菌物学报 ›› 2019, Vol. 38 ›› Issue (11): 1976-1991.doi: 10.13346/j.mycosystema.190119

• 研究论文 • 上一篇    下一篇

保水剂和丛枝菌根真菌异形根孢囊霉对紫花苜蓿生长与抗旱性的影响

于萌1,2,张永帅1,3,付伟1,2,吴照祥1,4,谢伟1,2,张莘1,郝志鹏1,*(),陈保冬1,2   

  1. 1. 中国科学院生态环境研究中心 城市与区域生态国家重点实验室 北京 100085
    2. 中国科学院大学 北京 100049
    3. 河南科技学院食品学院 河南 新乡 453003
    4. 江西省科学院生物资源研究所 江西 南昌 330096
  • 收稿日期:2019-04-12 接受日期:2019-07-02 出版日期:2019-11-22 发布日期:2019-12-14
  • 通讯作者: 郝志鹏 E-mail:zphao@rcees.ac.cn
  • 作者简介:郝志鹏,博士,中国科学院生态环境研究中心城市与区域国家重点实验室助理研究员,从事微生物在土壤生态过程与生态重建中作用及其调控机制研究,重点关注菌根真菌提高植物抵抗病原生物胁迫的生理和生态机制,以及菌根生物技术原理与应用等方面。目前主持和参与多项国家科研项目,发表论文40余篇,参编学术专著2部,授权专利1项。
  • 基金资助:
    国家自然科学基金(41571250);国家重点研发计划(2016YFC0500702)

Effects of arbuscular mycorrhizal fungi Rhizophagus irregularis and super absorbent polymers on growth and drought tolerance of Medicago sativa

Meng YU1,2,Yong-Shuai ZHANG1,3,Wei FU1,2,Zhao-Xiang WU1,4,Wei XIE1,2,Xin ZHANG1,Zhi-Peng HAO1,*(),Bao-Dong CHEN1,2   

  1. 1. State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China
    2. University of Chinese Academy of Sciences, Beijing 100049, China
    3. College of Food Science, Henan Institute of Science and Technology, Xinxiang, Henan 453003, China
    4. Institute of Biological Resources, Jiangxi Academy of Sciences, Nanchang, Jiangxi 330096, China
  • Received:2019-04-12 Accepted:2019-07-02 Online:2019-11-22 Published:2019-12-14
  • Contact: Zhi-Peng HAO E-mail:zphao@rcees.ac.cn
  • Supported by:
    Supported by the National Natural Science Foundation of China(41571250);and National Key Research and Development Program of China(2016YFC0500702)

摘要:

为探讨保水剂(super absorbent polymers,SAP)和丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF)对植物生长和抗旱性的影响,以紫花苜蓿Medicago sativa为供试植物,开展了温室盆栽试验。植物播种时设置土壤添加和不添加聚丙烯酰胺型SAP(BJ2101)处理,以及接种和不接种异形根孢囊霉Rhizophagus irregularis处理,通过称重法维持12%的土壤含水量(正常供水),植物生长30d,各处理一半植物接受干旱胁迫(6%的土壤含水量),另一半仍正常供水,持续30d后收获。结果表明,在干旱胁迫下,接种AMF显著增加了紫花苜蓿的植株干重,促进了植物对矿质元素的吸收,提高了叶片中叶绿素和脯氨酸含量,增强了植物的抗旱性。SAP抑制了R. irregularis对植物根系的侵染;与单接种AMF相比,SAP和AMF的联合施用降低了紫花苜蓿的生物量,影响了植物对矿质元素的吸收。本研究中,SAP和AMF的联合施用并没有表现出协同增效作用,这一方面可能是因为研究设定的土壤水分管理模式,另一方面SAP与AMF共同施用的适宜条件还需进一步探索优化。

关键词: 丛枝菌根真菌, 保水剂, 紫花苜蓿, 植物生长, 抗旱性

Abstract:

To investigate the interactive effects of arbuscular mycorrhizal fungi (AMF) and super absorbent polymers (SAP) on Medicago sativa plant growth and drought resistance, a pot experiment was conducted under greenhouse conditions. Treatment with or without Rhizophagus irregularis and addition or without addition of polyacrylamide type SAP (BJ2101) to the soil were designed under well-watered (12% water content) or drought conditions (6% water content). Results showed that the inoculation with AMF significantly increased plant dry weight under drought stress, facilitated the uptake of mineral elements, and increased the leaf chlorophyll and proline concentration. However, SAP inhibited the root colonization of R. irregularis. Compared with single inoculation of AMF, SAP combined with AMF reduced plant biomass and negatively affected the absorption of mineral elements by plants under drought stress. The combined application of SAP and AMF did not show synergistic effects, and this might be due to the defective soil moisture management. The co-application of SAP and AMF required further optimization.

Key words: arbuscular mycorrhizal fungi (AMF), super absorbent polymers (SAP), Medicago sativa, plant growth, drought resistance