Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (2): 581-591.DOI: 10.11733/j.issn.1007-0435.2026.02.020

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Comprehensive Evaluation of Seedling Drought Tolerance of 20 Medicago falcata Germplasm Resources

XIE Hong-yu, REN Qiu-yu, HE Qing-yun, LIU Chun, WANG Lin, LI Qian   

  1. 1. College of Grassland Science, Xinjiang Agricultural University, Urumqi, Xinjiang 830052, China
  • Received:2025-03-25 Revised:2025-05-06 Published:2026-01-22

20份黄花苜蓿种质资源苗期抗旱性综合评价研究

谢鸿宇, 任秋羽, 何清云, 刘纯, 王琳, 李倩   

  1. 1. 新疆农业大学草业学院, 新疆 乌鲁木齐 830052
  • 通讯作者: 李倩,E-mail:540794898@qq.com
  • 作者简介:谢鸿宇(2005-),女,汉族,四川绵阳人,本科生,主要从事牧草遗传育种研究,E-mail:2872291438@qq.com;
  • 基金资助:
    新疆维吾尔自治区重点研发项目(2023B02031);新疆维吾尔自治区牧草及草坪草种质资源圃建设项目资助

Abstract: In order to screen out the germplasm resources of Medicago falcata L. with strong drought tolerance, 20 resources of M. falcata were selected in this study, and their drought tolerance was comprehensively evaluated by analyzing the changes in the content of morphological and physiological indexes of the seedlings, and by adopting the affiliation function method and cluster analysis.The results showed that under different drought stress treatments, all the 20 M. falcata germplasm resources showed different drought responses, as indicated by a downward trend in plant height, leaf area, survival rate, above ground biomass and chlorophyll fluorescence parameters. The drought resistance of the 20 M. falcata seedlings was classified into 7 categories by cluster analysis, among which, the material A2Z311 had extremely drought-resistant, and the material A2Z525 had the weakest drought-resistant. Based on the D value of the affiliation function, the drought resistance was ranked from strong to weak as A2Z311>A2Z588>A2Z355>A2Z600>A2Z317>A2Z480>A2Z423>A2Z534>A2Z528>A2Z316>A2Z350>A2Z436>A2Z302>A2Z491>A2Z351>A2Z594>A2Z599>A2Z622>A2Z353>A2Z525. The present study indicates that the material A2Z311 could better adapt to the treatment of drought stress, and the survival rate, leaf area and chlorophyll fluorescence parameters all being 1, showing stronger drought resistance, which provides a theoretical basis for the selection and breeding of alfalfa varieties in arid areas.

Key words: Drought stress, Medicago falcata, Drought resistance

摘要: 为筛选出具有较强耐旱性能的黄花苜蓿(Medicago falcata L.)种质资源,本研究选用20份黄花苜蓿为研究对象,通过分析幼苗的形态和生理指标的含量变化,采用隶属函数法和聚类分析综合评价了20份黄花苜蓿的抗旱性。结果表明:在不同干旱胁迫处理下,20份黄花苜蓿种质均表现出不同的抗旱响应,表现为株高、叶面积、存活率、地上生物量和叶绿素荧光参数均呈下降趋势,聚类分析将20份黄花苜蓿幼苗的抗旱性分为7类,其中材料A2Z311抗旱性极强、材料A2Z525抗旱性极弱。基于隶属函数D值,其抗旱能力由强到弱排列为A2Z311>A2Z588>A2Z355>A2Z600>A2Z317>A2Z480>A2Z423>A2Z534>A2Z528>A2Z316>A2Z350>A2Z436>A2Z302>A2Z491>A2Z351>A2Z594>A2Z599>A2Z622>A2Z353>A2Z525。本研究表明,材料A2Z311能更好地适应干旱胁迫,存活率、叶面积及叶绿素荧光参数的隶属函数值均为1,表现出更强的抗旱性,这为干旱地区苜蓿品种的选育提供理论依据。

关键词: 干旱胁迫, 黄花苜蓿, 抗旱性