羊草幼穗离体培养诱导植株再生的研究

doi:10.11733/j.issn.1007-0435.2002.03.009

›› 2002, Vol. 10 ›› Issue (3): 198-202.DOI: 10.11733/j.issn.1007-0435.2002.03.009

羊草幼穗离体培养诱导植株再生的研究

刘公社, 汪恩华, 刘杰, 齐冬梅, 李芳芳

中国科学院植物研究所, 北京, 100093

收稿日期:2002-01-31 修回日期:2002-03-28 出版日期:2002-08-15 发布日期:2002-08-15
作者简介:刘公社(1958- ),男,陕西人,1986年在法国获生物学博士学位.1986-1988年回国从事博士后研究工作,现为中国科学院植物研究所首席研究员,研究方向为生物工程及其在持续农业上的应用.发表学术论文72篇,编著3部.
基金资助:
Supported by Key Project of the Chinese Academy of Science (KSCX1-08-03) and State Key Project of the Ministry of Science and Technology (2001BA707B02)

Plant Regeneration of Leymus chinensis Via in Vitro Culture

LIU Gong-she, WANG En-hua, LIU Jie, QI Dong-mei, LI Fang-fang

Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, China

Received:2002-01-31 Revised:2002-03-28 Online:2002-08-15 Published:2002-08-15

摘要/Abstract

摘要： 采用离体培养方法首次获得羊草体细胞再生植株。其方法要点是:取羊草幼穗为外植体,经0.1%HgCl₂溶液表面消毒后,接种到含2mg/L2,4-D的MS培养基上,置于恒温25℃条件下诱导愈伤组织。在加有1mg/L2,4-DMS培养基上继代2次后,转移到含1.0mg/LKT和0.5mg/LNAA的MS培养基上分化培养得到再生芽。在无激素的基本培养基上获得了生根的试管苗,试管苗移栽到温室后生长正常。研究结果还表明,尽管从羊草叶片、幼穗和成熟胚在同样培养条件下均能诱导出愈伤组织,但只有幼穗愈伤组织能够继续分化出再生植株。羊草试管苗的分化因基因型和外源激素的不同而异。

关键词: 羊草(Leymus chinensis), 幼穗, 外殖体, 再生植株

Abstract: The regenerated plants were obtained from Leymus chinensis for the first time through tissue culture.Immature-base explants were cultured on Murashige and Skoog’s basal medium(MS) supplemented with 2 mg/L 2,4-D after surface sterilized with 0.1% HgCl₂ to induce callus at 25℃ constant temperature.After moved to MS medium containing 1mg/L 2,4-D for two times,the compact calluses were selected and transferred to MS basal medium containing 1.0mg/L Kt and 0.5mg/L NAA,which differentiated into shoot-buds. The shoot-buds appeared and regenerated new roots,finally developed plantlets on 2,4-D-free basal medium. Regenerated plants growth well when transferred into greenhouse.A comparative study of different explants,in this study,revealed that only could the callus from immature stachys differentiate into regenerated plants. The differentiate potential of regenerated plants has close related to the genotype and external hormone.

Key words: Leymus chinensis, Immature stachys, Explant, Plant regeneration

中图分类号:

Q813.1

刘公社, 汪恩华, 刘杰, 齐冬梅, 李芳芳. 羊草幼穗离体培养诱导植株再生的研究[J]. , 2002, 10(3): 198-202.

LIU Gong-she, WANG En-hua, LIU Jie, QI Dong-mei, LI Fang-fang. Plant Regeneration of Leymus chinensis Via in Vitro Culture[J]. , 2002, 10(3): 198-202.

0
/ / 推荐

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://manu40.magtech.com.cn/Jweb_cdxb/CN/10.11733/j.issn.1007-0435.2002.03.009

