生垂穗披碱草优异种质筛选及评价[D]. 西宁：青海大学，2024：8
[8] LONG J T，GAO X L，BAO S，et al. Research progress on stress resistance of Elymus nutans[J]. Journal of Grassland and Forage Science，2022（3）：1-8 龙建廷，高献磊，包赛很那，等. 垂穗披碱草抗逆性研究进展[J]. 草学，2022（3）：1-8
[9] REN Y D， SHANG Z H， LONG R J. Progress of allelopathy in grassland ecosystem of China[J]. Pratacultural Science， 2014，31（5）：993-1002 任元丁，尚占环，龙瑞军. 中国草地生态系统中的化感作用研究进展[J]. 草业科学，2014，31（5）：993-1002
[10] LONG J T，GAO X L，BAO S，et al. Research progress on stress resistance of Elymus nutans[J]. Journal of Grassland and Forage Science，2022（3）：1-8 甄润德，张树源，白雪芳，等. 细叶亚菊挥发油中抑制垂穗披碱草的化合物的分离与鉴定[J]. 植物生理学报，1996，（3）：311-314
[11] QUAN X L. Allelopathic potential of cultivated grasses and main weeds in sown grassland of alpine regions[D]. Xining：Qinghai University，2022：19-27 全小龙. 高寒地区栽培牧草与人工草地主要杂草化感潜力分析[D]. 西宁：青海大学，2022：19-27
[12] ALLENSPACH M， VALDER C， FLAMM D，et al. Verification of chromatographic profile of primary essential oil of Pinus sylvestris L. combined with chemometric analysis[J]. Molecules，2020，25（13）：2973
[13] ADLARD E R. Handbook of essential oils. science， technology and applications[J]. Chromatographia， 2010，72（9-10）：1021
[14] KAMAL J. Allelopathy； a brief review[J]. Jordan Journal of Applied Science，2020：2020-2029
[15] VESPERMANN K A C，PAULINO B N， BARCELOS M C S，et al. Biotransformation of alpha- and beta-pinene into flavor compounds[J]. Applied Microbiology and Biotechnology，2017，101（5）：1805-1817
[16] WESTON L A，DUKE S O. Weed and crop allelopathy[J]. Critical Reviews in Plant Sciences，2003，22（3-4）：367-389
[17] MOVAHHED HAGHIGHI T，SAHARKHIZ M J，NADDAF F. Ontogenetic variability of Vitex pseudo-negundo essential oil and its phytotoxic activity[J]. Scientia Horticulturae，2019，257（11）：108735
[18] ALIPOUR M， SAHARKHIZ M J. Phytotoxic activity and variation in essential oil content and composition of Rosemary （Rosmarinus afficinalis L.） during different phenological growth stages[J]. Biocatalysis & Agricultural Biotechnology，2016，7：271-278
[19] OLIVEIRA COSTA R，JOSÉ C M，GROMBONE-GUARATINI M T，SILVA MATOS D M. Chemical characterization and phytotoxicity of the essential oil from the invasive Hedychium coronarium on seeds of Brazilian riparian trees[J]. Flora-Morphology Distribution Functional Ecology of Plants，2019，257：151411
[20] CHENG X Y，HOU Y，REN G H，et al. Allelopathic effects of aqueous extracts from “black soil patch” poisonous weeds on Elymus nutans in degraded alpine meadow[J]. Acta Botanica Boreali-Occidentalia Sinica，2011，31（10）：2057-2064 程晓月，后源，任国华，等. “黑土滩”退化高寒草地6种常见毒杂草水浸液对垂穗披碱草的化感作用[J]. 西北植物学报，2011，31（10）：2057-2064
[21] LIANG J. Allelopathic potential of major poisonous weeds and grasses in alpine meadows[D]. Xining：Qinghai University，2020：49-53 梁军. 高寒草甸主要毒杂草和禾草的化感潜力研究[D]. 西宁：青海大学，2020：49-53
[22] ZHANG Y Q，LI J M，CUI B H，et al. Research progress on the mechanism and application of rhizosphere microorganisms to the growth，development，and stress resistance of tobacco[J]. Biological Disaster Science，2024，47（4）：500-511 张玉芹，李军民，崔丙慧，等. 根际微生物对烟草生长发育及抗逆影响机制与应用研究进展[J]. 生物灾害科学，2024，47（4）：500-511
[23] ROSSMANN M，SARANGO-FLORES S W，CHIARAMONTE J B，et al. Plant microbiome： composition and functions in plant compartments[M]. Berlin：Springer，2017：7-20
