Effect of Soda-Alkali Stress on Seed Germination and Seedling Growth of Pennisetum glaucum

doi:10.11733/j.issn.1007-0435.2026.05.015

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (5): 1737-1745.DOI: 10.11733/j.issn.1007-0435.2026.05.015

Effect of Soda-Alkali Stress on Seed Germination and Seedling Growth of Pennisetum glaucum

FENG Yu-ting¹, LUO Li-xuan¹, XU Qian¹, LU Rui¹, HU Long-xing^1,2

1. College of Agronomy, Hunan Agricultural University, Changsha, Hunan Province 410128, China;
2. Academician Workstation of Agricultural High-tech Industrial Area of the Yellow River Delta, National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Dongying, Shandong Province 257300, China

Received:2025-05-26 Revised:2025-08-25 Published:2026-05-08

苏打碱胁迫对美洲狼尾草种子萌发和幼苗生长的影响

奉雨婷¹, 罗李旋¹, 徐倩¹, 卢蕊¹, 胡龙兴^1,2

1. 湖南农业大学农学院, 湖南长沙 410128;
2. 黄三角农高区院士工作站/国家盐碱地综合利用技术创新中心, 山东东营 257300

通讯作者: 胡龙兴，E-mail:grass@hunau.edu.cn
作者简介:奉雨婷（2003-），女，汉族，湖南永州人，本科生，主要从事狼尾草逆境生物学相关的研究；
基金资助:
湖南省重点研发计划（2024WK2013）；黄三角国家农高区省级科技创新发展专项资金（2022SZX13）资助

Abstract

Abstract: To investigate the effects of soda-alkali stress at different concentrations on seed germination and seedling growth of pearl millet （Pennisetum glaucum）， twelve genotypes of pearl millet germplasms were used as experimental materials. Using the Petri dish filter paper germination method， soda-alkali stress was simulated by preparing mixed solutions of NaHCO₃ and Na₂CO₃ at a molar ratio of 9∶1， with concentration gradients of 0， 40， and 60 mmol·L^-1. Germination-related parameters， encompassing germination rate， germination potential， germination index， vigor index， radicle emergence length， and plumule development length， were measured during the seed imbibition phase. A comprehensive evaluation framework for assessing soda-alkali stress tolerance was established through fuzzy membership function analysis combined with principal component clustering methodology. The results demonstrated that soda-alkali stress significantly inhibited seed germination and seedling growth of pearl millet， with the inhibitory effects intensified as the concentration of soda-alkali solution increased. Furthermore， substantial genotypic variation was observed in soda-alkali stress tolerance during the germination stage among different pearl millet genotypes. Cluster analysis classified the 12 genotypes into four distinct categories：（1） soda-alkali tolerant type （represented by Ca1165， accounting for 8.33%），（2） moderately tolerant type （including Ca399， Ca727， Ca396， Ca742， and Ca382， comprising 41.67%），（3） sensitive type （comprising Ca425， Ca481， Ca736， and Ca406， representing 33.33%）， and （4） highly sensitive type （consisting of Ca424 and Ca397， accounting for 16.67%）.

Key words: Soda-alkali stress, Pennisetum glaucum L., Seed germination, Seedling growth

摘要： 为明确不同浓度苏打碱胁迫对美洲狼尾草（Pennisetum glaucum）种子萌发和幼苗生长的影响，以期筛选出耐苏打碱性强的美洲狼尾草品种。本研究选用12份不同基因型美洲狼尾草种质为试验材料，采用培养皿纸上发芽法，将NaHCO₃和Na₂CO₃按9∶1摩尔比例混合后配置浓度梯度为0，40，60 mmol·L^-1的苏打碱溶液模拟不同程度的苏打碱胁迫，测定美洲狼尾草种子萌发期的发芽率、发芽势、发芽指数、活力指数、胚根长和胚芽长，并采用隶属函数和聚类分析对12份美洲狼尾草的耐苏打碱能力进行综合分析。结果表明，苏打碱胁迫显著抑制美洲狼尾草种子萌发和幼苗生长，且随着苏打碱溶液浓度升高而加重。不同基因型美洲狼尾草种子萌发期的耐苏打碱胁迫能力存在显著差异，通过聚类分析可将12份美洲狼尾草分为四类，分别为耐苏打碱型（Ca1165，占比8.33%）、中等耐苏打碱型（Ca399，Ca727，Ca396，Ca742，Ca382，占比41.67%）、敏感型（Ca425，Ca481，Ca736，Ca406，占比33.33%）和高敏感型（Ca424，Ca397，占比16.67%）。

