The Identification of the SWEET Gene Family in Oats and Its Expression Analysis in Response to Drought and Salt Stress

doi:10.11733/j.issn.1007-0435.2026.05.002

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (5): 1568-1583.DOI: 10.11733/j.issn.1007-0435.2026.05.002

The Identification of the SWEET Gene Family in Oats and Its Expression Analysis in Response to Drought and Salt Stress

LIU Jin-di^1,3, CHEN Shi-yong², LI Jin¹, LIANG Guo-ling¹, ZHOU Qing-ping²

1. Qinghai Academy of Animal Science and Veterinary Medicine, Qinghai University, Xining, Qinghai Province 810016, China;
2. Sichuan Zoige Alpine Wetland Ecosystem National Observation and Research Station, Southwest Minzu University, Chengdun, Sichuan Province 610041, China;
3. Institute of Tropical Eco-agricultural Yunnan Academy of Agricultural Sciences, Yuanmou, Yunnan Province 651300, China

Received:2025-08-20 Revised:2025-11-02 Published:2026-05-08

燕麦SWEET基因家族的鉴定及其响应干旱和盐碱胁迫的表达分析

刘进娣^1,3, 陈仕勇², 李进¹, 梁国玲¹, 周青平²

1. 青海大学畜牧兽医科学院, 青海西宁 810016;
2. 西南民族大学四川若尔盖高寒湿地生态系统国家野外科学观测研究站, 四川成都 610041;
3. 云南省农业科学院热区生态农业研究所, 云南元谋 651300

通讯作者: 陈仕勇，E-mail:chengshi8827@163.com
作者简介:刘进娣（1996-），女，汉族，甘肃白银人，博士研究生，主要从事种质分子方面研究，E-mail:liujindi6922@126.com；
基金资助:
四川省区域创新合作项目（2025YFHZ0282）；四川省“十四五”饲草育种攻关项目（2021YFYZ0013）和云南种子种业联合实验室元谋研发中心共享平台建设实验室项目（202405AR340001）资助

Abstract

Abstract: To identify the SWEET genes in Avena sativa and its responses to drought and salinity-alkalinity stress， this study systematically characterized the AsSWEET gene family using bioinformatics approaches and analyzed their expression patterns under drought and salt-alkali stress conditions by RT-qPCR. A total of 37 AsSWEET genes were identified and mapped to 19 chromosomes. All AsSWEET proteins contained the conserved MtN3_saliva domain， most members possessed seven transmembrane helices （TMHs）， and subcellular localization prediction indicated that they were primarily located on the plasma membrane. Phylogenetic analysis revealed that AsSWEET proteins could be classified into four major clades and shared high homology with SWEET proteins from Hordeum vulgare. Promoter analysis showed that AsSWEET family members harbored numerous cis-acting elements associated with light responsiveness， hormone signaling， development， and abiotic stress responses. Expression profiling indicated that 10， 7 and 18 AsSWEETs genes were significantly upregulated in leaves under drought， salt and alkali stress， respectively. Among them， AsSWEET15a and AsSWEET15c exhibited consistently high expression levels across all stress conditions， suggesting that they might serve as key candidate genes involved in oat tolerance to drought and salt-alkali stress. These findings provide theoretical insights into the functional roles of the AsSWEET gene family in abiotic stress responses and offer potential gene resources for molecular breeding of stress-tolerant oat varieties.

Key words: Avena sativa, SWEET proteins, Bioinformatics, Abiotic stress, Gene expression

摘要： 为鉴定燕麦（Avena sativa）基因组中SWEET基因家族成员，并揭示其在响应干旱和盐碱胁迫中的作用。本研究采用生物信息学方法对燕麦AsSWEET基因家族进行系统鉴定，同时基于RT-qPCR分析其在干旱和盐碱胁迫下的表达模式。结果表明，燕麦AsSWEET基因家族共有37个成员，分布于19条染色体上。AsSWEET蛋白均属于MtN3_saliva结构域，多数成员具有7个TMHs，亚细胞定位预测结果显示AsSWEET蛋白均位于质膜上。AsSWEET蛋白在进化关系上可分为4个分枝，且与大麦（Hordeum vulgare）SWEET蛋白具有较高的同源性。AsSWEET家族成员启动子区含大量的光响应、激素响应、生长发育及非生物胁迫相关的调控元件。表达模式分析得出，在干旱、盐和碱胁迫下，燕麦叶片中分别有10、7和18个AsSWEETs基因表达显著上调。其中AsSWEET15c和AsSWEET15a在各类胁迫中均呈现出较高的相对表达量，可作为燕麦响应干旱和盐碱胁迫的候选基因。本研究结果为深入解析燕麦AsSWEET基因家族在响应环境胁迫应答中的功能提供了参考。

关键词: 燕麦, SWEET蛋白, 生物信息学, 逆境胁迫, 基因表达

CLC Number:

S543+.7

LIU Jin-di, CHEN Shi-yong, LI Jin, LIANG Guo-ling, ZHOU Qing-ping. The Identification of the SWEET Gene Family in Oats and Its Expression Analysis in Response to Drought and Salt Stress[J]. Acta Agrestia Sinica, 2026, 34(5): 1568-1583.

刘进娣, 陈仕勇, 李进, 梁国玲, 周青平. 燕麦SWEET基因家族的鉴定及其响应干旱和盐碱胁迫的表达分析[J]. 草地学报, 2026, 34(5): 1568-1583.

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The Identification of the SWEET Gene Family in Oats and Its Expression Analysis in Response to Drought and Salt Stress

燕麦SWEET基因家族的鉴定及其响应干旱和盐碱胁迫的表达分析

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