Study on the Adaptability of Seed Germination and Physiology of Three Grasses to Diurnal Temperature Change in Alpine Region

doi:10.11733/j.issn.1007-0435.2026.01.025

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (1): 266-274.DOI: 10.11733/j.issn.1007-0435.2026.01.025

Study on the Adaptability of Seed Germination and Physiology of Three Grasses to Diurnal Temperature Change in Alpine Region

GU Tao, BAO Sai-hen-na, MIAO Yan-jun, HAN You-jian, SU Zhi-gang, ZHUO Ma-yong-qing

Xizang Agriculture and Animal Husbandry University, Nyingchi, Xizang 860000, China

Received:2025-02-20 Revised:2025-04-14 Published:2025-12-24

3种禾本科牧草种子萌发及生理对高寒昼夜变温的适应性研究

顾涛, 包赛很那, 苗彦军, 韩有健, 苏志刚, 卓玛永青

西藏农牧大学, 西藏林芝 860000

通讯作者: 包赛很那,E-mail:1306598067@qq.com
作者简介:顾涛（2001-），男，汉族，陕西宝鸡人，硕士研究生，主要从事野生牧草种质资源选育相关研究，E-mail:3197099541@qq.com；
基金资助:
西藏农牧学院研究生创新计划资助项目（YJS2025-17）；中国农业大学-西藏农牧学院科研联合基金项目（2024TC082）；国家自然科学基金项目，EnCOR401基因调控垂穗披碱草耐低温机制研究（32260342）；中央引导地方科技发展资金项目，巴青垂穗披碱草种子繁育生产体系研究与示范（ZYYD2023000147）资助

Abstract

Abstract: This study investigated the temperature adaptability of three grass species （Elymus nutans， Bromus inermis， and Festuca sinensis） through the controlled experiments simulating diurnal thermal regimes （10℃/5℃， 15℃/5℃， 20℃/5℃， 25℃/10℃） characteristic of their natural habitat during March-November. We systematically analyzed seed germination dynamics （quantified by germination rate， potential， and index）， seedling growth parameters， and physiological stress markers to elucidate species-adaptive responses to temperature gradients in Alpine region. With the increase of temperature， the germination rate， germination potential and germination index of the Elymu snutans seeds all increased first and then decreased. The germination rate， germination potential and germination index of Bromus inermis and Festuca sinensis seeds increased significantly with the increase of temperature. The contents of malondialdehyde （MDA）， soluble sugar （SS） and free proline （Pro） of the three grass seedlings increased with decreasing temperature. The results showed that the optimum temperature was 20℃/5℃ for Elymus nutans， 25℃/10℃ for Bromus inermis and Festuca sinensis. The comprehensive evaluation of the membership function showed that the temperature adaptability of the three grass species was Elymu snutans > Bromus inermis > Festuca sinensis.

Key words: Temperature change, Elymus nutans, Bromus inermis, Festucas inensis, Seed germination, Physiological characteristics

摘要： 本研究以3种禾本科牧草垂穗披碱草（Elymu snutans）、无芒雀麦（Bromus inermis）和中华羊茅（Festuca sinensis）作为供试材料，模拟高寒地区植物自然生境中的昼夜变温（3—11月）设置10℃/5℃、15℃/5℃ 、20℃/5℃和25℃/10℃等4种温度，进行种子萌发试验，测定种子萌发及苗期生理指标，阐明3 种禾本科牧草对高寒生境中温度变化的适应性。结果表明：随着温度的升高，垂穗披碱草种子发芽率、发芽势和发芽指数均呈先提高后下降的趋势；无芒雀麦和中华羊茅种子发芽率、发芽势和发芽指数均随温度的升高而显著提高。3种牧草幼苗丙二醛、可溶性含糖和游离脯氨酸含量均随着温度降低呈升高趋势。综上，垂穗披碱草最适温萌发度为20℃/5℃，无芒雀麦和中华羊茅最适萌发温度为25℃/10℃；隶属函数综合评价结果表明：3种牧草对高寒温度适应性由强至弱的顺序：垂穗披碱草> 无芒雀麦>中华羊茅。

关键词: 变温, 垂穗披碱草, 无芒雀麦, 中华羊茅, 种子萌发, 生理特性

CLC Number:

S543

GU Tao, BAO Sai-hen-na, MIAO Yan-jun, HAN You-jian, SU Zhi-gang, ZHUO Ma-yong-qing. Study on the Adaptability of Seed Germination and Physiology of Three Grasses to Diurnal Temperature Change in Alpine Region[J]. Acta Agrestia Sinica, 2026, 34(1): 266-274.

顾涛, 包赛很那, 苗彦军, 韩有健, 苏志刚, 卓玛永青. 3种禾本科牧草种子萌发及生理对高寒昼夜变温的适应性研究[J]. 草地学报, 2026, 34(1): 266-274.

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Study on the Adaptability of Seed Germination and Physiology of Three Grasses to Diurnal Temperature Change in Alpine Region

3种禾本科牧草种子萌发及生理对高寒昼夜变温的适应性研究

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