关键词: 苏打碱胁迫, 美洲狼尾草, 种子萌发, 幼苗生长

CLC Number:

S544.9

FENG Yu-ting, LUO Li-xuan, XU Qian, LU Rui, HU Long-xing. Effect of Soda-Alkali Stress on Seed Germination and Seedling Growth of Pennisetum glaucum[J]. Acta Agrestia Sinica, 2026, 34(5): 1737-1745.

奉雨婷, 罗李旋, 徐倩, 卢蕊, 胡龙兴. 苏打碱胁迫对美洲狼尾草种子萌发和幼苗生长的影响[J]. 草地学报, 2026, 34(5): 1737-1745.

References

[1] YANG C W, LI C Y, YIN H J, et al. Physiological response of xiaobingmai (Triticum aestivum-Agropyron intermedium) to salt-stress and alkali-stress[J]. Acta Agronomica Sinica, 2007, 33(8):1255-1261 杨春武, 李长有, 尹红娟, 等. 小冰麦(Triticum aestivum-Agropyron intermedium)对盐胁迫和碱胁迫的生理响应[J]. 作物学报, 2007, 33(8):1255-1261
[2] YUE J M, REN Q, ZHANG J C. Research progress on plant salt tolerance mechanism[J]. China Forestry Science and Technology, 2015, 29(5):9-13 岳健敏, 任琼, 张金池. 植物盐耐机理研究进展[J]. 林业科技开发, 2015, 29(5):9-13
[3] WEI L X. Priming effect and the mechanism of abscisic acid on saline-alkaline stress tolerance in rice(Oryza Sativa L.)[D]. Changchun:Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, 2015:6 魏立兴. 外源脱落酸对水稻(Oryza sativa L.)盐碱胁迫的诱抗效应及其生理机制[D]. 长春:中国科学院东北地理与农业生态研究所, 2015:6
[4] LIU J X, WANG J C, JIA H Y. Differences between physiological responses of avena nuda seedlings to salt and alkali stresses [J]. Journal of Soil and Water Conservation, 2015, 29(5):331-336 刘建新, 王金成, 贾海燕. 燕麦幼苗对盐胁迫和碱胁迫的生理响应差异[J]. 水土保持学报, 2015, 29(5):331-336
[5] YANG C W, LI C Y, ZHANG M L, et al. pH and ion balance in wheat-wheatgrass under salt-or alkali stress[J]. Chinese Journal of Applied Ecology, 2008, (5):1000-1005 杨春武, 李长有, 张美丽, 等. 盐、碱胁迫下小冰麦体内的pH及离子平衡[J]. 应用生态学报, 2008, (5):1000-1005
[6] YU C, ZHAGN Y L, LI Q Y, et al. Effects of saline-alkali stresses on seed germination and seedling physiological and biochemical characteristic[J]. Acta Agrestia Sinica, 2024, 32(6):1882-1892 于婵, 张依琳, 李秋莹, 等. 盐碱胁迫对牛至种子萌发和幼苗生理生化特性的影响[J]. 草地学报, 2024, 32(6):1882-1892
[7] WU Y, TIAN Y, ZHANG H X, et al.Effects of salinity, alkalinity, temperature and their interactions on seed germination of Medicago falcata[J]. Pratacultural Science, 2015, 32(11):1847-1853 武祎, 田雨, 张红香, 等. 盐、碱胁迫与温度对黄花苜蓿种子发芽的影响[J]. 草业科学, 2015, 32(11):1847-1853
