草地学报

›› 1996, Vol. 4 ›› Issue (1): 7-11.DOI: 10.11733/j.issn.1007-0435.1996.01.002

• 研究论文 • 上一篇    下一篇

根癌农杆菌介导的1─磷酸甘露醇脱氢酶基因转化百脉根的研究

陈燕1, 李聪1, 苏加楷1, 黄文惠1, 陈受宜2, 刘风华2   

  1. 中国农业科学院畜牧研究所, 北京100094;
    2. 中国科学院遗传研究所, 北京100101
  • 出版日期:1996-02-15 发布日期:2012-07-11
  • 基金资助:
    国家科委863计划

Transformation of the Lotus corniculatus with Agrobacterium tumefaciencs Carrying the mtlD gene

Chen Yan1, Li Cong1, Su Jiakai1, Huang Wenhui1, Chen Shouyi2, LiU Fenghua2   

  1. 1. Animal Science Institute of CAAS, Beijing 100094;
    2. Genetic Institute of Academia Sinice, Beijing 100012
  • Online:1996-02-15 Published:2012-07-11

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摘要： 本文以百脉根子叶外植体为受体材料,通过根癌农杆菌介导的方法,导入外源目的基因1－磷酸甘露醇脱氨酶(mtlD)基因和npt－Ⅱ基因,建立了转化系统,获得转基因植株,转化频率为9.7%.转化处理的外植体再生植株的茎段在含卡那霉素的分化培养基上膨大,再生成苗和植株。抗性植株的形态正常,移入盆栽后生长良好。PCR扩增与扩增产物的Southern杂交证明外源基因已整合到百脉根基因组上。

关键词: 百脉根, 根癌农杆菌, 基因转化, 再生植株

Abstract: Transgenic plants of Lotus corniculatus L.were obtained by Agrobacterium-mediated transformation.utilizing a disarmed Ti plasmid pBin438 which containing mannitol-1-phosphate dehydrogenase gene mtlD and kanamycin resistance gene npt-II.The transformation frequency was 9.7%.Plants were regenerated from segments of kanamycin-resistant plants on kanamycin containing medium.Plants were morphologically normal and grew well after being transfered to pot.The results of PCR amplification and Southern blot hybridization confirmed that mtlD and npt-II gene were integrated into the Lotus corniculatus L.genome.

Key words: Lotus corniculatus L., Gene transformation, Plant regeneration, Agrobacterium tumefaciens