https://manu40.magtech.com.cn/Jweb_cdxb/CN/Y2002/V10/I3/198

参考文献

[1] Jia S-X(贾慎修).Forage flora in people republic of sinica[M].Beijing:China Agricultural Press,1987,19～35(in Chinese)
[2] Liu G-S(刘公社),Wang Z-Y(王志远).Symposia of continual development research in north agriculture and animal husbandry district[M].Beijing:China Science and Technology Press,1999,136～137(in Chinese)
[3] Ren W-W(任文伟), Qian J(钱吉), Wu T(吴霆), Zheng S-Z(郑师章).Comparative Study on Morphological Anatomic Structure of Leymus Chinensis in Different Geographic Populations[J].Journal of Fudan Uinv.(Natural Science)(复旦学报)(自然科学版).1999,38(5):561～564
[4] Zhao Q-L(赵巧丽), Guan L-X(关力学), Zhu Y(朱宇).Observation on microsporogenesis of ultramicroscopic structure for Leymus chinensis Trin.Tzvel.[J].J Shanxi Med Univ.(山西医科大学学报) 1997,28(Suppl.):87～89(in Chinese)
[5] Li C-H(李春红).Studies on the chromosome karyotype and G-banded chromosomes of Leymus chinensis and Roegneria pendulina[J].Acta Prataculturae Sinica(草业学报),1990,1(1):55～62(in Chinese with English abstrract)
[6] Wang M-L(王梦龙),A Study on Seed Production of Aneurolepidium Chinensis[J].Grassland of China(中国草地),1998,(1):18～20(in Chinese)
[7] Lu P(卢萍),Yu D-N(余诞年),Yang X-L(杨锡麟),Yang S-C(杨锁成).The analysis of peroxidase isozyme of leymus chinensis[J].J Inner Mongolia Normal Univ (Natural Science Edition) (内蒙古师大学报)(自然科学汉文版),1997,2:55～58(in Chinese with English abstract)
[8] LIU J(刘杰),Liu G-S(刘公社),Qi D-M(齐冬梅),Li F-F(李芳芳).Construction of Genetic Fingerprints of Aneurolepidium Chinensis Using Microsatellite Sequences[J].Acta Bot Sin(植物学报),2000,42(9):985～987(in Chinese with English abstract)
[9] Chen H(陈晖),Kuang B-J(匡柏健),Wang J-J(王敬驹).Quality improvement of Aneurolepidium Chinense via cell engineering[J].Acta prataclturae Sinica(草业学报),1995,4(1):1～5(in Chinese with English abstract)
[10] Chen H(陈晖),Kuang B-J(匡柏健),Wang J-J(王敬驹).Studies on amino acid pool levels and changes of proling anabolism in variant line HR20-8 of Chinese aneurolepidium[J].Acta Bot.Boreal.-Occident.Sin(西北植物学报),1996,16(3):208～213
[11] Murashige T and Skoog F.A revised medium for rapid growth and bioassays with tobacco tissue cultures[J].Physiol.Plant.1962,15:473～497
[12] Chen W-P(陈文品), Du C-X(杜丑新), Liu T-Y(刘天育), Wu Q-S(吴琴生), Liu D-J(刘大钧).Direct regeneration of plantlets and micropropagation by immature inflorescence culture of Ryegrass[J].Grassland of China(中国草地),1994,3:65～67(in Chinese with English abstract)
[13] Wang L(王丽), Zou M-Q(邹明谦), Li Y-N(李沿宁), Zhang G-F(张根发).Tissue culture and plant regeneration of Elymus giganteus[J].Pratacultural Science(草业科学),1995,12(6):42～44(in Chinese with English abstract)
[14] Shi Y-J(时永杰), Sun X-P(孙晓萍), Liu X-Q(刘晓强).Tissue culture of young spikelets of Bothriochloa ischaemum[J].Pratacultural Science(草业科学),1998,15(6):19～20(in Chinese with English abstract)
[15] Luo X(罗璇), Wang K-P(王克平), Liu S-J(刘四军).Rapid propagation of Herbages-Tissue culture of Puccinellia chinampoensis[J].Grassland of China(中国草地),1994,4:46～48(in Chinese with English abstract)

羊草幼穗离体培养诱导植株再生的研究

Plant Regeneration of Leymus chinensis Via in Vitro Culture

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 11

编辑推荐

Metrics

本文评价

[1]	赵晓晓, 谢坤良, 贾冬冬, 张舒梦, 孟敏, 孙风丽, 张超, 奚亚军. 柳枝稷幼穗分化过程及其分期[J]. 草地学报, 2019, 27(1): 170-177.
[2]	徐开杰, 史丽丽, 奚亚军, 朱建楚. 柳枝稷基因枪转化法中国产裂解膜代替进口膜的可行性研究[J]. 草地学报, 2014, 22(3): 673-676.
[3]	耿小丽, 魏臻武, 姚喜红, 赵艳. 苜蓿花药培养再生植株染色体倍性检测研究[J]. , 2010, 18(5): 714-718.
[4]	刘露, 胡玉咏, 王兆龙. 高羊茅幼穗分化过程及开花习性的观察[J]. , 2009, 17(2): 197-201.
[5]	梁流芳, 佘建明, 吴瑛瑛, 张保龙, 董民强, 倪万潮. 海雀稗幼穗离体培养植株再生[J]. , 2008, 16(6): 590-593.
[6]	鲍芫, 米福贵. 长穗偃麦草与中间偃麦草杂种成熟胚离体培养与再生体系的建立[J]. , 2007, 15(4): 348-351.
[7]	宋俊双, 王赞, 孙桂芝, 高洪文. 柠条锦鸡儿的组织培养[J]. , 2007, 15(1): 66-69.
[8]	王玉民, 刘艳芝, 夏彤, 李望丰, 李俊波, 董英山. 苜蓿子叶体细胞胚的诱导和植株再生(简报)[J]. , 2005, 13(1): 79-81.
[9]	陈燕, 李聪, 苏加楷, 黄文惠, 吕德杨, 赵广宇. 发根农杆菌介导的百脉根的基因转化[J]. , 1996, 4(2): 116-120.
[10]	陈燕, 李聪, 苏加楷, 黄文惠, 陈受宜, 刘风华. 根癌农杆菌介导的1─磷酸甘露醇脱氢酶基因转化百脉根的研究[J]. , 1996, 4(1): 7-11.
[11]	白静仁, 何茂泰, 袁清, 萨仁, 李永干. 野生黄花苜蓿叶肉原生质体培养和植株再生[J]. , 1994, 2(1): 59-63.