[8] LI L, WU H Q, MA C Y, et al. Growth promotion and salt tolerance induction by Piriformospora indica colonization in Medicago truncatula[J]. Microbiological Bulletin, 2015, 42(8):1492-1500 李亮, 武洪庆, 马朝阳, 等. 印度梨形孢促进蒺藜苜蓿生长及其提高耐盐性研究[J]. 微生物学通报, 2015, 42(8):1492-1500
[9] WU Z Z, ZHANG X L, MA M. Effects of licorice planting on physical, chemical properties and microbial function diversity of saline soil[J]. Journal of Agriculture, 2016, 6(7):24-29 吴振振, 张旭龙, 马淼. 栽培甘草对盐碱地土壤理化性质及微生物功能多样性的影响[J]. 农学学报, 2016, 6(7):24-29
[10] ZHAGN T T, LIU Y L, CHEN H, et al. Effects of different exogenous substances on the seed germination, seedling growth, and physiology of Melilotus suaveolens under salt, alkali, and drought stress [J]. Acta Prataculturae Sinica, 2024, 33(8):122-132 张婷婷, 刘宇乐, 陈红, 等. 不同外源物质对盐、碱及干旱胁迫下草木樨种子萌发、幼苗生长及生理的影响[J]. 草业学报, 2024, 33(8):122-132
[11] YU W C, ZHU Y H, XU Q, et al. Effect of cadmium stress on seed germination and growth of Amaranthus cruentus germplasms from different locations[J]. Pratacultural Science, 2022, 39(9):1815-1822 喻望晨, 朱依晗, 徐倩, 等. 镉胁迫对不同繁穗苋种质萌发和生长的影响[J]. 草业科学, 2022, 39(9):1815-1822
[12] Gulineier·Yasen, YANG R R, ZENG Y L. Effects of salt-alkali mixed stresses on seed germination of the halophyte Chenopodium glaucum L.[J]. Chinese Journal of Ecology, 2014, 33(1):76-82 古丽内尔·亚森, 杨瑞瑞, 曾幼玲. 混合盐碱胁迫对灰绿藜(Chenopodium glaucum L.)种子萌发的影响[J]. 生态学杂志, 2014, 33(1):76-82
[13] SHEN Y. Effect of saline alkli environmental stresses on sod antioxidant enzymes in maize seeds during germination[J]. Journal of Green Science and Technology, 2022, 24(13):121-122, 126 沈彦. 盐碱环境胁迫对吸胀萌发期玉米种子SOD活性的影响[J]. 绿色科技, 2022, 24(13):121-122, 126
[14] LI B, CHEN X M, YU H L, et al. Effects of mixed saline-alkali stress on germination characteristics of alfalfa seeds[J]. Jiangsu Agricultural Sciences, 2015, 43(8):221-225 李波, 陈雪梅, 于海龙, 等. 混合盐碱胁迫对苜蓿种子萌发特性的影响[J]. 江苏农业科学, 2015, 43(8):221-225
[15] CHEN Z L, ZHANG X Y, ZHAGN M, et al. Effects of alkaline stress on seeds germination and embryo growth of zoysia japonica and festuca arundinacea[J]. Seed, 2010, 29(12):27-30 陈忠林, 张学勇, 张绵, 等. 碱胁迫对结缕草、高羊茅种子萌发及其胚生长的影响[J]. 种子, 2010, 29(12):27-30
