Acta Agrestia Sinica

›› 2008, Vol. 16 ›› Issue (4): 359-363.DOI: 10.11733/j.issn.1007-0435.2008.04.008

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Studies on Callus Induction and Plant Regeneration of Minshan Red Clover

HOU Yan-ming1,2,3, SHI Shang-li1,2,3, DU Wen-hua1,2,3,4, ZHOU Wan-hai1,2,3   

  1. 1. Pratacultural College, Gansu Agricultural University, Lanzhou, Gansu Province 730070, China;
    2. Key Laboratory of Grassland Ecosystem(Gansu Agricultural University), Ministry of Education, Lanzhou, Gansu Province 730070;
    3. Sino-US Center for Grazingland Ecosystem Sustainability, Lanzhou, Gansu Province 730070, China;
    4. Key Laboratory of Arid and Grassland Ecology(Lanzhou University), Ministry of Education, Lanzhou, Gansu Province 730000, China
  • Received:2007-10-29 Revised:2008-03-10 Online:2008-08-15 Published:2008-08-15

岷山红三叶愈伤组织诱导和植株再生研究

厚彦明1,2,3, 师尚礼1,2,3, 杜文华1,2,3,4, 周万海1,2,3   

  1. 1.甘肃农业大学草业学院, 兰州, 730070;
    2. 草业生态系统教育部重点实验室, 甘肃农业大学, 兰州, 730070;
    3. 中-美草地畜牧业可持续研究中心, 兰州, 730070;
    4. 干旱与草地生态教育部重点实验室, 兰州大学, 兰州, 730070
  • 通讯作者: 杜文华,E-mail:duwh@gsau.edu.cn
  • 作者简介:厚彦明(1982- ),甘肃甘谷人,硕士研究生,研究方向为牧草育种及种质资源,E-mail:ym-hou@163.com
  • 基金资助:
    草业生态系统教育部省部共建重点实验室项目(CY-GG-2006-17);中国博士后科学基金项目

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Abstract: Tissue culture technique is the basis for plant breeding and genetic biodiversity research.Through the study in plant regeneration of hypocotyl and cotyledon of Trifolium pratense L.cv.Minshan,we found that the optimal callus induction medium was MS+2 mg/L 2,4-D +0.5 mg/L 6-BA+2% sucrose+0.6% agar;the best medium for embryogenic callus induction was MS+0.1 mg/L IAA+0.5 mg/L 6-BA+2% sucrose+0.6% agar;bud induction medium was B5+0.06 mg/L NAA+2% sucrose+0.6%.agar.In each bottle,2 to 10 stems were obtained and then transferred into root induction medium,buds could continually grow.The optimal root induction medium was 1/2 MS+0.05 mg/L NAA+1.5% sucrose +0.8% agar.160 d was needed for the establishment of plantlet.

Key words: Trifolium pratense L.cv.Minshan, Callus, Plant regeneration

摘要： 通过对岷山红三叶下胚轴和子叶再生体系建立的研究,获得愈伤组织诱导的最佳培养基为:MS+2 mg/L 2,4-D+0.5 mg/L6-BA+2%蔗糖+0.6%琼脂,胚性愈伤组织诱导培养基为:MS+0.1 mg/L IAA+0.5 mg/L 6-BA+2%蔗糖+0.6%琼脂,芽诱导培养基为:B5+0.06 mg/L NAA+2%蔗糖+0.6%琼脂,每瓶可以长出2~10条茎,将茎转入生根培养基后愈伤继代可持续出芽。最佳的生根培养基为:1/2 MS+0.05mg/LNAA+1.5%蔗糖+0.8%琼脂。建立再生植株约需160 d。

关键词: 岷山红三叶, 愈伤组织, 植株再生

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