PEG胁迫对偏关苜蓿脯氨酸代谢途径关键酶的影响

doi:10.11733/j.issn.1007-0435.2011.06.019

›› 2011, Vol. 19 ›› Issue (6): 1000-1004,1024.DOI: 10.11733/j.issn.1007-0435.2011.06.019

PEG胁迫对偏关苜蓿脯氨酸代谢途径关键酶的影响

朱慧森¹, 刘艳香¹, 董宽虎¹, 杨武德²

1. 山西农业大学动物科技学院, 山西太谷030801;
2. 山西农业大学农学院, 山西太谷030801

收稿日期:2011-07-26 修回日期:2011-10-15 出版日期:2011-12-15 发布日期:2012-07-09
通讯作者: 杨武德,E-mail:sxauywd@126.com
作者简介:朱慧森(1977- ),女,山西晋中人,副教授,博士研究生,研究方向为牧草抗逆生理生态,E-mail:zhuhuisen@126.com
基金资助:
国家“十一五”科技支撑计划课题(2007BAD56B01);国家农业科技成果转化资金项目(2009GB2A300043);山西省青年基金项目(2006021036);农业部草地农业生态系统学重点开放实验室开放课题基金资助

Effect of PEG Stress on Key Enzymes Involved in Proline Metabolism of Medicago sativa cv.Pianguan

ZHU Hui-sen¹, LIU Yan-xiang¹, DONG Kuan-hu¹, YANG Wu-de²

1. College of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China;
2. College of Agronomy, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China

Received:2011-07-26 Revised:2011-10-15 Online:2011-12-15 Published:2012-07-09

摘要/Abstract

摘要： 以山西地方品种偏关苜蓿(Medicago sativa cv.Pianguan)为试验材料,采用20%聚乙二醇(PEG-6000)分别处理不同时间(0,8,16,24,32,48和72h),研究偏关苜蓿地上部及地下部的脯氨酸(proline,Pro)含量、鸟氨酸δ-氨基转移酶(ornithine-oxo-acidtransaminase,δ-OAT)、吡咯啉-5-羧酸合成酶(pyrroline-5-carboxylate synthetase,P5CS)、脯氨酸脱氢酶(proline dehydragenase,ProDH)活性的动态变化。结果表明:PEG胁迫下,地上部Pro含量显著高于地下部,PEG处理40h时,地上部Pro含量最高(3230.4μg·g^-1 FW)。地上部和地下部P5CS活性呈"升-降-升-降"的变化趋势。δ-OAT活性则在胁迫8h时升高,之后下降。ProDH活性受PEG胁迫显著降低,呈时间依赖性。PEG胁迫前期,偏关苜蓿的Pro积累以鸟氨酸-脯氨酸途径为主,随胁迫时间的延长,逐渐转为以谷氨酸-脯氨酸途径为主。

关键词: 偏关苜蓿, 脯氨酸, 鸟氨酸δ-氨基转移酶, 吡咯啉-5-羧酸合成酶, 脯氨酸脱氢酶

Abstract: Medicago sativa cv.Pianguan is an important local variety in Pianguan of Shanxi province.This study focuses on the dynamic changes og proline(Pro) content and the activities of pyrroline-5-carboxylate synthetase(P5CS),ornithine-oxo-acid transaminase(δ-OAT) and proline dehydragenase(ProDH) in both aboveground and underground parts of Medicago sativa cv.Pianguan under PEG-6000 stress for 0,8,16,24,32,48 and 72 h,respectively.The proline content of aboveground parts was the highest(3230.4 μg·g^-1 FW) when stressed for 40 h,which is significantly greater than that of underground parts.P5CS activity showed a tendency of rising-declining-rising-declining,while the δ-OAT activity was rising initially when stressed for 8 h,then declining.ProDH activity significantly declined for both aboveground and underground parts with increasing PEG-6000 stress time.In the earlier stage of PEG stress,the ornithine-proline pathway is the main style for Medicago sativa cv.Pianguan responding to PEG stress,but the glutamate-proline pathway would gradually take over the predominant role with the time extending of PEG stress.

Key words: Medicago sativa cv.Pianguan, Proline, Ornithine-oxo-acidtransaminase, Pyrroline-5-carboxylate synthetase, Proline dehydragenase

中图分类号:

Q945.78

朱慧森, 刘艳香, 董宽虎, 杨武德. PEG胁迫对偏关苜蓿脯氨酸代谢途径关键酶的影响[J]. , 2011, 19(6): 1000-1004,1024.

ZHU Hui-sen, LIU Yan-xiang, DONG Kuan-hu, YANG Wu-de. Effect of PEG Stress on Key Enzymes Involved in Proline Metabolism of Medicago sativa cv.Pianguan[J]. , 2011, 19(6): 1000-1004,1024.

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PEG胁迫对偏关苜蓿脯氨酸代谢途径关键酶的影响

Effect of PEG Stress on Key Enzymes Involved in Proline Metabolism of Medicago sativa cv.Pianguan

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