[16] DAI L Y, ZHANG L J, ZHANG C C. Effect of saline-sodic stress on the sweet sorghum germination and evaluation of salt tolerance on the different varieties[J]. Seed, 2011, 30(10):28-32 戴凌燕, 张立军, 张成才. 苏打盐碱胁迫对甜高粱种子萌发的影响及品种耐性综合评价[J]. 种子, 2011, 30(10):28-32
[17] WANG Z, YOU J, XU X, et al. Physiological and biochemical responses of Melilotus albus to saline and alkaline stresses[J]. Horticulturae, 2024, 10(3):297
[18] Gao Z W, Mu Y G, Di J J, et al. The effects of salinity stress on Amorpha fruticosa L. seed germination, physiological and biochemical mechanisms[J]. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 2024, 52(1):13552-13552
[19] NIE T Y, ZHU Y H, LIU X F, et al. Comprehensive evaluation of cadmium tolerance at germination stage of 16 amaranthus caudatus germplasms[J]. Chinese Journal of Grassland, 2022, 44(5):66-72 聂婷宇, 朱依晗, 刘霄霏, 等. 16份尾穗苋种质萌发期耐镉性综合评价[J]. 中国草地学报, 2022, 44(5):66-72
[20] CHEN X, ZHANG Z W, WU B. Comprehensive evaluation of salt tolerance and screening for salt tolerant accessions of naked oat (Avena nuda L.) at germination stage[J]. Scientia Agricultura Sinica, 2014, 47(10):2038-2046 陈新, 张宗文, 吴斌. 裸燕麦萌发期耐盐性综合评价与耐盐种质筛选[J]. 中国农业科学, 2014, 47(10):2038-2046
[21] CHEN Z T, HE S L, HUAN Y B. Research progress of pennisetum rich.[J]. Acta Agrestia Sinica, 2010, 18(5):740-748 陈志彤, 何水林, 黄毅斌. 狼尾草属牧草研究进展[J]. 草地学报, 2010, 18(5):740-748
[22] WANG J J, Abudushalamu, ZHU S C, et al. Genome-wide identification and analysis of the carotenoid cleavage dioxygenase(ccd) gene family in Pennisetum glaucum[J]. Chinese Journal of Grassland, 2024, 46(3):11-21 王净洁, 阿布都沙拉木, 祝诗词, 等. 美洲狼尾草CCD基因家族全基因组鉴定及分析[J]. 中国草地学报, 2024, 46(3):11-21
[23] ZHONG X X, BAI S J, GU H R, et al. RAPD analysis for male-sterile line, R-line and F1 hybrid of Pennisetum americanum[J]. Acta Prataculturae Sinica, 2001, 10(3):28-32 钟小仙, 白淑娟, 顾洪如, 等. 美洲狼尾草不育系、恢复系及杂种F₁的RAPD分析[J]. 草业学报, 2001, 10(3):28-32
[24] FAN Y H, XU Y, ZHU S C, et al. Construction of tissue culture regeneration system of Pennisetum glaucum[J]. Acta Agrestia Sinica, 2023, 31(1):89-95 范欲航, 许悦, 祝诗词, 等. 美洲狼尾草组织培养再生体系的建立[J]. 草地学报, 2023, 31(1):89-95
[25] ZHONG X X, GU H R, ZHOU W X, et al. Cluster analysis and comprehensive evaluation on genetic diversity of germplasm resources in Pennisetum americanum [J]. Jiangsu Agricultural Sciences, 2001(5):63-66 钟小仙, 顾洪如, 周卫星, 等. 美洲狼尾草种质资源遗传多样性聚类分析与综合评价[J]. 江苏农业科学, 2001(5):63-66
