盐胁迫对芨芨草种子萌发苗Na+,K+含量与质膜H+-ATPase活性的影响

doi:10.11733/j.issn.1007-0435.2010.06.013

›› 2010, Vol. 18 ›› Issue (6): 823-828.DOI: 10.11733/j.issn.1007-0435.2010.06.013

盐胁迫对芨芨草种子萌发苗Na⁺,K⁺含量与质膜H⁺-ATPase活性的影响

董秋丽, 夏方山, 董宽虎

山西农业大学动物科技学院, 山西, 太谷, 030801

收稿日期:2010-04-15 修回日期:2010-10-18 出版日期:2010-12-15 发布日期:2010-12-15
通讯作者: 董宽虎,E-mail:dongkuanhu@126.com
作者简介:董秋丽(1985- ),女,山西阳泉人,硕士研究生,研究方向为牧草抗逆性研究,E-mail:d394875786@126.com
基金资助:
“十一五”国家科技支撑计划课题(2007BAD56B01);农业部公益性行业(农业)科研专项(nyhyzx07-022);山西省科技攻关项目(20080312002-1)资助

Effects of NaCl and Na₂SO₄ Stress on the Content of Na⁺,K⁺ and Plasma Membrane H⁺-ATPase Activity of Achnatherumsplendens Seedling

DONG Qiu-li, XIA Fang-shan, DONG Kuan-hu

College of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China

Received:2010-04-15 Revised:2010-10-18 Online:2010-12-15 Published:2010-12-15

摘要/Abstract

摘要： 以芨芨草(Achnatherumsplendens)种子萌发苗为试验材料,分别以不同浓度NaCl和Na₂SO₄进行胁迫,通过对芨芨草叶片和根系中Na⁺,K⁺含量以及质膜H⁺-ATPase活性进行测定,以探讨盐胁迫对芨芨草中Na⁺,K⁺分布以及质膜H⁺-ATPase活性的影响。结果表明:随着NaCl和Na₂SO₄浓度的增加,芨芨草根系和叶片的Na⁺含量增加,K⁺含量下降,K⁺/Na⁺比值下降,根系中质膜H⁺-ATPase活性增加;在NaCl和Na₂SO₄胁迫下,芨芨草叶片中Na⁺含量显著低于根系,K⁺含量显著高于根系,叶片的K⁺/Na⁺比值均大于1并明显高于根系,根系的质膜H⁺-ATPase活性显著高于叶片;与NaCl相比,Na₂SO₄胁迫下,芨芨草根系向叶片的离子选择性运输系数(TS_K,Na)较高,叶片和根系的质膜H⁺-ATPase活性明显高于相同浓度的NaCl胁迫组。与NaCl胁迫相比,芨芨草对Na₂SO₄胁迫的适应性更强。

关键词: 耐盐性, 芨芨草, Na⁺, K⁺, TS_K,Na, 质膜H⁺-ATPase

Abstract: The content of Na⁺,K⁺ and Plasma membrane H⁺-ATPase activity in leaves and roots of Achnatherumsplendens were determined in order to investigate the distribution of Na⁺,K⁺ and the plasma membrane H⁺-ATPase activity on Achnatherumsplendens under varying levels of NaCl and Na₂SO₄.Results show that with the concentration of NaCl and Na₂SO₄ increasing,the content of Na⁺ increased and K⁺ decreased,then K⁺/Na⁺ ratio decreased in roots and leaves of seedlings in Achnatherumsplendens.The PM H⁺-ATPase activity increased only in roots of Achnatherumsplendens.The content of Na⁺ in leaves was lower than that in roots,and the content of K⁺ in leaves was higher than that in roots under NaCl and Na₂SO₄ stress.The K⁺/Na⁺ ratio in leaves was more than 1 and higher than that in roots.The PM H⁺-ATPase activity in roots was higher than that in leaves.The TS_K,Na was higher under Na₂SO₄ stress than NaCl stress.The PM H⁺-ATPase activity in leaves and roots was higher under Na₂SO₄ stress than the same concentration of NaCl stress.Achnatherumsplendens was more tolerant to Na₂SO₄ stress than NaCl stress.

Key words: Salt tolerance, Achnatherumsplendens, Na⁺, K⁺, TS_K,Na, PM H⁺-ATPase

中图分类号:

S330.29

董秋丽, 夏方山, 董宽虎. 盐胁迫对芨芨草种子萌发苗Na⁺,K⁺含量与质膜H⁺-ATPase活性的影响[J]. , 2010, 18(6): 823-828.

DONG Qiu-li, XIA Fang-shan, DONG Kuan-hu. Effects of NaCl and Na₂SO₄ Stress on the Content of Na⁺,K⁺ and Plasma Membrane H⁺-ATPase Activity of Achnatherumsplendens Seedling[J]. , 2010, 18(6): 823-828.

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盐胁迫对芨芨草种子萌发苗Na⁺,K⁺含量与质膜H⁺-ATPase活性的影响

Effects of NaCl and Na₂SO₄ Stress on the Content of Na⁺,K⁺ and Plasma Membrane H⁺-ATPase Activity of Achnatherumsplendens Seedling

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盐胁迫对芨芨草种子萌发苗Na+,K+含量与质膜H+-ATPase活性的影响

Effects of NaCl and Na2SO4 Stress on the Content of Na+,K+ and Plasma Membrane H+-ATPase Activity of Achnatherumsplendens Seedling

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盐胁迫对芨芨草种子萌发苗Na⁺,K⁺含量与质膜H⁺-ATPase活性的影响

Effects of NaCl and Na₂SO₄ Stress on the Content of Na⁺,K⁺ and Plasma Membrane H⁺-ATPase Activity of Achnatherumsplendens Seedling