[24] XU X R，DONG Y，FU W G. Effect of phenolic acid allelochemicals on nutrient supply capacity of rhizosphere soil[J]. Journal of Ecology and Rural Environment，2024，40（10）：1358-1365 徐香茹，董郁，付为国. 酚酸类化感物质对根际土壤养分供应能力的影响[J]. 生态与农村环境学报， 2024，40（10）：1358-1365
[25] ZHANG C Y，ZHAO Q，LIU H，et al. Research advances in microbial degradation of phenolic acids with allelopathic effects[J]. Microbiology China，2024，51（2）：402-418 张春杨，赵倩，刘红，等. 微生物降解植物化感物质酚酸的研究进展[J]. 微生物学通报，2024，51（2）：402-418
[26] KAUR H， KAUR R， KAUR S，et al. Taking ecological function seriously： soil microbial communities can obviate allelopathic effects of released metabolites[J]. Plos One，2009，4（3）：e4700
[27] LU F L. The allelopathy of Eucalyptus grandis x E.urophylla leaves and its relationship with soil microbes[D]. Guangzhou：South China Agricultural University，2017：27-41 卢凤来. 巨尾桉叶化感作用及其与土壤微生物的关系[D]. 广州：华南农业大学，2017：27-41
[28] CHEN M C，QIAO Y M，QUAN X L，et al. Physiological， biochemical and phytohormone responses of Elymus nutans to α-pinene-induced allelopathy[J]. PeerJ，2022，10：e14100
[29] JIANG O Y，LI L Y，DUAN G L，et al. Root exudates increased arsenic mobility and altered microbial community in paddy soils[J]. Journal of Environmental Sciences，2023，127（5）：410-420
[30] DING H Y. The allelopathic effect of garlic root exudate on different vegetables and their soil chemical and biological properties[D]. Yangling：Northwest A & F University，2017：92 丁海燕. 大蒜根系分泌物对不同受体蔬菜及其土壤的化感效应[D]. 杨凌：西北农林科技大学，2017：92
[31] LIU S R. Study on the formation mechanism and alleviation ways of continuous cropping obstacles of Pinellia ternata[D]. Chengdu：Chengdu University of TCM，2021：7-8 刘诗蓉. 半夏连作障碍的形成机理及缓解途径研究[D]. 成都：成都中医药大学，2021：7-8
[32] KONOPKA A，OLIVER L，TURCO R F. The use of carbon substrate utilization patterns in environmental and ecological microbiology[J]. Microbial Ecology，1998，35（2）：103-115
[33] MA Y S，JIE H D，TANG Y Y，et al. The role of hemicellulose in cadmium tolerance in ramie （Boehmeria nivea （L.） Gaud.）[J]. Plants，2022，11（15）：1941
[34] QU T B，DU X，PENG Y L，et al. Invasive species allelopathy decreases plant growth and soil microbial activity[J]. Plos One，2021，16（2）：e0246685
[35] ZHANG Q X，CHEN X T，WANG Y C，et al. Autotoxicity of continuously cropped passion fruit （Passiflora edulis Sims） Soil[J]. Allelopathy Journal，2021，53（1）：101-110
[36] SHI K， SHAO H. Changes in the soil fungal community mediated by a Peganum harmala allelochemical[J]. Frontiers in Microbiology，2022，13：911836
[37] ZHANG S Y，CUI B W，WANG J L，et al. Research progress on physiological and molecular responses of plant roots under abiotic stress[J]. Acta Agriculturae Zhejiangensis，2024，36（10）：2391-2401 张思懿，崔博文，王佳玲，等. 非生物胁迫下植物根系的生理与分子响应研究进展[J]. 浙江农业学报，2024，36（10）：2391-2401
[38] XIANG W，WEI X L，TANG H，et al. Complete genome sequence and biodegradation characteristics of benzoic acid-degrading bacterium Pseudomonas sp. SCB32[J]. Biomed Research International，2020，2020：6146104

Effects of Allelopathic α-Pinene on Feedback Interactions of Elymus Nutans and Soil

化感物质α-蒎烯对垂穗披碱草-土壤反馈作用的影响

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