[26] QIN G Q, CHEN, SU D H. Effects of salt stress on seed germination and seedling growth of 4 graminaceous pasture cultivars[J]. Chinese Agricultural Science Bulletin, 2005, 21(3):205-206, 232 覃广泉, 陈平, 苏东海. 盐胁迫对4种禾本科牧草种子萌发和幼苗生长的影响[J]. 中国农学通报, 2005, 21(3):205-206, 232
[27] WU F P, HAN Q F, JIA Z K. Preliminary study on the salt tolerance of four alfalfa lines in seed germination period[J]. Pratacultural Science, 2008, 25(8):57-62 吴凤萍, 韩清芳, 贾志宽. 4个白花苜蓿品系种子萌发期耐盐性研究[J]. 草业科学, 2008, 25(8):57-62
[28] JIA X F. Effect of soda salt-alkaline stress on seed germination and salt tolerance evaluation analysis of two Medicago sativa varieties [J]. Acta Agrestia Sinica, 2019, 27(6):1511-1517 贾秀峰. 苏打盐碱胁迫对两种苜蓿种子萌发影响及品种耐性综合分析[J]. 草地学报, 2019, 27(6):1511-1517
[29] LIN J X, GAO Z W, WANG Y, et al. Synergistic effects of salt and alkaline stresses on the seed germination of Medicago sativa[J]. Acta Agrestia Sinica, 2014, 22(2):312-318 蔺吉祥, 高战武, 王颖, 等. 盐碱胁迫对紫花苜蓿种子发芽的协同影响[J]. 草地学报, 2014, 22(2):312-318
[30] WANG B, SONG F B. Physiological responses and adaptive capacity of oats to saline-alkali stress[J]. Ecology and Environment, 2006, 15(3):625-629 王波, 宋凤斌. 燕麦对盐碱胁迫的反应和适应性[J]. 生态环境, 2006, 15(3):625-629
[31] WANG T X. Differential effects of saline and alkaline stress on water uptake and germination characteristics of alfalfa seed germination[EB/OL]. http://kns.cnki.net/kcms/detail/62.1069.S.20250313.1326.008.html, 2025-03-13/2025-04-10 王跳霞. 盐碱胁迫对紫花苜蓿种子萌发吸水及萌发特性影响的差异性[EB/OL].http://kns.cnki.net/kcms/detail/62.1069.S.20250313.1326.008.html, 2025-03-13/2025-04-10
[32] LIN J X, LI X Y, TANG J H, et al. Effects of salt and alkali stresses on seed germination, early seedling growth and the metabolize of Na⁺ and K⁺ in shoots of wheat[J]. Journal of Triticeae Crops, 2011, 31(6):1148-1152 蔺吉祥, 李晓宇, 唐佳红, 等. 盐碱胁迫对小麦种子萌发、早期幼苗生长及Na+、K+代谢的影响[J].麦类作物学报, 2011, 31(6):1148-1152
[33] ZHANG J D, WANG C, LU H, et al. Response of mung bean genotypes to exogenous brassinosteroids under saline-alkali stress[J]. Crops, 2025, (5):54-60 张金东, 王成, 卢环, 等. 盐碱胁迫下不同基因型绿豆对外源BR的响应[J]. 作物杂志, 2025, (5):54-60

Effect of Soda-Alkali Stress on Seed Germination and Seedling Growth of Pennisetum glaucum

苏打碱胁迫对美洲狼尾草种子萌发和幼苗生长的影响

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	YANG Jin-hui, WANG Xiao-tong, MA Yong-long, YU Dong-wen, TONG Yu-hua, MA Meng-fan, LI Shu-xia. Evaluation of Salt Tolerance of Different Alfalfa Germplasm Materials During the Germination Period [J]. Acta Agrestia Sinica, 2026, 34(5): 1654-1663.
[2]	SU Li-he, HUANG Rong-zheng, HU Tian-yu, MA Chun-hui. Comprehensive Evaluation of Salt Tolerance of different Alfalfa （Medicago sativa L.） Cultivars during Germination and Seedling Stages under Salt Stress [J]. Acta Agrestia Sinica, 2026, 34(5): 1664-1676.
[3]	HUANG Meng-qi, LI Chen-jian, WANG Yu-xiang, LU Lu, WANG Qing-yu, JIANG Yiu, TONG Ai-xiang. Comprehensive Evaluation of Drought Tolerance in Bromus inermis Germplasm at the Germination Stage [J]. Acta Agrestia Sinica, 2026, 34(5): 1677-1690.
[4]	GE Meng-ting, QIAO Dan-dan, WANG Xin-yue, ZHANG Ruo-lin, BI Mingyue, HE Xue-qing. Effects of Cerium Oxide Nanoparticle Priming on Seed Germination and Physiological Characteristics of Alfalfa under Drought Stress [J]. Acta Agrestia Sinica, 2026, 34(4): 1534-1545.
[5]	HU Er-ai, ZHENG Kai-xin, JI Jia-hui, XIN Bing, LIU Ling-yun, TENG Ke, FAN Xi-feng, YUE Yue-sen, DING Guo-chang. Physiological Response and Evaluation of Three Phalaris arundinacea Resources to Low-Dose ⁶⁰Co-γ Radiation-Induced Mutagenesis [J]. Acta Agrestia Sinica, 2026, 34(1): 132-141.
[6]	PI Dong-ge, LI Hai-yan, YANG Yun-fei, GUO Jian, YUE Xiu-quan, LI Ya-nan. Effects of Saline-alkali Stress on Seed Germination of Artemisia sieversiana and A. lavandulifolia under Different Temperatures [J]. Acta Agrestia Sinica, 2026, 34(1): 231-238.
[7]	GU Tao, BAO Sai-hen-na, MIAO Yan-jun, HAN You-jian, SU Zhi-gang, ZHUO Ma-yong-qing. Study on the Adaptability of Seed Germination and Physiology of Three Grasses to Diurnal Temperature Change in Alpine Region [J]. Acta Agrestia Sinica, 2026, 34(1): 266-274.
[8]	MENG Qing-xian, LUO Qin, ZHAO Zhi-li, DUAN Xin-hui, HAN Bo. Responses of Seed Germination and Seedling Growth of Three Asteraceae Weeds to Simulated Nitrogen Deposition [J]. Acta Agrestia Sinica, 2025, 33(9): 2832-2842.
[9]	JI Ling-he, HE Ao-lei, HE Feng, LI Chang-ning, YAO Tuo. Screening of Alfalfa Seed Coating Materials and Theirs Effect on Seed Germination [J]. Acta Agrestia Sinica, 2025, 33(8): 2728-2736.
[10]	HAN Zhan-jiang, ZHAO Qing-qing, JIANG Li. Study on Salt Tolerance Characteristics of Medicinal Plants Apocynum venetum and Apocynum pictum [J]. Acta Agrestia Sinica, 2025, 33(6): 1800-1806.
[11]	WANG Hui-zhi, LIAO Zhi-min, ZHANG Zhe, XIE Wen-gang, WANG Zhao-ming, LIU Zhi-peng. Effects of EMS Treatment on Germination and the Physiological and Biochemical Indicators of Elymus sibiricus [J]. Acta Agrestia Sinica, 2025, 33(6): 1834-1842.
[12]	LYU Yan-zhen, YAO Xing-jie, ZHANG Zhao, SUN Qing-ying, YANG Yu-ze, YAN Hui-fang. Evaluation of Alkali Tolerance and Key Indicators Screening during Seed Germination of Different Alfalfa Cultivars [J]. Acta Agrestia Sinica, 2025, 33(6): 1852-1861.
[13]	WANG Yi-ting, LIU Yu, HAN Yue, NIAN Ren-jie, JIANG Heng-yue, CUI Ying, YANG Zhi-min, ZHANG Xia-xiang. Effects of Simulated Drought and Salt Stresses on Seed Germination and Seeding Growth of Pink Muhly Grass [J]. Acta Agrestia Sinica, 2025, 33(5): 1465-1472.
[14]	YAO Xing-jie, SUN Qing-ying, ZHANG Zhao, LYU Yan-zhen, YAN Si-qi, CHEN Si-yu, SHI Yan-xin, DUAN Xiao-tong, MENG Shu-han, YAN Hui-fang. Comprehensive Evaluation on Salt Tolerance of 61 Alfalfa Cultivars during Seed Germination [J]. Acta Agrestia Sinica, 2025, 33(4): 1181-1191.
[15]	LI Chang-ran, LIU Jing-yu, LI Ruo-hong, MAO Pei-sheng. Effects of Biochar Encrusting on Drought Resistance Characteristics of Agropyron Mongolicum during Seed Germination [J]. Acta Agrestia Sinica, 2025, 33(3): 960-967.